Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,799,792	C→T	intergenic (‑43/+10)	rplT ← / ← rpmI	50S ribosomal subunit protein L20/50S ribosomal subunit protein L35

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,799,792	0	C	T	100.0%	170.2 / NA	49	intergenic (‑43/+10)	rplT/rpmI	50S ribosomal subunit protein L20/50S ribosomal subunit protein L35
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/28);  total (21/28)

CGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACG  >  NC_000913/1799561‑1800033                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  cgcAAGCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTAc                                                                                                                                                                                                                                <  2:306829/251‑1 (MQ=255)  gcAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACg                                                                                                                                                                                                                               >  2:87071/1‑251 (MQ=255)  gcAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACg                                                                                                                                                                                                                               <  2:43720/251‑1 (MQ=255)        cacaGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACggcaggc                                                                                                                                                                                                                         >  1:406468/1‑251 (MQ=255)           aGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCCAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCAcg                                                                                                                                                                                                                      >  1:81769/1‑251 (MQ=255)                 cGGAACTGACGCTTCCGGTGACGACGGTCACGGTAAGCATACTGACCGGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTAAGCGAGCCATATGTGCTCTCCCGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGAGAttc                                                                                                                                                                                                                >  2:480171/1‑250 (MQ=255)                      cTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAgg                                                                                                                                                                                                           >  2:320456/1‑251 (MQ=255)                       tGACGCTTACGTTGACGACAGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGTAGGCAACGCGGTATACGCGACAACGAACACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TTACGTGCACGTGCAATAACACCACGTTTTACGAGAGCCATATGAGCTCTCCTGGATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCCGGCACGCGATTACCAgac                                                                                                                                                                                                          >  2:294091/1‑249 (MQ=255)                          cGCTTACGTGGACGTCGGTCACGTTAAGCAATCGGACCAGCTTTGATATCAGCCTGGAAGGCAACGCGGTATATGCGAGAACGCGCACCGTAGTAGCCTGTAGCTTTATTCAAAATTTTCTTG‑TGACGTGCACGTGCAATATCTCCACGTTTTACGCGATCCATATGTGCTCTCCTGTAGCTATATTCTAATTAAAAAGCTAAAAATGTTAACGGCTTATGCGTACGGCGGTCTCGAGATTACCAGGccta                                                                                                                                                                                                       <  2:540269/251‑3 (MQ=255)                                      cgacgGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTgg                                                                                                                                                                                           <  2:378001/251‑1 (MQ=255)                                                ggTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACTCCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCtt                                                                                                                                                                                                                                        <  2:128174/196‑1 (MQ=255)                                                ggTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCtt                                                                                                                                                                                                                                        >  1:128174/1‑196 (MQ=255)                                                       aTACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCGTTGGAAACCATGGCTTTCGGa                                                                                                                                                                          <  1:405721/251‑1 (MQ=255)                                                                     tGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGacgttt                                                                                                                                                            >  2:250719/1‑251 (MQ=255)                                                                     tGATAACAGCCGGGAAGGAAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACACGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGacgtt                                                                                                                                                             <  2:300301/250‑1 (MQ=255)                                                                      gATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGacgttt                                                                                                                                                            <  1:516009/250‑1 (MQ=255)                                                                       aTAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATTCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGacgtttac                                                                                                                                                          <  1:285992/251‑1 (MQ=255)                                                                           cAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGacgtttacgttt                                                                                                                                                      <  1:112530/251‑1 (MQ=255)                                                                                        aaCGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTg                                                                                                                                         >  1:164540/1‑251 (MQ=255)                                                                                                ataCGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGaa                                                                                                                                  <  1:134550/250‑1 (MQ=255)                                                                                                ataCGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAAt                                                                                                                                 >  2:216226/1‑251 (MQ=255)                                                                                                          aCGCGCACCGTAGTAGCTTTTAGCTTGTTTCAAAATTTTTTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAgg                                                                                                                       <  1:92563/251‑1 (MQ=255)                                                                                                                     agtagCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGacgtttacgttt                                                                                                                                                      >  2:346306/1‑209 (MQ=255)                                                                                                                     agtagCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGacgtttacgttt                                                                                                                                                      <  1:346306/209‑1 (MQ=255)                                                                                                                        agcctggagtttGTGTCAAAATTTGCTTG‑GGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGTCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgcttgt                                                                                                          <  1:542831/240‑1 (MQ=255)                                                                                                                         gCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGGGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgcttgtgc                                                                                                        <  2:430884/251‑1 (MQ=255)                                                                                                                          ccTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgcttatgct                                                                                                       >  2:45348/1‑251 (MQ=255)                                                                                                                             ttAGCTTGTTGTAAAATTTTCTTGGTG‑CGGGCACGTGCATTAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTa                                                                                                     <  2:117691/250‑1 (MQ=255)                                                                                                                                    gTTTCAAAATTTTCTTG‑TGGCGGGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGGTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAaccac                                                                                              <  1:474495/250‑1 (MQ=255)                                                                                                                                       tCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACCCCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCtt                                                                                           <  2:373553/250‑1 (MQ=255)                                                                                                                                                                    aaTAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAgcagca                                                              <  1:320456/251‑1 (MQ=255)                                                                                                                                                                     aTAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCGTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCAc                                                             <  2:178200/251‑1 (MQ=255)                                                                                                                                                                          accacGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACg                                                                                                                                                                        >  1:161418/1‑139 (MQ=255)                                                                                                                                                                          accacGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGAGTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACg                                                                                                                                                                        <  2:161418/139‑1 (MQ=255)                                                                                                                                                                               gTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGt                                                   >  2:309777/1‑251 (MQ=255)                                                                                                                                                                                ttttACGCGAGCCATATGTGCTCTCCTGTCTCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGAGCCGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTc                                                  >  1:179870/1‑251 (MQ=255)                                                                                                                                                                                      gcgAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAAtt                                            >  2:209722/1‑251 (MQ=255)                                                                                                                                                                                                tgtgCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCtt                                                <  1:65534/237‑1 (MQ=255)                                                                                                                                                                                                tgtgCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCtt                                                >  2:65534/1‑237 (MQ=255)                                                                                                                                                                                                 gtgCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCAttt                                  <  1:209722/250‑1 (MQ=255)                                                                                                                                                                                                   gCTCTCCTGTATCTATATTCTCATTAAAAAGTTCAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGCTCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGCCGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGATTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTta                                >  2:279103/1‑250 (MQ=255)                                                                                                                                                                                                   gCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTGTTTTTGAAGCGCTTAGCAGCACCGCGTACTGTCTTAATTTTTGGCATTTta                                <  2:81769/250‑1 (MQ=255)                                                                                                                                                                                                      ctcCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTtaat                              <  2:27515/249‑1 (MQ=255)                                                                                                                                                                                                      ctcCTGTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTtaat                              <  1:87071/249‑1 (MQ=255)                                                                                                                                                                                                           gTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttttt                                                                                                                                           <  1:51347/135‑1 (MQ=255)                                                                                                                                                                                                           gTATCTATATTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttttt                                                                                                                                           >  2:51347/1‑135 (MQ=255)                                                                                                                                                                                                                   atTCTAATTAAAAAGTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCGTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCa               <  2:469234/251‑1 (MQ=255)                                                                                                                                                                                                                                 gcgtaaaaTGTTAACGGCTTATGCGTACGGCAGAAACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACg  >  1:429976/5‑250 (MQ=255)                                                                                                                                                                                                                                 gTTAAAAATGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCCTTGTTAATAAACg  >  1:119820/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  CGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATAACAGCCTGGAAGGCAACGCGGTATACGCGAGAACGCGCACCGTAGTAGCCTTTAGCTTGTTTCAAAATTTTCTTG‑TGACGTGCACGTGCAATAACACCACGTTTTACGCGAGCCATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACG  >  NC_000913/1799561‑1800033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑