Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	59,092	(15-bp)2→1	coding (619‑633/651 nt)	yabP →	putative uncharacterized protein YabP

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	59090 =	1 (0.030)	8 (0.270)	8/452	1.3	89.2%	coding (617/651 nt)	yabP	putative uncharacterized protein YabP
	?	NC_000913	= 59074	1 (0.030)					coding (601/651 nt)	yabP	putative uncharacterized protein YabP

AAATCATCCCACGCTACTGCTAAGGTTGAAGGGGCGTTCCAGCCCCTTCAACCTTAGCTTATTCTGGCTCATTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/59337‑59090 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggttagcaccgtccgggGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCAACGCCCAAGTTACCTTGGCCCTTATGCAGCTGCAACGTCTGAGTAC  <  NC_000913/59074‑58886                                                                                                                                                                                                                                                                                                                                                                                                                                                       AAATCATCCCACGCTACTGCTAAGGTTGAAGGGGCGTTCCAGCCCCTTCAACCTTAGCTTATTCTGGCTCATTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTA                                                                                                                                                                                            <  1:122462/251‑1                                                                                                TTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTA                                                                                              <  1:57824/250‑1                                                                                                                        GCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGT                                                                                                                                                                              <  1:257510/146‑1                                                                                                                        GCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGT                                                                                                                                                                              >  2:257510/1‑146                                                                                                                             TTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGTAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGAAACAATCCCTCATGTCAACGCCGGGAAGGT                                                                 >  2:195585/1‑250                                                                                                                                  CAGTAGCAATTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAACTACTCCATCATATAAGGAGGTAGCACTGTTCACGCTAGAACCTTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCAGGTGTCGCACGCCAAGGCTAGCATTCCACCCAGGTCTCTCATAAAGCATATCATTGCTACCTTTTATTGTTTTTTTACCAAGGTAACAATCACTCATGTCAACGCCGGGAAGGAAAGCCT                                                           >  2:243123/1‑251                                                                                                                                                               TTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCAACGCCCAAGTTACCTTGG                              >  1:50454/1‑251                                                                                                                                                                                            CTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCAACGCCCAAGTTACCTTGGCCCTTATGCAGCTGCAACGTCTGAGaac  >  1:305942/1‑247                                                                                                                                                                                                                                                                                                                                                                                                                                                       AAATCATCCCACGCTACTGCTAAGGTTGAAGGGGCGTTCCAGCCCCTTCAACCTTAGCTTATTCTGGCTCATTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/59337‑59090 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggttagcaccgtccgggGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCAACGCCCAAGTTACCTTGGCCCTTATGCAGCTGCAACGTCTGAGTAC  <  NC_000913/59074‑58886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.