Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,123,823	2 bp→AG	coding (231‑232/648 nt)	grxB ←	reduced glutaredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,123,823	0	G	A	100.0%	107.9 / NA	32	L78L (CTG→TTG)	grxB	reduced glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/18);  total (14/18)
*	NC_000913	1,123,824	0	T	G	96.9%	109.7 / NA	32	L77F (TTA→TTC)	grxB	reduced glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (14/17);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCC  >  NC_000913/1123583‑1124065                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                  tCAGTTTGGCCAGCTCACGTAAATCATCTCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGTCCTCTTTCTTGTCTACGAAATATTTGCGCGCGGCGTGATTAGAAAACGCATCAAATGCCGATTGGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCGGGGGAACGTTTGCCGGTCAATAACGGttt                                                                                                                                                                                                                                           <  1:179031/250‑1 (MQ=255)       tGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTc                                                                                                                                                                                                                                     <  1:68622/250‑1 (MQ=255)       tGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGCGGGCCAGCAGGTCGGCAAAATTACACGAGCTGGACTCTTTCTTGTCGACGAACTATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCg                                                                                                                                                                                                                                    >  1:339584/1‑251 (MQ=255)             gcgcACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTa                                                                                                                                                                                                                              <  1:4350/251‑1 (MQ=255)               gcACGTACATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGCACGGTTTGCCGTCGAGTTTATc                                                                                                                                                                                                                            >  2:286979/1‑251 (MQ=255)                cACGTAAATCATCGATGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAAAGGTTTGCCGTCGAGTTTATCg                                                                                                                                                                                                                           >  1:128475/1‑251 (MQ=255)                 aCGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCg                                                                                                                                                                                                                           >  2:163494/1‑250 (MQ=255)                                 atatTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACCTAGTgcaagattgc                                                                                                                                                                                                          >  2:297739/1‑242 (MQ=255)                                             agcccGTCAGAGTGGGCCAGCATGTGGGCAAAAGTACCCGAGCTGGCCTCTTTCTTGTCGACGAAATATGTGCGCGCGGCGGGATGAGAAAACTCATCAAATGCCGATTTGGCAAAACGCCGCAACAGCTGTTTGTTGGCTTAGCCATTGCCCTTGCGCAGCCACTCTGCAATGGCAGGGGATCGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGTTGTCCATGCTTTCTgg                                                                                                                                                                                              <  2:450143/248‑1 (MQ=255)                                                  gTCAGAGTGGGCCGGCAGGTCGGCAAAAGTACCCGCGCTGGCCTTTTCCTGGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTAATCAAATGCCGATTTGGCAAAACGCGGAAACAGAGGTTTGTGGGCGTAGCCATTGCCCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCatat                                                                                                                                                                                         <  2:196989/251‑1 (MQ=255)                                                    cAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAg                                                                                                                                                                                       <  1:456028/251‑1 (MQ=255)                                                       agTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGGACGTTTGCCGGGCAAGAACGGGTTGCCGTCGAGTTTAACGACCTAGTGAACGATGTCCCTGCTTTCTGGCataaagggg                                                                                                                                                                                    >  2:419177/1‑245 (MQ=255)                                                              cagcagGTCGGCAAAATTACCCGCGCTGGCCTTTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTGGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCAt                                                                                                                                                                             <  2:400309/251‑1 (MQ=255)                                                                   ggTCGGCAAAATTACCCGCGCGGGCCTCTTTCTTGTCGACGAAATATCTGCGCGCGGCGGGAGTAGAAACCTCAACAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTGGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTAt                                                                                                                                                                                                                             <  1:323118/198‑1 (MQ=255)                                                                            aaTTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACCTAGTGCACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATg                                                                                                                                                               >  2:197640/1‑251 (MQ=255)                                                                                aCCCGCGATGGCCTCTTTCTTGTCGACGAGATATTTGCGCGCGGCGGGAGTGGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGAGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATCGTGAACGGTGTCCATGCTTTCTGGCCTGTAGCGGCTATCATCTTTTTGTAGAATGGGa                                                                                                                                                            >  2:28047/1‑250 (MQ=255)                                                                                        tGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTTCAGAAAGGGAACCTGtttt                                                                                                                                                   >  2:64155/1‑251 (MQ=255)                                                                                                                             gggAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGcgtcgtcgt                                                                                                              >  1:114814/1‑251 (MQ=255)                                                                                                                                   aGAAAACTCATCAACTGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGTCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGACAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGCACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGGGTTTCTGCGTCGTCGTTGAGc                                                                                                         >  2:215933/1‑250 (MQ=255)                                                                                                                                      aaaCTCATCAAATGCCGATTTGGCAAAACGCGTCCACAGCAGTTTGTTGGCGTAGCCATTGCCCTTGCGCAGTCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGGCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGTCCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGaa                                                                                                     <  1:353743/251‑1 (MQ=255)                                                                                                                                               aaaTGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTGCTGCGTCGTCGTTGAGCAGAACATGTAAtt                                                                                            <  1:215933/251‑1 (MQ=255)                                                                                                                                                     cGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTAAATGGCAGGGGAACGTTTGCCGTTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCCTATAGCGGCTGTCATCTTTTTGCAGACTGGGAACCTGTTTTTTCCCGACCATCCGGGGGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACgg                                                                                      >  2:143171/1‑251 (MQ=255)                                                                                                                                                              aaaaCGCGGCAACAGAAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATAtttt                                                                             <  2:534655/251‑1 (MQ=255)                                                                                                                                                                            gcattttGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATc                                                               <  1:119859/247‑1 (MQ=255)                                                                                                                                                                                              cATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAg                                             <  1:163494/251‑1 (MQ=255)                                                                                                                                                                                                   aCCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAgg                                        <  1:158484/251‑1 (MQ=255)                                                                                                                                                                                                       tGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGc                                      <  1:5125/249‑1 (MQ=255)                                                                                                                                                                                                        gcgcAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGt                                   <  1:419177/251‑1 (MQ=255)                                                                                                                                                                                                          gcAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGa                                 <  2:114814/251‑1 (MQ=255)                                                                                                                                                                                                              ccACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCCTCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGt                             >  2:480407/1‑251 (MQ=255)                                                                                                                                                                                                                                    cGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGAct       <  1:64155/251‑1 (MQ=255)                                                                                                                                                                                                                                         gCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACtcctcc  >  2:216152/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                  TCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCC  >  NC_000913/1123583‑1124065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑