Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,674	G→A	G282R (GGG→AGG)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,674	0	G	A	97.4%	114.6 / NA	38	G282R (GGG→AGG)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/20);  minor base C (1/0);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCAATTTTTACCAGTACG  >  NC_000913/1336434‑1336916                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   ccgtgctgtcgggATACACTCGTGGGTACTGCCGCGCACCCCACCATGAGCAACGGCCTGGGCGGGTGGGGGTGGGGCGTTGGTGGGAGCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGTTTTCCATGCTTATCCGGGATTTAGTGGGCTACAAACTTACCGTAAAATTCCGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATtcgt                                                                                                                                                                                                                                          <  2:5000/240‑1 (MQ=255)      ttatcaggatccattcttttgTACTGACCCGCACACCACCAGGATCAGTGGCCGTGGCGGGCTGGGTGGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGTTTTCCATGCTTACCCCGGATGTAGGGGGCTGCAAACTTACCGGAAAATGACGGGAAGGTTTTACCGCCACAGACCTGGTTCTCACTGTTACCCAAAGGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGaa                                                                                                                                                                                                                                      <  2:143200/230‑1 (MQ=255)        aTCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATtcgt                                                                                                                                                                                                                                          >  1:5000/1‑244 (MQ=255)           cGGATACACTCGTTGGTACTGACTCGCACACCACCATGGTCAACGGCCTTGGCGTGCTGGGGGGGGGCGTTGGGGGGATCGAAGCAGCAGCCGCAATGTTAGCCCCGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGCAAATTACGTGCAGGTATTACAGCCACAGACCTGGTTCTCACTGTTACCCAACTGCTACGCAAACATGGCGTGGTGAGGAAATTCAGCGACTTTTAt                                                                                                                                                                                                                                >  2:132042/1‑251 (MQ=255)            ggATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGAGGGATCGAAGCAGAAGCCGCCATGTTAGGACAGCCGGATTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCGGCGCAAACATGGCGTGGAGCGGAAATTCGTCGAATTTTATg                                                                                                                                                                                                                               >  1:44340/1‑251 (MQ=255)                   cTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGAGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATgg                                                                                                                                                                                                                        >  1:17208/1‑251 (MQ=255)                                 cgctgccaccacattgtcgaagggCCTTGCCGGGTTGGGGCGGGGGGTGGGGGGGATCGAAGCAGAAGCGGCAAGGTTAGGCCAGCCGGTTTCCTTTCTTACCCCGGAGGTAGTGGGCTTCAATCTTCCCGGAAAATTCCGTGAAGGTATGACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGAGTCACTAc                                                                                                                                                                                                          <  2:220559/229‑1 (MQ=255)                                           cccaTGATAAACGGCTGTGGCGTGGTGGGGTGGGGCGTTTGTGGGATCAAAGCAGAAGCCCAAATGTTAGGCACGCCGTTTTCCATGCTTACCCCGGATGTAGGTGGCGTCAAACTTACCGGAAATTTCCGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATTCTGCGCAAACATGGCGTGGTGAGGAAATTAGTCGAATTTTATTGTGATGGGCTGGATTCACTACCGGTGGCGGa                                                                                                                                                                                                <  2:223980/250‑1 (MQ=255)                                           accaTGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCgg                                                                                                                                                                                                 >  1:282860/1‑250 (MQ=255)                                              aTGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGCAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATcg                                                                                                                                                                                             >  2:313762/1‑251 (MQ=255)                                                gATCACCGGCCTTGGGGTGCGGGGGGGTGGCGTTGGTGGGACCGAAGGAGAAGCCGCAATGTGAGGCCAGCCGGTTTCTATGCTTATCCCGTATGTATGGGGCTTCAAACTTCCCGGAAAATTGCTTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGTCGTGGTGAGGAAATTTGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATcgcg                                                                                                                                                                                           <  1:193396/251‑1 (MQ=255)                                                gATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACCGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACCTGGCGTGGTGAGGAAATTAGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATcgcg                                                                                                                                                                                           >  2:234320/1‑251 (MQ=255)                                                    aaCGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGccac                                                                                                                                                                                       >  2:219680/1‑251 (MQ=255)                                                      cGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCACGccacc                                                                                                                                                                                      >  1:226870/1‑250 (MQ=255)                                                                           gggCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAat                                                                                                                                                                <  1:298237/251‑1 (MQ=255)                                                                                          cGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCtgtg                                                                                                                                                 >  1:33961/1‑251 (MQ=255)                                                                                           gAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTgg                                                                                                                                                <  1:313762/251‑1 (MQ=255)                                                                                                           tGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                                                                                                >  1:177544/1‑251 (MQ=255)                                                                                                               gggcaaGCCGGTGTCCATGCTTATCCGGGATGTAGGGGGCTTAAAACTTACCGGAAATTTACGTGAAGTTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCTTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTg                                                                                                                            <  1:35064/246‑1 (MQ=255)                                                                                                                      ccGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCt                                                                                                                     >  2:220592/1‑251 (MQ=255)                                                                                                                                 gCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGAAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGc                                                                                                          <  2:43284/251‑1 (MQ=255)                                                                                                                                                         cAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGAt                                                                                   <  1:33759/250‑1 (MQ=255)                                                                                                                                                          aaaCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATc                                                                                  >  2:59606/1‑250 (MQ=255)                                                                                                                                                               aaCCGAAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCg                                                                            <  1:219680/250‑1 (MQ=255)                                                                                                                                                                 ccGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCACTCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCg                                                                            >  1:109576/1‑249 (MQ=255)                                                                                                                                                                   ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGt                                                                                                        >  1:250007/1‑219 (MQ=255)                                                                                                                                                                   ggAAAATTACGGAAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGt                                                                                                        <  2:250007/219‑1 (MQ=255)                                                                                                                                                                                       tACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAACTTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCaa                                                      >  2:27139/1‑249 (MQ=255)                                                                                                                                                                                              acaGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAggg                                             <  1:129078/251‑1 (MQ=255)                                                                                                                                                                                                                gTTAACCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCg                             <  1:182610/249‑1 (MQ=255)                                                                                                                                                                                                                    cccAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGAt                       <  1:220592/251‑1 (MQ=255)                                                                                                                                                                                                                    cccAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCAGGTGGCGTAACCCGGGCGAt                       <  1:83151/251‑1 (MQ=255)                                                                                                                                                                                                                        aaTGCTGCGCAAACAATGCGTTGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGTCGGATCGCGCCACCATTGCCCATATGACGCCAGAATATTGTGCCACCTGTGGCTTCTTCCCAATCTATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCATGGCATGTGGCGTAACCCGGGCGATGagc                   <  2:182628/251‑3 (MQ=255)                                                                                                                                                                                                                           gctgcGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCaa                <  1:94299/251‑1 (MQ=255)                                                                                                                                                                                                                             tgcGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCAAtt              >  1:70043/1‑251 (MQ=255)                                                                                                                                                                                                                              gcgcAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCAAttt             <  1:234320/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaaCATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCAACATTGCCACTATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAACGCGCAGGGCATGTGGCGTACCCCGGGCGGTGAACCAATTTTTAc         >  2:275083/1‑251 (MQ=255)                                                                                                                                                                                                                                         gCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCAATTTTTACCAGTACg  <  1:94033/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   ATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCAATTTTTACCAGTACG  >  NC_000913/1336434‑1336916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑