Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,887	G→A	A293A (GCC→GCT)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,887	0	G	A	100.0%	73.1 / NA	25	A293A (GCC→GCT)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/18);  total (7/18)

CCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATC  >  NC_000913/1829643‑1830133                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ccttgccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGGTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGAcc                                                                                                                                                                                                                                                  <  2:143806/251‑1 (MQ=255)                                                 gcgcCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCTCCCTTCGCAGGCTGCCCTTCTGCCACTTcc                                                                                                                                                                                                  <  2:312671/251‑1 (MQ=255)                                                                aTCCAGTCACCGTTAATCCATAAAGTCATGCTTTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTa                                                                                                                                                                                   <  1:72230/251‑1 (MQ=255)                                                                   cAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCCCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGGCTTTTACgg                                                                                                                                                                                >  1:248676/1‑251 (MQ=255)                                                                             tAATCCATAAGGTCGTGCTGTTTTCTACTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGATAACATCAAACGCCCGGTTGCCGGATCGGTTAGCAACCGCACCCCGCGGGAATGGTGGTAATTTTTCTTAGCGAACAGGCAGGCAGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCCCCAGCCGCCTTTTACGGAGGgcgcgc                                                                                                                                                                       >  2:314490/1‑250 (MQ=255)                                                                                          cattcTGTTTTCTCCTCTGCGCCCAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACTCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCTCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGt                                                                                                                                                          <  2:192710/246‑1 (MQ=255)                                                                                                   ttCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCaa                                                                                                                                                 <  1:310650/250‑1 (MQ=255)                                                                                                          ctGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGGACGCACCAGCACCACGCGGAAATGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGAGGGCGCGCACGCGGGCGATGGCACCCTCAAGCggcggc                                                                                                                                         >  1:111253/1‑251 (MQ=255)                                                                                                                acaGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGGCGGAGCGGTACGAACCAGCACCACGCGGGAATGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAAGCTGCCCTTCTTCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCCCGCGGGAGATGGCAACCTCAAGCGGCGGccccccc                                                                                                                                   >  1:118939/1‑247 (MQ=255)                                                                                                                                    cgATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAAGCTGCCCTTCTGCCCCTTCCACCAGCCGACTTTTACGGATGGCGCGCCCGCGGTCGATGTAACACTCAAGCGTCGGCCCACCGTCAAAAGTGTCGATATTGtt                                                                                                               >  1:108652/1‑251 (MQ=255)                                                                                                                                      aTCCCCGGCGTGTCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTAc                                                                                                             <  2:111253/251‑1 (MQ=255)                                                                                                                                         cccGGTGTGGCATTTGTGGGCTTCTAGTTGTGAGTCGGTTAAAATCAAACGCTCGGTTGCAGGATCGGTACGCACCATCACCACGCGGAAATGGTGATAATGTTCATTAGCGACCAGGCAGGCTGGGAAATCTCCCTGCTCTGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTAAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGt                                                                                                          <  2:177114/251‑1 (MQ=255)                                                                                                                                          ccGGCGTGGCAGTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTa                                                                                                         <  2:254739/251‑1 (MQ=255)                                                                                                                                                           ggCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGTCCCCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAATCCttctttct                                                                                        <  1:221650/251‑1 (MQ=255)                                                                                                                                                              aTCCAGTTGTGCGGCGGTTGAAATCAAACGCTCGGGTGCCGGGTCGGTACGCCCCAGCACCACGCGGAAATGGTGATAATATTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGGTGGCGCGCACGCGGTCGATGTCACCATCCAGCGTCCGCCCCCCCTCAAAGATGTCGGTACAGTTTCGGTAGCGAAAACCttctttccccc                                                                                     >  2:272009/1‑247 (MQ=255)                                                                                                                                                              aTCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTGGCCGGATCGGTACGCACCAGCTCCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTGTCTCCa                                                                                     <  1:225471/251‑1 (MQ=255)                                                                                                                                                                             gtttAAAATCAAACGCTCGGGTGCCGGATCGGTACGCCCCAGCCCCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAg                                                                      <  1:8178/249‑1 (MQ=255)                                                                                                                                                                                         ccGCTCGGTTGCCGGATCGGTACGCCCCATCACCACGCGGAAAGGGTGATAATGTTCATGAGCGACCAGGCAGTCTGGGAAATCGCCCTTCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGTATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATAGAGGTACGGTAGCGAAAACCTTCGTTCTCCAGCATCGCGCGGGCAGGGGCGGTTTGCg                                                          <  2:143685/250‑1 (MQ=255)                                                                                                                                                                                                               cGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTc                                    <  1:192407/251‑1 (MQ=255)                                                                                                                                                                                                                             ccggcaagtggtaTAATTTTCATTAGCGACCTGGCAAGCTGGGAACTCGCCCGGCGCAGTCTGCCCGTCTGCCATGTCCACCATCCGACTTTTACGTATGTCGCTCACGCGGCCGTTGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGAGATATTTACTGTAGCGATAGCCTTCTTTCTCCGGCACCTCCCGGGCAGGCGCGGTTTGCGTATGTACCTGACCCATGTCTTCCTgttcttgctggg                      <  2:138202/239‑12 (MQ=255)                                                                                                                                                                                                                                gAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTggg                      >  1:167685/1‑248 (MQ=255)                                                                                                                                                                                                                                gAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTggg                      <  2:167685/248‑1 (MQ=255)                                                                                                                                                                                                                                        gatcaTTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTCCGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTg            <  2:91349/246‑1 (MQ=255)                                                                                                                                                                                                                                        gATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTg            <  1:86476/250‑1 (MQ=255)                                                                                                                                                                                                                                                 cATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATc  <  1:196945/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATC  >  NC_000913/1829643‑1830133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑