Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,549	Δ1 bp	coding (179/606 nt)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,549	0	G	.	100.0%	266.1 / NA	59	coding (179/606 nt)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (24/35);  total (24/35)

CGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACT  >  NC_000913/3207306‑3207797                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         cgcgGCACGCTGCGGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATc                                                                                                                                                                                                                                                   <  1:85104/250‑1 (MQ=255)  gcggcccgtggctgGTTAAGGCCGATCTCCCCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGt                                                                                                                                                                                                                                                 <  2:259151/241‑1 (MQ=255)     gCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGCCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTgcg                                                                                                                                                                                                                                              >  2:50971/1‑251 (MQ=255)     gCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTgcg                                                                                                                                                                                                                                              >  1:309445/1‑251 (MQ=255)          cggctgGTTCATGCCTATCTCACCGTTGAAACTCTTGCTCACACCCGAAGCGCGTTATGAAAAATATCCTTACAACCCCGATCAAAGCTGAAGATTTGCAAGTTATTCGCGTCGGCGATGTTATCTCCCTGACCGTTCCTCTGGTGTCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaaaa                                                                                                                                                                                                                                         <  2:12771/249‑1 (MQ=255)           tgctgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCATGCGCaaaa                                                                                                                                                                                                                                         >  2:257879/1‑250 (MQ=255)               ggTTCATGCCGCTCTCACCTTTGAAAATCTGTCTCACACCCGGCGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGc                                                                                                                                                                                                                                    >  1:279938/1‑251 (MQ=255)               ggTTCATGCCGATCTCACCTTTTAAAATCTGTCTCACTCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATTTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACgg                                                                                                                                                                                                                                     <  1:19767/250‑1 (MQ=255)                        cGATCTCACCTTTGAAACTTTGAATCCCACCTGGAGCGCGTTATGACAAAGATCCTGACCAACCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGAGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAACCGTCCGATCCCTTACGATCTCAACGGCAAGGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACaatagg                                                                                                                                                                                                                           >  2:295638/1‑247 (MQ=255)                              ccccgttGAAAAGCTGTCCCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCTGCGATGTGATCTACCGGCCCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTGCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATg                                                                                                                                                                                                                     <  2:297199/246‑1 (MQ=255)                              cACCTTTGAAAATCTGTCTCACCCCCGGAGCGCGTTATGAAAAAGATCCTGACACCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATggg                                                                                                                                                                                                                          <  1:155622/246‑1 (MQ=255)                                ccTTTGAAAATCTGTCTCACACCCGGAGCGCTTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATGCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGt                                                                                                                                                                                                                   <  2:309445/251‑1 (MQ=255)                                       aaaTCTGTCCTACTCCCGGAGCGCGTTATGAAAAATATCCTTCCACCCCCGATTAAACCTGAATATCTGCAAGATATTCGCGTCGGCGTTGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACTTTTGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTc                                                                                                                                                                                                            <  1:226348/251‑1 (MQ=255)                                                    acCCGGAGCGCTTTATGAAAAAGATCCTGACACCCCCGAGCAAATCTGAAGATCTGCAAGATATTCGCGTCGGCGAGGTGATCTACCTGACCGGTACTCTGTTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTAAGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCa                                                                                                                                                                                               <  1:225287/251‑1 (MQ=255)                                                      ccGGAGCGCGTGATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATTTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGt                                                                                                                                                                                             <  1:120074/251‑1 (MQ=255)                                                        ggAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAt                                                                                                                                                                                           >  1:49092/1‑251 (MQ=255)                                                           gcgcGTGATAAAAAAGATCCTGACAACCCCGATCAAAGATGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCGGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGAAAAATGGGAGATGGTCTCTGTCGGCCCGACAACCAGTATGCg                                                                                                                                                                                        <  2:201093/251‑1 (MQ=255)                                                                      aaaaGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGtt                                                                                                                                                                             <  1:50971/251‑1 (MQ=255)                                                                          tccgttcccAACCCCGATCAAAGCTGAAGATCTGCAAGAGATTCGCGCCGGCGATGTGACCTCCCTGTCCGGTACGCTGGAGACCTGCCGCCAGGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGACCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTT‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTg                                                                                                                                                                          <  2:199948/242‑1 (MQ=255)                                                                             ccTGACACCCCCGATCAAAGCTGGAGATCTGCAAGATTTGCGCTTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAACCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAAc                                                                                                                                                                       <  1:215409/250‑1 (MQ=255)                                                                                  cAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGagatcgaa                                                                                                                                                                  >  1:199948/1‑243 (MQ=255)                                                                                   aaCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAAtt                                                                                                                                                                >  1:139998/1‑251 (MQ=255)                                                                                     ccccGATCAAAGCTGAATATCTGCAAGATATTCGCGTCGGCGATGGGATCTCCCGGACCGGTACGCTGGTTACCTGCCGCGACGTTGGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCTCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGGAGGCGTATGGAAATTTTTGAACGTGAATTTa                                                                                                                                                              <  1:275468/251‑1 (MQ=255)                                                                                          aTCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGAGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTg                                                                                                                                                           >  1:169162/1‑249 (MQ=255)                                                                                                gcggAAGATCTGCAAGATATGCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACTTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGAcc                                                                                                                                                   <  1:224985/248‑1 (MQ=255)                                                                                                  tGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGa                                                                                                                                                                   >  2:27125/1‑233 (MQ=255)                                                                                                  tGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGa                                                                                                                                                                   <  1:27125/233‑1 (MQ=255)                                                                                                          ggcgaGATATGCGCGGCGGCGATGTGATCCACCTAACCGGTACGCTGTTGACCTGCCGCGACGTTGGTACCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCAGCGTGCGCAAAAACGGCGACAAATGGGAGATGGGCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGATCGTGAATTTATTGAGCAGACGGGCGTGaa                                                                                                                                           <  2:279938/245‑1 (MQ=255)                                                                                                          ggCAAGATATTCCCGTCGGGGATGGGTTCTCCCTGACCGGTACGTTGGTGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAAc                                                                                                                                         <  2:318981/250‑1 (MQ=255)                                                                                                           gCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGAACGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGCGATGGTCTCCGTCGGCCCGACAACCAGAATGAGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGaaagt                                                                                                                                        >  1:21999/1‑249 (MQ=255)                                                                                                            cAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTTTTGAGCAGACCGGCGTGAAACtg                                                                                                                                       >  2:124360/1‑251 (MQ=255)                                                                                                                  atgcggggcgccgttgtgATCTACCGGACCGGTACGCTGGTGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCAGCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtggtggg                                                                                                                                  <  2:82172/236‑2 (MQ=255)                                                                                                                        gTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaa                                                                                                                            >  1:100538/1‑250 (MQ=255)                                                                                                                           ggCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggg                                                                                                                        >  1:11815/1‑250 (MQ=255)                                                                                                                                   gATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATgg                                                                                                                 <  1:216735/250‑1 (MQ=255)                                                                                                                                     tctcccTGCCCGGTACGCTGGTAACCTGCCTCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAAc                                                                                                                                                                                                                                       <  2:215021/126‑1 (MQ=255)                                                                                                                                     tCTACATGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCACCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAAc                                                                                                                                                                                                                                       >  1:215021/1‑130 (MQ=255)                                                                                                                                        tccTGACGGGTACGCGGGTGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGc                                                                                                              <  1:256104/247‑1 (MQ=255)                                                                                                                                             gccGGTCCGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGGCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAcc                                                                                                      <  1:204044/250‑1 (MQ=255)                                                                                                                                             aCCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAcc                                                                                                      >  1:217195/1‑251 (MQ=255)                                                                                                                                                     ccTGGTGACCTGCCCGGGCGTTTGTCACCGCCGTTGGATCGTACTGAAACGGCCGTTCCCTTACTATCTCAACGGCAAAGCGATTGTCCACGCTG‑CCCCAGCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGTGTATGGAAAGTTTTGAACGTGAAGTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAgg                                                                                              <  1:140692/250‑1 (MQ=255)                                                                                                                                                                gCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAAt                                                                                   <  2:253524/251‑1 (MQ=255)                                                                                                                                                                gCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGGCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCTGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGaa                                                                                     <  1:198456/249‑1 (MQ=255)                                                                                                                                                                               aCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtg                                                                    <  1:78567/251‑1 (MQ=255)                                                                                                                                                                                        tGATCGAACTGAAACGTACGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTcc                                                           >  1:89478/1‑251 (MQ=255)                                                                                                                                                                                            cGAACTGACACGTCCGATCCCTTACGATCCCAACGGCAAAGCGATTTTCCACGCTG‑CACCATCGTGCGCAAAAACGGCGACAAATGGACGATGGTCTCCGTCGGCCCGACCACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTAAGCAGACCGGCGTGAGACTGGTGGTTGGCAACGATGGTATGGGGCCGCTGAACGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAGGGGATTTTCCCGGc                                                       >  2:321119/1‑251 (MQ=255)                                                                                                                                                                                                    atcGTCCGATCCCTTACGATCGCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTGTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTgcg                                               <  2:139998/249‑1 (MQ=255)                                                                                                                                                                                                      cGTCCGATCCCCTCCGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CACCATCGTGCGCAAAAGCGGCGACAAATGGGAGCTGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGGGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACCTGTGATTTTCCCGGCAGGCTGcgcg                                             >  2:28233/1‑251 (MQ=255)                                                                                                                                                                                                              cccTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTggc                                     >  2:290492/1‑251 (MQ=255)                                                                                                                                                                                                                   aCGATCTCAACGGCAAAGCTATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCTACAAATGTGAGATGGTCTCCGTCTGCCCGACATCCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCaa                                <  1:54888/251‑1 (MQ=255)                                                                                                                                                                                                                       tctcAACGGCACAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAg                                                                                                                          >  2:28332/1‑157 (MQ=255)                                                                                                                                                                                                                       tctcAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAg                                                                                                                          <  1:28332/157‑1 (MQ=255)                                                                                                                                                                                                                          cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTTTCCGTCGGCCCGACAACCAGTATGCGGATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTg                                                                                                                                             <  1:214586/135‑1 (MQ=255)                                                                                                                                                                                                                          cAACGGCAAAGCGATTTTCCACGCTG‑ACCCATCGTGCGCAAAAACGGCGGCAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTg                                                                                                                                             >  2:214586/1‑135 (MQ=255)                                                                                                                                                                                                                               gCAAAGCGATTTTCCACGCTG‑CCCCATCGGGCGCAAAAACGGCGACAAATGGTAGATGTTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGTCAAAGGTGGTATGGTGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCGGTCGGCAACCCAGGTGGAag                    <  2:211007/251‑1 (MQ=255)                                                                                                                                                                                                                                      gATTTTCCACGCTG‑CCCCATAGTGCGCAAAAACAGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAAAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCGGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTg              >  1:118376/1‑250 (MQ=255)                                                                                                                                                                                                                                            ccACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGAAGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAGGTTTTGAACGTGACTTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTCTGAGGCCGCTGACCGAAGAAGGCTGACAGAAATTCAAGGCGCTACATGTGATTTTCCCGGAAGGCTGCGCGGTGCTGGCGGCAAACCAGGTGGAAGAGATTgaaga          >  1:307174/1‑248 (MQ=255)                                                                                                                                                                                                                                             cACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCTGCAACCCAGGTGGAAGAGATTGAATAAGTg      <  1:239028/251‑1 (MQ=255)                                                                                                                                                                                                                                             cACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTg      <  2:217195/251‑1 (MQ=255)                                                                                                                                                                                                                                                    gCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACt  <  2:332683/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         CGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACT  >  NC_000913/3207306‑3207797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑