Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,038,477	2 bp→AA	coding (738‑739/1545 nt)	appC →	cytochrome bd‑II subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,038,477	0	G	A	100.0%	163.6 / NA	47	A246A (GCG→GCA)	appC	cytochrome bd‑II subunit 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/21);  total (26/21)
*	NC_000913	1,038,478	0	C	A	100.0%	171.1 / NA	47	Q247K (CAA→AAA)	appC	cytochrome bd‑II subunit 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/21);  total (26/21)

GAGATGACCAGCTTCAGCGAGCTGGTCTTTAATCCGGTCAGCCAGGTGAAATTTGTGCACACCGTAATGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCGCAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAATTTGATGGCTGAAACCTACCCACGCTTGCAACGCGGACGTATGGCCTGG  >  NC_000913/1038238‑1038714                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                             gagaTGACCAGCTTCAGCGAGCTGGTCTTTAATCCGGTCAGCCAGGTGAAATTTGTGCACACCGTAAAGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCAGCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAAcc                                                                                                                                                                                                                                    <  1:530303/251‑1 (MQ=255)               cAGCGAGCTGGTCTTTAATCCGGTCAGCCAGGTGAAATTTGTGCACACCGTAATGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGTCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTggcg                                                                                                                                                                                                                       <  1:190875/250‑1 (MQ=255)               cAGCGAGCTGGTCTTTAATCCGGTCAGCCAGGTGAAATTTGTGCACACCGTAATGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTggcg                                                                                                                                                                                                                       <  1:469365/250‑1 (MQ=255)                   gAGCTGGTCTTTAATCCGGTCAGCCAGGTGAAATTTGTGCACACCGTAATGGCGGGCTTCGTGACCGGGGCCCTGTTTATTATGGCGATCAGCGCCTGGGATTTACTGCGCGGACGGGAGCGCAATGTCGCTTTACCCTCGTTTGCCATCGGGTCCGTCTTCGGTCCTCTGGCGATTATAGGGACCCTGCAACCCAGAGACAGTTTTGCGTATGAAGTCGCAAAAGGACAACCGGGAAAACTggggggg                                                                                                                                                                                                                    >  2:301829/1‑244 (MQ=255)                             ttAATCCGGTCAGCCAGGTGAAATTTGTGCACACCGTAATGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAgggg                                                                                                                                                                                                          <  2:151781/249‑1 (MQ=255)                               aaTCCGGTCAGCCAGGTGAAATTTGTGCACTCCGTAATGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTg                                                                                                                                                                                                      <  1:383800/251‑1 (MQ=255)                                        aGCCAGGTGAAATTTGTGCACACCGTAATGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGGATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATTAAGTCGCAAAAGTACAACCGGTAAAAATGGCGGCGGTGGCAGGGGAGTGGCaaaagg                                                                                                                                                                                              >  1:141007/1‑247 (MQ=255)                                                 aaaTTTGTGCACACCGTAATGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAacctgcacc                                                                                                                                                                                    <  2:182433/251‑1 (MQ=255)                                                               cGTAATGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACTTGCACCGTTCCAtg                                                                                                                                                                      <  2:409574/251‑1 (MQ=255)                                                                        gggCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCt                                                                                                                                                             <  1:194038/251‑1 (MQ=255)                                                                         ggCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTg                                                                                                                                                            <  1:222384/251‑1 (MQ=255)                                                                          gCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTgg                                                                                                                                                           >  2:92592/1‑251 (MQ=255)                                                                            tACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTAAGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGCCAGTTCTGCGTATGAAGTCGCAAAAGTCCAACCGGTAAAACTGGCGGCGATGGAGGGGGAGGGCCAAACGGAACCTGCACCTGCCCCGTTCCATGTGGTTGCCTGGcc                                                                                                                                                         >  2:523504/1‑251 (MQ=255)                                                                               gTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCgg                                                                                                                                                       >  1:160857/1‑250 (MQ=255)                                                                                tGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGa                                                                                                                                                      >  2:472903/1‑250 (MQ=255)                                                                                tGACCGGGGCCATGTTTATTATGGCGATCAGCGACTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGGACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGa                                                                                                                                                      >  2:402922/1‑250 (MQ=255)                                                                                 gACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAAc                                                                                                                                                    >  2:193923/1‑251 (MQ=255)                                                                                        gCCATGATTATTATGGCGATCAGCGCCTGGCATTTACTGCGCGGACGGGAGCGCCATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGCCAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATc                                                                                                                                              >  2:379805/1‑250 (MQ=255)                                                                                                                    ggTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCaaaa                                                                                                                   <  1:147123/249‑1 (MQ=255)                                                                                                                       aTTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTGTGCCCTCAAAATTccc                                                                                                              <  2:252242/251‑1 (MQ=255)                                                                                                                             tGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCgctg                                                                                                        >  1:182285/1‑251 (MQ=255)                                                                                                                                 cgGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATGATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAg                                                                                                    <  1:379805/251‑1 (MQ=255)                                                                                                                                  ggACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTTCTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAgg                                                                                                   <  2:160857/251‑1 (MQ=255)                                                                                                                                      gggAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATc                                                                                               <  1:380297/251‑1 (MQ=255)                                                                                                                                      gggAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATc                                                                                               >  1:464951/1‑251 (MQ=255)                                                                                                                                            gcAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGcc                                                                                         >  1:231630/1‑251 (MQ=255)                                                                                                                                                tGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGAAAACGGAACCTGCACCTGCAACGTTCCATGTGGTTGCCTGGCCGGCACAGGATCAAGAGCGTAAAGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCccctc                                                                                     >  2:404811/1‑251 (MQ=255)                                                                                                                                                     cATTACGCTCGTTTGCCATTGGTTCCGTCTTCGTTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAACCTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGGTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCactcactc                                                                                 >  2:531704/1‑250 (MQ=255)                                                                                                                                                      aTTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCAt                                                                               >  1:446353/1‑251 (MQ=255)                                                                                                                                                                    ccATCGGTTCCGTCTTCGGTTCTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGTAACCTGCGCCTGCACCGTTCCATGTGGTGGCCTGGCGGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTg                                                                 <  1:541890/251‑1 (MQ=255)                                                                                                                                                                         ggTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGt                                                                  <  1:281359/245‑1 (MQ=255)                                                                                                                                                                         ggTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGt                                                                  >  2:281359/1‑245 (MQ=255)                                                                                                                                                                            tCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCt                                                         >  1:112352/1‑251 (MQ=255)                                                                                                                                                                                   tCGGTACTCTGGCGATTAACGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCATGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCTTGCCGGGTCTgaagaa                                                   >  1:290493/1‑250 (MQ=255)                                                                                                                                                                                    cGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTACTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAAtt                                                 <  1:761/251‑1 (MQ=255)                                                                                                                                                                                    cGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTACTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAAtt                                                 >  2:761/1‑251 (MQ=255)                                                                                                                                                                                     ggTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAAttt                                                >  1:463827/1‑251 (MQ=255)                                                                                                                                                                                         ctctGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGACGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCCTGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAACTTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAATTTGATg                                            >  2:467069/1‑251 (MQ=255)                                                                                                                                                                                                   tATCGGTACCCTGCAACCCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGTCGGCGATGGAAGGTGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTTGCCGTAACAGGATCAAGAGCGTAACTCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCAGTAGATAAACCCGTGCCGGGTCTGAATAATTTGATTTCTGAAAcc                                   <  1:193923/250‑1 (MQ=255)                                                                                                                                                                                                    aTCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAATTTGATGGCTGAAACCt                                  >  1:472351/1‑250 (MQ=255)                                                                                                                                                                                                            ccTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAATTTGATGGCTGAAACCTACCCACGCt                         >  1:94941/1‑251 (MQ=255)                                                                                                                                                                                                                     cGGAGACAGCTCTGAGTACGAAGTCGCAACAGCCCAACCGGCAAAAATGGCGGCGATGGAAGGGGAGTAGCAAACGGAACCTGCACCGGCACCGTTCCATGTGGTTGCCTGGCAGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAATTTGATGGCTGAAACCTACCCACGCTTGCAAcgcg                >  1:439582/1‑251 (MQ=255)                                                                                                                                                                                                                      ggAGACAGATCTGCGTATGAAGTCGCAACAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTCGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGACGAATTTGATGGCTGAAACCTACCCACGCTTGCAACGCgg               >  2:484520/1‑251 (MQ=255)                                                                                                                                                                                                                         gaCAGTTCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAATTTGATGGCTGAAACCTACCCACGCTTGCAACGCGGACg            <  2:155016/251‑1 (MQ=255)                                                                                                                                                                                                                               tCTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAATTTGATGGCTGAAACCTACCCACGCTTGCAACGCGGACGTATgg       <  2:123265/250‑1 (MQ=255)                                                                                                                                                                                                                                cTGCGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAATTTGATGGCTGAAACCTACCCACGCTTGCAACGCGGACGTATGGcc     >  2:249170/1‑251 (MQ=255)                                                                                                                                                                                                                                   cGTATGAAGTCGCAAAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAAGTTGATGGCTGAAACCTACCCACGCTTGCAACGCTGACGTATGGCCTgg  <  2:231630/251‑1 (MQ=255)                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                             GAGATGACCAGCTTCAGCGAGCTGGTCTTTAATCCGGTCAGCCAGGTGAAATTTGTGCACACCGTAATGGCGGGCTACGTGACCGGGGCCATGTTTATTATGGCGATCAGCGCCTGGTATTTACTGCGCGGACGGGAGCGCAATGTCGCATTACGCTCGTTTGCCATCGGTTCCGTCTTCGGTACTCTGGCGATTATCGGTACCCTGCAACTCGGAGACAGTTCTGCGTATGAAGTCGCGCAAGTACAACCGGTAAAACTGGCGGCGATGGAAGGGGAGTGGCAAACGGAACCTGCACCTGCACCGTTCCATGTGGTTGCCTGGCCGGAACAGGATCAAGAGCGTAACGCCTTTGCCCTCAAAATTCCCGCGCTGCTAGGGATCCTCGCCACTCACTCATTAGATAAACCCGTGCCGGGTCTGAAGAATTTGATGGCTGAAACCTACCCACGCTTGCAACGCGGACGTATGGCCTGG  >  NC_000913/1038238‑1038714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑