Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,728,514	C→A	T56T (ACC→ACA)	rnt →	ribonuclease T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,728,514	0	C	A	95.2%	58.5 / ‑2.0	21	T56T (ACC→ACA)	rnt	ribonuclease T
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (15/5);  total (15/6)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-01

GCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGCCGCCGCAGA  >  NC_000913/1728275‑1728747                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          gCATCTTTACTGCATCGACAAGTAATATTGGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAAACAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTGTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGTTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCTGGCACAACAGTACAttt                                                                                                                                                                                                                                <  2:216254/251‑1 (MQ=255)                      gTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCgg                                                                                                                                                                                                           >  1:166090/1‑251 (MQ=255)                                tCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTg                                                                                                                                                                                                 >  1:168390/1‑251 (MQ=255)                                          cgcTGCAATTTAGCCGTATTAAGAGAATCAGATGTCCGATAACGCTTAACGTTCCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAg                                                                                                                                                                                       <  2:228789/251‑1 (MQ=255)                                                                   aaTCAGATGTACGATAACGCACAACTTACCGGTCTGTGCGACCGTTTTAGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGATGATGCAGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCATCGCCTTCAACGGCATTGActc                                                                                                                                                              >  1:282440/1‑249 (MQ=255)                                                                      cAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGa                                                                                                                                                            <  1:9965/250‑1 (MQ=255)                                                                             ccgttaacgataaaattACAGGTCTGTGCGACCGTTTTCGTGGTTTTTATCATGTTGCGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCCACACCCTGACAATGGCTGAACAAGGCTGGCTGATCCCGGACCAAACATTACATTTCCACGTCGAACCCTTTGTCGGCGCAAATTCGCAACCAGAAGCCACCCCATTCAACGCCCTTGACCCGACCGCTccc                                                                                                                                                    >  2:54429/16‑251 (MQ=255)                                                                                                cGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCg                                                                                                                                 >  2:71720/1‑251 (MQ=255)                                                                                                               ttttCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGc                                                                                                                  >  2:325676/1‑251 (MQ=255)                                                                                                                            tATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTa                                                                                                      >  1:169681/1‑250 (MQ=255)                                                                                                                             aTCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTaa                                                                                                     <  2:169681/250‑1 (MQ=255)                                                                                                                                         tCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCTGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTaa                                                                                         >  1:3957/1‑250 (MQ=255)                                                                                                                                            atgttgccaCAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGCTTGCCGCCATCACCATGAAAATGGCTGAAAAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTAGGCGCAAATTTGCAACCAGAAACCCTCGCCTTCAACGGCATTGACCCGCACGATCCCTCACGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGACATTTTTAAAGTTGAACGTAAAGGt                                                                                     >  2:130961/9‑251 (MQ=255)                                                                                                                                                  aaaCAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTaa                                                                                >  1:262596/1‑250 (MQ=255)                                                                                                                                                         cGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAagcgagcg                                                                        <  1:325676/251‑1 (MQ=255)                                                                                                                                                                                 gctgctTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACCTTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCCTTAtggtgg                                                >  1:333764/1‑251 (MQ=255)                                                                                                                                                                                      ttGAGATTGCCGCCATCACCCTAAAAATGGATGAACAAGGCTGGCGAATGCCGGACACAACATTACATTTCCACGTCGCACCATTAGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCATTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGCCAGCGAATACGAGGCGCTGCACGACATTTTTAAAGGTGTGCGTCAAGGAATTAAAGCGACCGACTATAACCGCACCCTTATTGTGGCGcac                                           >  1:191412/1‑251 (MQ=255)                                                                                                                                                                                                  ccATCACCCTGAAAATGGATGAACAAGGCGGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCCCAATGCCAAtttt                               >  2:263932/1‑251 (MQ=255)                                                                                                                                                                                                   cATCACACTGCAAATGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACCATCCCGCTCGCGGCGCGGTCAGCAAATACGAGGCGCTGCACGCAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGAAACCGCGCCATTATGGTGGCGCTGAATGCCAATTTTg                              >  1:290639/1‑251 (MQ=255)                                                                                                                                                                                                                aTGGATGAACAAGGCTGGCTGATGCCGGACACAACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTat                 >  1:314852/1‑251 (MQ=255)                                                                                                                                                                                                                               gggctgATGCCGGACACAACATTACATTTCCACGGCGAACCATGTGGCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGATTTATGATGGCCGCCGCAGa  <  1:29476/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          GCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATTTGTCGGCGCAAATTTGCAACCAGAAGCCCTCGCCTTCAACGGCATTGACCCGAACGATCCCGATCGCGGCGCGGTCAGCGAATACGAGGCGCTGCACGAAATTTTTAAAGTTGTACGTAAAGGTATTAAAGCGAGCGGCTGTAACCGCGCCATTATGGTGGCGCACAATGCCAATTTTGATCACAGCTTTATGATGGCCGCCGCAGA  >  NC_000913/1728275‑1728747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑