Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,836,397	G→A	E109K (GAG→AAG)	ynjB →	putative ABC transporter periplasmic binding protein YnjB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,836,397	0	G	A	100.0%	126.4 / NA	39	E109K (GAG→AAG)	ynjB	putative ABC transporter periplasmic binding protein YnjB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/25);  total (14/25)

AAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAGAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACC  >  NC_000913/1836152‑1836624                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     aaaaTGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGcc                                                                                                                                                                                                                                 <  1:325695/251‑1 (MQ=255)   aaTGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCaa                                                                                                                                                                                                                               <  1:45487/251‑1 (MQ=255)      gAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTAGCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTGCCGCTCCTTAAAAAAGGCCAAtt                                                                                                                                                                                                                             <  2:482349/250‑1 (MQ=255)           cAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGACAACTTCCGCACCTTAAAAAAGGCCAATTTATTAc                                                                                                                                                                                                                       >  1:461739/1‑251 (MQ=255)                  cAAACCGTCTGATTTAACGCCTGGGGCGGCGATACCGCCATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCCATTTATTACAAACggg                                                                                                                                                                                                                >  2:54892/1‑251 (MQ=255)                                 aaCGCCTGGGGCGGCGATACCGCAAGTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGActct                                                                                                                                                                                                 <  1:433455/251‑1 (MQ=255)                                     gcTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCACAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGACAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGccc                                                                                                                                                                                             >  2:21704/2‑251 (MQ=255)                                          ggcggcGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCAGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAACAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGACCAACTg                                                                                                                                                                                        >  2:294706/1‑251 (MQ=255)                                              gcgATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGgc‑gc                                                                                                                                                                                   <  1:344142/251‑1 (MQ=255)                                                          ttAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCCATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGCACGGCGAAAACTTCCGCACCTTACAAAAGGCCAATTTATTACAACCGGGATGGGCGGAGACTCTGCCCAACTGGC‑TCTATGTCggc                                                                                                                                                                          >  2:117346/1‑246 (MQ=255)                                                             aCCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGACCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGG‑CGGAGGCTCTGGCCCACTGGCCGC‑AAGTCGAcacacaac                                                                                                                                                                   >  2:205457/1‑249 (MQ=255)                                                                   aTCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACCCAGCTGCCg                                                                                                                                                              >  2:142308/1‑251 (MQ=255)                                                                   aTCTCGACTGGGAGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGCAGATTGTCCGTCTGGCGGATGACGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGAGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTCTGTCGACACACAGCTGCCg                                                                                                                                                              >  1:524108/1‑251 (MQ=255)                                                                        gACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAACCCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGTCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACATCTGCCGGTGCg                                                                                                                                                         <  2:356869/251‑1 (MQ=255)                                                                                          aTGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGAGCGGGAAGATTTTTCTGTGcc                                                                                                                                       >  2:15007/1‑251 (MQ=255)                                                                                                 cccATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACaa                                                                                                                                <  1:136271/251‑1 (MQ=255)                                                                                                           tataAACATGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGAGCGGGAAGATTTTTCTGTGCCGACACAAGGAGCGGAAt                                                                                                                      >  2:195820/1‑251 (MQ=255)                                                                                                               aaCCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGcc                                                                                                                  <  1:438458/251‑1 (MQ=255)                                                                                                                           gTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACGCAGCTGCCGGTGCGGGAAGGTTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGtcggcg                                                                                                       <  2:192481/250‑1 (MQ=255)                                                                                                                                   ggCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTg                                                                                               <  1:466609/250‑1 (MQ=255)                                                                                                                                      ggATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACCACTGACGt                                                                                           >  2:246566/1‑251 (MQ=255)                                                                                                                                         tGCCGCAGGCGCTGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAACCGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGTCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACTCACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGttt                                                                                         <  1:466674/250‑1 (MQ=255)                                                                                                                                                         aaGCGGATGCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGGTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTGTCTTTGCCGACACAAGGTGCTGAATCGCCCTGGGGCGGCGTACAACTGACGTTTATCGCCCGCCGCGATGt                                                                        <  2:492178/251‑1 (MQ=255)                                                                                                                                                         aaGCGCAGTAAGCCCGAAGCCTAAGCCGGACGTAAAACGGTCGGCTCGGTTGCTCTGCTCTGTGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTGCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCTGCGCACAACTGACGTTTATCGCCCGCCGCGATGt                                                                        <  1:490078/251‑1 (MQ=255)                                                                                                                                                              cATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCGGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGc                                                                   <  2:191818/251‑1 (MQ=255)                                                                                                                                                                 tCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCGGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCGGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCca                                                                 <  2:374798/250‑1 (MQ=255)                                                                                                                                                                                       cGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGc                                          >  1:401490/1‑251 (MQ=255)                                                                                                                                                                                          aaaaCGGGCGGCTCGGTGGATCTGCTCTGGGGGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCtt                                       <  2:384286/251‑1 (MQ=255)                                                                                                                                                                                           aaaCGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGTTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTa                                      <  1:21127/251‑1 (MQ=255)                                                                                                                                                                                                ggcgtcTCGGTGGATCTGCTCTGGGTGAACGGAGAAAACTGCCGCACCTTAAAAAAGGCCATTTTATGACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTACGGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGa                                 <  2:17905/251‑1 (MQ=255)                                                                                                                                                                                                     cTCGGTGGATCTGCTCTGGGTGAACGGCGAAACCTTCCGCACATTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCCCAACCGCCGCAAGCCTTACTGGAGTTTg                            >  2:58600/1‑251 (MQ=255)                                                                                                                                                                                                           ggATCTGCTCGGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACCCAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAg                      <  1:180199/251‑1 (MQ=255)                                                                                                                                                                                                                 gCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATc                <  2:410244/251‑1 (MQ=255)                                                                                                                                                                                                                  ccctGGGTGAACGGCGAAAACTTCCGCCCCTTAAAAAAGGCCAATTTATTACAAACGGGTTGGGCGGAGACTCTGCCCAACTGGC‑GTTATGCCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACGGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGTAGTTTGCTAAAGCCAATcc               <  2:25695/249‑1 (MQ=255)                                                                                                                                                                                                                      gggTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTGATTCAAAACGGGCTGGGCGGAGACTCTGCCCAACGGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAATATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCCCCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCgg            <  2:344745/250‑1 (MQ=255)                                                                                                                                                                                                                       ggggAACGGGGAAAACTTCCGGACCTAAAAAAAGGCAAATTTATTACAAACGGGCTGGGGGGAGACTTTGCCCAACTGGC‑GCTATGTCGACACCCAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAAGCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCCCCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCa          <  2:448189/248‑1 (MQ=255)                                                                                                                                                                                                                               gCGAAATCTTCCGCACCTTAAAAAAGGCCAATTAATTACAAACGGGCTTGGCGGAGACTCTGCCCAACTGGC‑GCTATGGCGACACAAAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTAcc  <  2:511534/251‑1 (MQ=255)                                                                                                                                                                                                                                            cACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGAc                                                                                             <  2:152347/147‑1 (MQ=255)                                                                                                                                                                                                                                            cACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGAc                                                                                             >  1:152347/1‑147 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     AAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAGAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGC‑GCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACC  >  NC_000913/1836152‑1836624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑