Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,358,8590TC89.3% 67.1 / ‑5.4 28Y110C (TAT→TGT) puuPputrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (9/16);  minor base A (2/1);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

ACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAG  >  NC_000913/1358616‑1359094
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
acggagcaaaCGCCTTCTCCTTTATGCAGTCCCCGAACTACCAGGAAGATAAACACCACCGTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCCCTTCAGATTCGCGGCGGTTAAAAGGGCGACGAAGGTTACCACCCACACCCACGGCGGCACGTCCGGGAAGAGGACAGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGAAAATCcag                                                                                                                                                                                                                                       <  2:228627/243‑1 (MQ=255)
   gTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACTCCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCcagcag                                                                                                                                                                                                                                    <  2:100624/251‑1 (MQ=255)
           gcattCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGATACAATCCAGCAGCGATGAcc                                                                                                                                                                                                                            <  1:269311/248‑1 (MQ=255)
                  ccTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGcc                                                                                                                                                                                                                        <  1:200887/248‑1 (MQ=255)
                     ttATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATGTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAGGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCGAGCAGCGATGACCAGCCGAcc                                                                                                                                                                                                                    <  2:76723/249‑1 (MQ=255)
                           aGTCCCTGAACTACCAGGAAGATAAACAACACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATcc                                                                                                                                                                                                            <  2:155884/251‑1 (MQ=255)
                                   aaCTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACAAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAAGTTACCACCCCCACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGAATTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCCGCCGACCATAAATCCGACGTGCg                                                                                                                                                                                                    >  1:200211/1‑251 (MQ=255)
                                      tACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACCGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGACGAAGGTTAACACCCACACCCCAGGCGGCACTTCCGGGAAGAGGGGGGAGAGACAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAAGAGACAAACCAACAGCGATGACCAGCCGACCAAAAATCCGACGTGCgggg                                                                                                                                                                                                 >  1:325666/1‑250 (MQ=255)
                                       aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACCACCACGGCGGGCCTTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGCTAATCATCCGCAAAAAGAGACAATCCAGCAGCGCTGACCCGCCGACCCTAAATCCGACGTGCGGGtt                                                                                                                                                                                                >  1:277634/1‑251 (MQ=255)
                                          aGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGGCGAAGGTTACCACCCACACCCCCCGCGGCACTTCCGGGAAGAAGGCGGAGAGATAGATTTTCGCCAACACGACGTTAATCATCGGCAAAAAGAGACAATCCAGCCGAGATGAACAACCGACCATAAATCCGCAGTGCGGGGTAAt                                                                                                                                                                                             >  1:233254/1‑251 (MQ=255)
                                           ggAAGATAAACGCCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCGTCAGATTCGGGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGGACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCACCAACAAGACGTTATTCAGCGGCAAAAAGATACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGTGGTAATc                                                                                                                                                                                            <  2:194374/251‑1 (MQ=255)
                                                                                  cAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAATGTTACCACCCACCCCCACGGCGGCACTTTCGGGCAAAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAAAAACCATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTT‑CGCGTAGGTATAGGCCGAACCGGCCTCCggc                                                                                                                                                    >  1:256349/1‑250 (MQ=255)
                                                                                            aTTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTGTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGaa                                                                                                                                          <  2:59568/251‑1 (MQ=255)
                                                                                                        gACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGGGGCACTTCAGGGAAGAGGGGGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATAGGCAAAAAAAGAAAATCCAGCAGCGGTGACCAGCCGACCATAAATCCGACGTGCGGGGTAATTGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGGACCAGTTTGCCGt                                                                                                                              >  1:137636/1‑251 (MQ=255)
                                                                                                          gcAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACAGCTACGGCGGCACGGCCGGGAAGAGGGCGGAGAGATAGATTTGCGCCAAAAAGACGGTAATCATCGGAAAAAAGAGACAATCCAGCAGCGATGACCAGCCGAGCATAAATCCGACGTGCGGGTTAATCGACTGTTG‑CGCTTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAg                                                                                                                            <  1:29393/250‑1 (MQ=255)
                                                                                                                  aCGCTCTTCAGATTCGCGGCGGTTAAAATGGGGGCGAAGGTTACCACCCCCACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCCGCAGCGATGACCAGCCGACCATAAATACGACGTGCGGGGTAATCGGCTTTTG‑CGCGTAGGGATAAGCCGAACCCGCCCCCGGAAACTGGCGAACCAGTTTTGCGCAGCTGATaac                                                                                                                    >  2:261677/1‑249 (MQ=255)
                                                                                                                     ctctTCAGATTCGCGGCGGTTTAAATGGCGACGAAGGTTACCACCCACACCAACGGCGGCACTTCCGGGAAGAAGGCGGGGAGATAAATTTTCGCCAAAAAGAAGTTAAACATCGGCAAAAAGAGAAAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTGGGTATAGGCCGAACCGGCCTCCGGGAACTGGCGAACCAGTTTGCCGTAACTGATAGCGGt                                                                                                                 >  1:10299/1‑251 (MQ=255)
                                                                                                                       ctTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGt                                                                                                                 <  1:37351/249‑1 (MQ=255)
                                                                                                                            gATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCCCGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATATCGGTAAACAGc                                                                                                          <  2:277634/251‑1 (MQ=255)
                                                                                                                                     gggTTAAAATGGCGACGAAGGTTACCACCCCCCCCCTCGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCACCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCGGAACCGGCCTCCGGAAACTGGCGACCCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgcc                                                                                                 <  1:216653/250‑1 (MQ=255)
                                                                                                                                                                   acacccacGGCGGCACTCCGGGGAAGGGGGGGGAGAGATAGATTTGCGCCAACAAGACGTAAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCAAAAATCCGACGTGCGGGTTAAGCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGa                                                                   <  1:261677/251‑1 (MQ=255)
                                                                                                                                                                        cacGGCGGCAATTCCGGGAAGAGGGCGGAGAGATAGATTTCCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAAGCCGACGTGCGGTTTAATCGACTTTGG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAACCTGGCGACCCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTc                                                              <  1:87082/251‑1 (MQ=255)
                                                                                                                                                                                          aaGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTACTCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAAt                                            >  2:115429/1‑251 (MQ=255)
                                                                                                                                                                                                     gagaAAGATTTGCGCCAACAAGCCGTTAATCATCGCCAAAAAGAGACAATCCAGCAGCGAGGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTAt                                 <  1:79498/251‑1 (MQ=255)
                                                                                                                                                                                                                              ttAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGCCCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGAt         >  2:74074/1‑250 (MQ=255)
                                                                                                                                                                                                                                aaTCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAgg      >  1:170103/1‑251 (MQ=255)
                                                                                                                                                                                                                                aaTCATCGGAAAAAAGATACAACCCAGCAGCGATGACCAGCCGCCCATAAATCCGTCGTGCGTGTTAATCGA‑TTTTGTCGCGTATGTAGAGGCTGACCCGGCCCCCGGAACCGGGCAAACCAGTTTGCTGTAGCTGATAGCGGTAAACAGCCCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGTAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAg       <  1:27207/250‑1 (MQ=255)
                                                                                                                                                                                                                                    atcgcaaaaaaGAGACAATCCAGCAGCGATGCCCAGCCGACCAAAAACCCGACGGGCGGGTTAACCGACTTTTG‑CGCTGAGGTATAGGCCGAACCGGCCTCCGGAAACGGGCGACCCGTTTTGCCGTAGCGGATAGCGGTAAACAGCACACCCTCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTAGCAAATACGGTCATCGGCGTTAGAGAGGCCAg  <  2:325666/245‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
ACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTG‑CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAG  >  NC_000913/1358616‑1359094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑