| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 53.3% | 3.1 / 11.2 | 16 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (8/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGGGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCG‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAAT‑GCCAGTTCAGG‑ATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACCAGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGT > NC_000913/646499‑646994 | cGGGGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCCATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGACAATTAATATTTCCAAATTTATCACGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGtgc > 2:11643/1‑249 (MQ=255) tCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGAGGATCGCAAGGCCCCCTTTTT‑CAATAATAGTTGGGCACCCCGCTGTAGCCCGCGCCGGCCCCCCTCGccc > 1:334382/1‑250 (MQ=255) aTACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTTG‑GATGCCCCCCTTTTGTACAACTGTTTTCGTCTCGCGCTGTAGCAACCGCAGGgcccccccccccccccgcgcagcaacg > 1:6320/1‑230 (MQ=255) aaTATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCG‑GATGCCCCCCTTTTTCAATAATGTTTTCGCCTCCGGCTGTAGCCACCGCAGGGCCCCCCCGCCACCCCCCGCGGGAA‑CGTTGGTATCGCCGTTGAttgc > 1:262202/1‑248 (MQ=255) aaTATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCG‑AAAGCCCCCCTTTTGCAATAATGTTTTCGCCTCCCGCTGTAGCCACCGCCGGCCCCCCCCGCCCCCCCGCGCTGCCA‑CGTTgggtttgccgctgttccc > 1:218678/1‑235 (MQ=255) aaaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCG‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAg < 1:245280/251‑1 (MQ=255) tATCAAGTTCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCCCTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCG‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGc < 2:175157/250‑1 (MQ=255) aGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGG‑GAAGCCCCCCTTTTTCAATAAAGTTTTCGCCTCCCGCTGTAGGCCGCGCAGGCCCCCCCCGGCACCGCCCGAGGCAAAGGTTGG‑ATAGCCGTTGATCggcctccgatagggccaggg > 1:234595/1‑231 (MQ=255) aataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCG‑AATGCCCCCTTTTTTCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCCCGCCCCCCCGCGATGCCA‑CGGTGGTATCGCCGCTGGTCGCCATCCGTAGGAGGAAGGGATCGAGCa > 1:93377/1‑250 (MQ=255) cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCG‑AATGCCCCCCTTTTTCAATAATGTTTTCGCCTCGCGCTTTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGGTGCAA‑CGTTGGGATCGCCGTTGTTCGCCCTCAGTAGGAGCAAGGGATCGAGCCAT‑GCCCGCTCAGG‑GTCTAAcccccc > 1:69882/1‑249 (MQ=255) gATCAGTAGGTCAGCACTGCCGACTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCG‑AATGCCCCCCTTTTGCAATAATGTTTGCGCCTCGCGCGGTAGCCAGCGCAGGCCCCCCTCCCCACCCCGCGCTGCAA‑CGTTGGGATAGCCGGTGGTTGCCCCCAGTAGGGGGAAGGGATTGAGCAAA‑GCCCGTTAAGG‑GTCTAAcccccgcaccagcc > 1:85743/1‑241 (MQ=255) aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCG‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAAT‑GCCAGTTCAGG‑ATCTAACCCCTGATCCAGCAGAGTGAg < 1:118753/251‑1 (MQ=255) aCTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCG‑GATGCCCCCTTTTTTCAATAATGTTTTCGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCTCCCCACCCCCCCATGCAAACGT‑GGGATCTCCGGTGGTCGCCCTCAGTAGGAGCAAAGTATTGAGGAATTGCC‑GTTCAGG‑ATTTTAACCCTGCTCCCGCAGGGGGGGGTAATGCg > 1:109767/1‑251 (MQ=255) tcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCG‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAAT‑GCCAGTTCAGG‑ATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCAcc < 2:218678/250‑1 (MQ=255) cgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCG‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAAT‑GCCAGTTCAGG‑ATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACCa < 1:180838/250‑1 (MQ=255) gATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCG‑ACTGCCCCCCTTTTTCCATAATGTTTGCGCCCCGCGCTGTAGCCCGCGCAGGGCCCCCTCCCCACCCCGCGATGCAA‑CGTTTGTATCCCCGGTGGTCGGCCTCCGTCAGAGCAAGGGATCGAGCAAA‑GCCAGTGCAGGGACATAACCC‑TGATCCCACAGAGTGAGGTAATGGGGCAAGGCGGGGTTGATCACCatcgta > 2:12833/1‑245 (MQ=255) gggTTCG‑AATGCCCCCTTTTTGCCATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAAT‑GCCAGTTCAGG‑ATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGTTTGATCACCAGTGGATAACCCGCTTCTGCTTCCCCGCGTGCGCCGGTAAGGCCAAGCTGt > 2:298775/1‑251 (MQ=255) | CGGGGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCG‑AATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAA‑CGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAAT‑GCCAGTTCAGG‑ATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACCAGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGT > NC_000913/646499‑646994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |