Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,555,047 2 bp→TG coding (1963‑1964/2706 nt) malT → DNA‑binding transcriptional activator MalT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,555,0470GT98.6% 223.0 / NA 70V655C (GTC→TGC) malTDNA‑binding transcriptional activator MalT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (36/33);  minor base A (1/0);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
*NC_0009133,555,0480TG98.5% 217.1 / NA 70V655C (GTC→TGC) malTDNA‑binding transcriptional activator MalT
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (34/33);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GCATTCGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCTTA  >  NC_000913/3554805‑3555287
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                               
gcATTCGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAAGGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTAAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAGCAAATGCCGGGTg                                                                                                                                                                                                                                          <  2:75249/251‑1 (MQ=255)
  aTTCGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGAt                                                                                                                                                                                                                                        >  2:97903/1‑251 (MQ=255)
  aTTCGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGAt                                                                                                                                                                                                                                        >  1:134688/1‑251 (MQ=255)
   ttCGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGCCTGGATCTCTAACGCCAACAAATGCCGGGTGAtt                                                                                                                                                                                                                                       >  2:157841/1‑251 (MQ=255)
     cGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGAttt                                                                                                                                                                                                                                      <  1:45201/250‑1 (MQ=255)
          gcAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGc                                                                                                                                                                                                                                >  1:151160/1‑251 (MQ=255)
          gcAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGc                                                                                                                                                                                                                                >  1:83654/1‑251 (MQ=255)
                gTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATg                                                                                                                                                                                                                           >  1:536113/1‑250 (MQ=255)
                   aTGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCg                                                                                                                                                                                                                       >  2:481061/1‑251 (MQ=255)
                   aTGGGCCTGGGCGCGGCTGGATGAAGACGAAGCGGCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTAACAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCg                                                                                                                                                                                                                       <  2:11003/251‑1 (MQ=255)
                     gggCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGAGCCTGGCAATGTTGATTCAATGCTCTCTGGCCCGTGGTGATTTAGATAACGCCCGTATCCATCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAAGGACCGGc                                                                                                                                                                                                                     <  2:351627/251‑1 (MQ=255)
                                cgGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAgccgc                                                                                                                                                                                                          >  2:150992/1‑251 (MQ=255)
                                 ggCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAgccgcc                                                                                                                                                                                                         <  2:277069/251‑1 (MQ=255)
                                   cTGGATGAAGCCGCAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCCATGTTGATTCAATGCTCGCTGGCCCGTGGCGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATACCGGGTGATTTAATGGCAAATGACCGGCGATAAAGCcgccgc                                                                                                                                                                                                       >  1:462753/1‑251 (MQ=255)
                                     ggATGAAGCCGAAGCGTCGGCGCTTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGTTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGTTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCTGCGATTAAGCcgccgc                                                                                                                                                                                                       <  1:477214/249‑1 (MQ=255)
                                     ggATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGGCTTAACCGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTg                                                                                                                                                                                                     <  2:425903/251‑1 (MQ=255)
                                      gATGAAGCCGAAGCGTAGGCGCGTAGCGGTGTTGAAGTCTTGTCGTCTTCTCAGCCACAGCACCAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGACAACCTGCTGGGGAATGGCAAATATCACCGCGACTGGATCTCTAACGCCAGAACATGCCGGGTGATTTACTGGAAAATGACAGGCGATACAGCCGCCGCTgg                                                                                                                                                                                                    >  2:370451/1‑250 (MQ=255)
                                        tGAAGCCGAAGGGTCGGCGCGTAGAGGAATTGAAGTCATGTCGTCTTATCAGCCACAACACCAGCTTCAGTGACTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGAGACTGGATCTCTAACGCCAACAAATCCCGGGTGATTTACTGGCAAATGACAGGCGATAAAGCCGCCGCTGcc                                                                                                                                                                                                   >  2:325261/1‑250 (MQ=255)
                                                aaGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTg                                                                                                                                                                                          >  1:98789/1‑251 (MQ=255)
                                                 aGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATAGCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGc                                                                                                                                                                                         <  1:200036/251‑1 (MQ=255)
                                                    gTCGGCGCGTAGAGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTc                                                                                                                                                                                      >  1:137635/1‑251 (MQ=255)
                                                            gTAGCGGGCTTGAAGGCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGACAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCACTGCCAACTGGTTGCGTCATACGGCt                                                                                                                                                                              >  2:344112/1‑251 (MQ=255)
                                                                        aaGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCGCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGttt                                                                                                                                                                  <  2:443093/251‑1 (MQ=255)
                                                                                      tATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACtt                                                                                                                                                    >  1:399266/1‑251 (MQ=255)
                                                                                      tATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACtt                                                                                                                                                    >  1:484994/1‑251 (MQ=255)
                                                                                      tATCAGCCACAGCAACAGCTTCAGTGCCTGGAAATGTTGATTCAAGGCTTGCGGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAATCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCa                                                                                                                                                       <  1:368457/248‑1 (MQ=255)
                                                                                          aGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTg                                                                                                                                                <  1:205368/251‑1 (MQ=255)
                                                                                             cacaGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGTTGATTTACTGGCAAAGGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTGCCTGCaa                                                                                                                                             <  2:236889/251‑1 (MQ=255)
                                                                                              acaGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCa                                                                                                                                              >  1:495583/1‑249 (MQ=255)
                                                                                               caGCAACAGCGTCAGTGCCTGGCTATGTTGATGCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCGGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAAGGACCGGCGATAAAGCCGCCGCTGCCAATTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTTCAAgg                                                                                                                                           <  2:486186/251‑1 (MQ=255)
                                                                                                aGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGt                                                                                                                                          <  1:377294/251‑1 (MQ=255)
                                                                                                aGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGt                                                                                                                                          >  2:156651/1‑251 (MQ=255)
                                                                                                       gCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTTGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGTCTGGATCGCTAACGCCAACAAATGCCGGGTGATTTACTGTCAATTGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTGTGCGAACAACCACTTCCGGCAAGGTCAATGgc                                                                                                                                   <  2:261125/251‑1 (MQ=255)
                                                                                                             gTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAAc                                                                                                                              >  1:118093/1‑250 (MQ=255)
                                                                                                               gCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACAtt                                                                                                                           >  1:100003/1‑251 (MQ=255)
                                                                                                               gCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACAt                                                                                                                            <  1:111620/250‑1 (MQ=255)
                                                                                                                   ggCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGccc                                                                                                                       <  1:292503/251‑1 (MQ=255)
                                                                                                                     cAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGt                                                                                                                     <  2:249253/251‑1 (MQ=255)
                                                                                                                               tCAATGCTCGCTGGCCCGGGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCGCTAACGCCAACAAATGCCGGTTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACATCCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATc                                                                                                            <  1:252414/250‑1 (MQ=255)
                                                                                                                                cAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCt                                                                                                           <  2:112865/250‑1 (MQ=255)
                                                                                                                                       cgcgggcactgggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGGCTGGAAAACCTGCTGGGGAATGGCAAAAATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTgc                                                                                                     <  2:297577/238‑2 (MQ=255)
                                                                                                                                                  ggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAAcc                                                                                        <  1:425935/251‑1 (MQ=255)
                                                                                                                                                      aTTTAGAAAACGAACGTAGCCAGCACACCAGTCTGGCAAACCTGCTGGGGAATGGCAAATATCACAGCGACTCGATCTCTCACGCCAAAAAATGCCGGGTGCTTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCCATGTCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGCTTggacaggc                                                                                     >  2:38588/1‑243 (MQ=255)
                                                                                                                                                        ttAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGACGCCGCTGCCAACTGGTTACGTCATACGGCTAAACCAGAGTTTGCGAACAACACCTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCgga                                                                                     >  1:211182/1‑247 (MQ=255)
                                                                                                                                                        taagacaaCGCCCGTAGCCAGCGGAACCGTCTGGAAAACCGGCTGGGGAATGTCAAATATCACAGCGAGTGGTTGTCTAACGCCACCAAATGCCGCGTGAGTGACTTGCAAATGTCCGGCGATAAAGCCGCCGCTGCCATTTGGTTGGGTCAGACGTCGAAACCAGAGTTGGCGGTCAAGCACTGCCTGCAAGGTCAAGTGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCTAGTTTGATCCGGCAg                                                                                   <  1:362136/244‑1 (MQ=255)
                                                                                                                                                           gATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACAGTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAAt                                                                               <  1:219922/251‑1 (MQ=255)
                                                                                                                                                                    cGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCg                                                                       >  1:293421/1‑250 (MQ=255)
                                                                                                                                                                      tAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCATTGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCga                                                                      >  1:435974/1‑249 (MQ=255)
                                                                                                                                                                          cAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACt                                                                <  2:83654/251‑1 (MQ=255)
                                                                                                                                                                             cTGAACCGTCTGGACAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCACTGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCa                                                              >  2:378111/1‑250 (MQ=255)
                                                                                                                                                                               gatccGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAAGGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAAGTGTTCGCGAAGACCTCa                                                              <  2:461182/245‑1 (MQ=255)
                                                                                                                                                                               gAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATg                                                           >  1:316552/1‑251 (MQ=255)
                                                                                                                                                                                aaCCGTCTGGAACACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATg                                                           >  2:284112/1‑250 (MQ=255)
                                                                                                                                                                                 aCCGTCTGGAAAACATGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTCACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATACAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCCGAGTTTGCGACCAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGa                                                          >  2:500465/1‑250 (MQ=255)
                                                                                                                                                                                        ggAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGTCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGcc                                                   <  1:289998/250‑1 (MQ=255)
                                                                                                                                                                                           aaaCCTGCTGGGGAATGGCAAATATCACAGCGAATGGATCTCCAACGCCAACAAATGCCGGGTGATTTACGGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGa                                               <  2:435974/251‑1 (MQ=255)
                                                                                                                                                                                               ctgctgGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCt                                           >  1:124256/1‑251 (MQ=255)
                                                                                                                                                                                                  ctgGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGACGGGCGATTTAGTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCCACTGGTTGAGTCATACGGCGAAACCAGAGTTTGAGAACAACCACTTCCTGGAAGTGCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGTGTTTGAACCGGCAGAAATTGGTCTCGAAGCACTCAATGAAAATGccaggtgtatgcg                                        >  2:158263/1‑240 (MQ=255)
                                                                                                                                                                                                    ggggAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTTTGCGGt                                      <  2:462511/251‑1 (MQ=255)
                                                                                                                                                                                                      ggAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTtg                                    >  2:501290/1‑251 (MQ=255)
                                                                                                                                                                                                          tGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGTGCGAGttt                                                                                             <  2:471962/190‑1 (MQ=255)
                                                                                                                                                                                                          tGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGttt                                                                                             >  1:471962/1‑190 (MQ=255)
                                                                                                                                                                                                             aaaaTATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCg                             <  2:454752/250‑1 (MQ=255)
                                                                                                                                                                                                                     acaGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAAc                     >  1:503334/1‑251 (MQ=255)
                                                                                                                                                                                                                      caGCGACTGGATCTCTACCGCCAACAAAAGACGAGTGATTTACTGGCACGTGACCTGCGACAAAGCCGCCGCTGACACCTGGTTGCGTAATACGGCTAAACCAGAGTTTGCGCACAACCACATCATGCAAGGTCAATGGAGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAAACGGCAGACATTGTTCTCGAAGAACTCACTGCAAATGCCCGGAGTATGAGGTTGATGCGCGATCTCAAcc                    >  2:33989/1‑251 (MQ=255)
                                                                                                                                                                                                                       aGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCg                   >  1:278741/1‑251 (MQ=255)
                                                                                                                                                                                                                          gACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTa                 >  2:163965/1‑250 (MQ=255)
                                                                                                                                                                                                                              ggATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCt             <  1:500465/250‑1 (MQ=255)
                                                                                                                                                                                                                               gATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGt           >  2:238503/1‑251 (MQ=255)
                                                                                                                                                                                                                                        cGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGAGGAGCGATCTCAACCGTAACCTGTTGCTGCTTa  <  1:163965/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                               
GCATTCGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCTTA  >  NC_000913/3554805‑3555287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑