Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,002,825	C→T	G261E (GGA→GAA)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,002,825	0	C	T	100.0%	155.5 / NA	42	G261E (GGA→GAA)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/24);  total (18/24)

AGTTTTAACAGAAACAGCACCAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTCCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTG  >  NC_000913/2002578‑2003050                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  aGTTTTAACAGAAACAGCACCAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGTAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAg                                                                                                                                                                                                                                <  1:509919/251‑1 (MQ=255) aGTTTTAACAGAAACAGCACCAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAg                                                                                                                                                                                                                                <  1:227167/251‑1 (MQ=255)            aaaCAGCACCAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTc                                                                                                                                                                                                                     <  2:170267/251‑1 (MQ=255)                  cACCAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGt                                                                                                                                                                                                                >  2:200639/1‑250 (MQ=255)                   aCCAGTCGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTCTCATTACCCTTGGCATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTAc                                                                                                                                                                                                              >  2:225358/1‑251 (MQ=255)                   aCCAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTAc                                                                                                                                                                                                              <  2:92632/251‑1 (MQ=255)                   aCCAGTAGTTTCACTCACATCCGCAGCGTACAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTAACTGTAc                                                                                                                                                                                                              >  1:408186/1‑251 (MQ=255)                     cAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACATGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACca                                                                                                                                                                                                            <  1:337717/251‑1 (MQ=255)                         attttCACTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACCGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACcatca                                                                                                                                                                                                         <  2:408186/248‑1 (MQ=255)                           tttCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCAtt                                                                                                                                                                                                       >  1:484450/1‑250 (MQ=255)                            ttcattcaCATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACATTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTa                                                                                                                                                                                                      <  1:541428/250‑1 (MQ=255)                                       ccGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTAc                                                                                                                                                                                           <  1:304447/250‑1 (MQ=255)                                                    aTTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTcc                                                                                                                                                                             <  1:55901/251‑1 (MQ=255)                                                       gCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCa                                                                                                                                                                           <  1:225358/250‑1 (MQ=255)                                                        ccATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATc                                                                                                                                                                         >  1:39762/1‑251 (MQ=255)                                                            ttGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGtt                                                                                                                                                                      <  1:315885/250‑1 (MQ=255)                                                              gTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTAt                                                                                                                                                                    <  2:179120/250‑1 (MQ=255)                                                                    ttAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACGGTTACTGCGTAATACTTCCCATCGTTATcacc                                                                                                                                                                <  1:170442/248‑1 (MQ=255)                                                                           cATATGTATCGGTATCATTGCCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCAGCATTAGCAACTGGTACGTCGTAATACTTCCCAGCGTTATCTCCACCGGTGAtt                                                                                                                                                      <  2:26889/251‑1 (MQ=255)                                                                             tatGTATAGGTATCATGACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGACTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCCCTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCCACGGTGAtttt                                                                                                                                                    >  2:144368/1‑251 (MQ=255)                                                                              atGTAGCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTc                                                                                                                                                   <  1:140491/251‑1 (MQ=255)                                                                                                                         gCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTg                                                                                                        <  2:451183/251‑1 (MQ=255)                                                                                                                                gCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTgg                                                                                                 <  1:200639/251‑1 (MQ=255)                                                                                                                                  aCCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGt                                                                                               >  2:8703/1‑251 (MQ=255)                                                                                                                                         ttGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTcc                                                                                        >  2:150847/1‑251 (MQ=255)                                                                                                                                                     gAACCTGCAGTCTTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGt                                                                            >  2:78762/1‑251 (MQ=255)                                                                                                                                                      aaCCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTg                                                                           <  1:386045/251‑1 (MQ=255)                                                                                                                                                           gCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGgctgc                                                                      <  1:462213/251‑1 (MQ=255)                                                                                                                                                                   atCAATCTGAACAGGTGTACCGCCTGAAGAGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGt                                                                >  2:111606/1‑249 (MQ=255)                                                                                                                                                                       atctgacctgctgtaaatcatGAAGCGATAGTTGAAGAATTCGTAGTATTTGCATCAGTTCCAGTTGCATTTGAAGTTGCTTCAGTCGCCATTGTCACTGTAACATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTTTCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCATCAATTGCAGCTGGGTTAATTCAGCCAGTATCAGTGTCTGCTTCCGTAGaacg                                                          >  2:517992/21‑249 (MQ=255)                                                                                                                                                                             aCAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAAt                                                    >  1:216280/1‑251 (MQ=255)                                                                                                                                                                                      ccGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCAGCCAGTAt                                                                                >  2:195825/1‑214 (MQ=255)                                                                                                                                                                                      ccGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCAGCCAGTAt                                                                                <  1:195825/214‑1 (MQ=255)                                                                                                                                                                                        gCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATa                                                                                                                                              >  1:475820/1‑150 (MQ=255)                                                                                                                                                                                        gCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATa                                                                                                                                              <  2:475820/150‑1 (MQ=255)                                                                                                                                                                                                  aTAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCCTTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATAttgtt                               >  2:198413/1‑251 (MQ=255)                                                                                                                                                                                                            cTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCTCTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTggtggt                      >  2:327352/1‑250 (MQ=255)                                                                                                                                                                                                                   agtaTTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCa               <  2:216280/250‑1 (MQ=255)                                                                                                                                                                                                                   agtaTTTGCATCAGTTACAGTTGCATTTGCAGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTTTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTAGAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCaa              <  1:391410/251‑1 (MQ=255)                                                                                                                                                                                                                    gtaTTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTTTACCATCCTTAGCAACTGTTACTGCGTACTACTTCCCATCGTTATCAACACCGGTGATTTTCGCATAGTAATCATTTCCCTTATCAGTATAACCACCCTCAATTGACGCTGGGTTAGTTCCTCCAGTATCATTGGCTGCTTCCGTAGCAAGTGTAATTCCCGTATGTTTACTATTGTTTTTGGTGGTAGCACCaa              >  2:241860/1‑250 (MQ=255)                                                                                                                                                                                                                    gtaTTTGCATCAGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCaa              >  1:49996/1‑250 (MQ=255)                                                                                                                                                                                                                               aGTTACAGTTGCATTTGCCGTTGCTTCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAAGTGAAGCTGGGTTAGTTCCGCCAGGATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATGGTTTGTGGTGGTAGCACCAAAAGCAGTTACTg  <  2:39762/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  AGTTTTAACAGAAACAGCACCAGTAGTTTCATTCACATCCGCAGCGTAAAGATTGCCATTTGTATCTTTAAGCGCATATGTATCGGTATCATTACCCTTGGAATCCTGCAGTTTTACTAAGCTAACAGCACCAAGGTTGGCAGTTGCGGAACCTGCAGTATTATCAATCTGAACAGGTGTACCGCCTGAAGTGATAGTTGTAGCTTTAGTAGTATTTGCATCAGTTACAGTTGCATTTGCCGTTGCTCCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTG  >  NC_000913/2002578‑2003050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑