Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,429,986	IS3 (–) +5 bp :: +T	coding (34‑38/258 nt)	yrdB ←	DUF1488 domain‑containing protein YrdB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	50 (1.600) +T	38/484	0.1	98.0%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	3429986 =	1 (0.030)					coding (38/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB
*	?	NC_000913	1094245 =	NA (NA)	35 (1.120)	33/484	0.1	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	= 3429990	0 (0.000)					coding (34/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB

TAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTT  >  NC_000913/1094008‑1094481                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               tAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCGATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACGTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCAc                                                                                                                                                                                                                                    <  2:28937/250‑1 (MQ=255)                                          gATAATCCTAAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAAt                                                                                                                                                                                        >  1:267680/1‑251 (MQ=255)                                                    cctACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGa                                                                                                                                                                               >  2:224478/3‑250 (MQ=255)                                                     aTACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACt                                                                                                                                                                             >  1:186967/1‑251 (MQ=255)                                                            ataCCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccg                                                                                                                                                                      >  1:276398/1‑251 (MQ=255)                                                                           aCGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGCATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAGGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTgc                                                                                                                                                       >  2:253230/1‑251 (MQ=255)                                                                                                  gacacAGGAAAATGCGGTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTGTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAAGATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAAGTGTTCCGGACTGAGgc                                                                                                                                                                        <  1:115121/207‑1 (MQ=255)                                                                                                  gACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCCTTTTGATCCTACCACCGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGCCTGAGgc                                                                                                                                                                        >  2:115121/1‑211 (MQ=255)                                                                                                     tcaggcacaTGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGCGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGAATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCAGCCACACCAACTGTGCCGCAGCCACCGATTGTAAtaaccttc                                                                                                                             >  2:272712/9‑244 (MQ=37)                                                                                                       aGGAAAATGCGTTAAACCCAAAGGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGGGAAAGAGACCGGGCGTAATAAGGTGTTTGGGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGa                                                                                                                           <  2:186967/251‑1 (MQ=255)                                                                                                        ggAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATAACATTCGat                                                                                                                          >  2:2308/1‑251 (MQ=255)                                                                                                         gAAAATGCGTTAAACCCAATTGAGATCTATCGCCTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAGGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGCGTCCCCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCCCCCCCCCAACTGTGCAGCCGCCACCGTTTGTAATCACATTCGat                                                                                                                          >  2:17254/1‑250 (MQ=25)                                                                                                                        ccAAAGGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt                                                                                                          <  2:140566/251‑1 (MQ=255)                                                                                                                           aaTGAGTTCTTTCGACTGGCTGAATACGCACTTTACGAGGCAAAAAAGACCGGGCGTAATAAGGTGGTTGTGAGGGATTGGGTGAATTTTTGTGAGTCACCAAAAAGCGGCTTTTTGATCCTGCCAGCGTAAGATGGAC‑CCGCCGCTACGGTA‑GGTTCTTTGTTGAAAATGGTTCCGGACGGAGGCCGCTACACCAAGTGGGCCGCCGCCGCCGATTGTAATCCCATTCTAGATAATGAAACACCGTTGcc                                                                                                       <  2:270192/251‑1 (MQ=255)                                                                                                                                     aTCGACTGGCTGAAAACGCACTTTACGAGGCGAAAGAGACCGGGCTTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCCCCATAAAGCGGCATTTTGATCCTACCCATGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCattatt                                                                                              <  2:312504/250‑1 (MQ=255)                                                                                                                                                      gCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAgtgt                                                                            >  1:128048/1‑251 (MQ=255)                                                                                                                                                                                       aagggtgtggtgaggattgtgGTGAATTTTTGTGAGTCACAATAAAGCGGCATTTTGACCCTACTCACTTAATATGGAAACAGGC‑CTAAACGA‑GGTTTTTGTTTCCAAATTGTTCCGGATGGAGTCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACCCCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGTATACATTCCCCTTTCAGCGAATGa                                           <  2:66748/234‑1 (MQ=14)                                                                                                                                                                                        aGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAACGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGa                                           >  1:155547/1‑250 (MQ=255)                                                                                                                                                                                              ttGTGAGGCATGCGGGGAATTTTTGAGCGCCACCATCCAGCCGCATTTTGATCCTCCCCCCGTAATATGCACACACGC‑CTACGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGGCAGAGGCCGCCACACCAACTCTGCCGCCGCCACCGATGGTACTCACGTTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGc                                    >  2:162975/1‑251 (MQ=11)                                                                                                                                                                                                          tggtgAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACAtt                                                            >  1:199640/1‑215 (MQ=255)                                                                                                                                                                                                          tggtgAATTTTTGTGAGCCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACAtt                                                            <  2:199640/215‑1 (MQ=255)                                                                                                                                                                                                                  ttttggtagtcactcaaaagatgCATCTTGAACCTCCCCACGTAATATGGCCACCGGC‑CTAA‑CGAATGTTCTTGTTTTCAAATTGTCCCGGACTTATCCTGCCCCACCATCTGTGCCGCCTCCACCGATTGTAATTACATTCGATATAATTAAACTCCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCGCGCATGTATTATc                 <  2:3117/228‑1 (MQ=2)                                                                                                                                                                                                                          gTCACCATAAAGCGGAATTTTGATCCTACCCACGTAATATGGCCACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAATAAGCTTTCCACGCAGGCATTATCGTAgcagca        <  1:1321/251‑1 (MQ=255)                                                                                                                                                                                                                                aTAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGCGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTACACACCGTTGCCCGCATTATTTCCCGGCTGATCAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCttt  >  2:316137/1‑251 (MQ=25)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               TAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGC‑CTAAGCGA‑GGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTT  >  NC_000913/1094008‑1094481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.