Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,792,541	C→T	G64E (GGA→GAA)	nlpC ←	NlpC/P60 family lipoprotein NlpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,792,541	0	C	T	100.0%	122.8 / NA	36	G64E (GGA→GAA)	nlpC	NlpC/P60 family lipoprotein NlpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/12);  total (24/12)

TTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATCCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATT  >  NC_000913/1792294‑1792765                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 tttCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAAACATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCg                                                                                                                                                                                                                               >  1:448778/1‑251 (MQ=255)            aGACATTATCAAGTGCGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGAATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAATACCAGGTCACCTGGCAGCAACTCGTCTTTATCACTTTGCGTGCCAACAGAGGCTTGTTGTTTGGTTTCCCGGAGCACCTGCCAATCGGAACGATCGCGCATCGTCACAACCACACATTCCGAACactcacc                                                                                                                                                                                                                     >  1:449771/1‑244 (MQ=255)                  tATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGTCCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGGCTTTATCAATGTGCGTGCCAATAGAGGCGTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCTTCGTCACAACCACAAATTCCGAACAGTCCTCAccgcg                                                                                                                                                                                                               <  2:550808/250‑1 (MQ=255)                   aTCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTGCACTGGTCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACAccgcgcc                                                                                                                                                                                                             <  1:545028/251‑1 (MQ=255)                      aaGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTCAATACCTACATGCAAACCATTTTGTACGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCGATTTGCGCGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCTGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACACATTCCGAACAGTCCACAccccgccgcg                                                                                                                                                                                                          >  2:532327/1‑251 (MQ=255)                              aaCGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATCGAGGCTTGTTGTTTGGTTTTACGGGGCAGCTGCAAAACGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCCCGCCGCATCATGca                                                                                                                                                                                                   >  2:124360/1‑249 (MQ=255)                                cGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTCAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGccacc                                                                                                                                                                                                >  2:200094/1‑251 (MQ=255)                                       cTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACg                                                                                                                                                                                          >  1:101148/1‑250 (MQ=255)                                                    agagGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTGTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGtgccat                                                                                                                                                                            <  2:462379/251‑1 (MQ=255)                                                                   ttGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGAACCCCGTTTTGAAAAAGACCAGGTAACCTGGCAGCAACTCGTCTTTATCAATTTGAGTGCACATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCt                                                                                                                                                             >  1:346426/1‑251 (MQ=255)                                                                            ggTATCGTAAATCCCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGACACGATCGCGCATCGTCACAACCACCAATTCCGAACAATCCAAACCGCGCCGCGTCATGCCACCCTAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTc                                                                                                                                                     >  1:550049/1‑250 (MQ=255)                                                                                                gCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCCGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATc                                                                                                                                >  2:55341/1‑251 (MQ=255)                                                                                                      cATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGG‑CAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATc                                                                                                                           >  2:326393/1‑249 (MQ=255)                                                                                                                      cGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTg                                                                                                          >  2:465466/1‑251 (MQ=255)                                                                                                                          ttGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCCTGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTggcgg                                                                                                      >  2:165146/1‑251 (MQ=255)                                                                                                                                           aCCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCCCGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGGACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGACAGGCTGGCATTTGGCGGCGGTGCTTTAttgtggg                                                                                     >  2:166655/1‑250 (MQ=255)                                                                                                                                             cTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTAAAACCGGCAATAACGGGAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGGGGCt                                                                                    >  1:315398/1‑250 (MQ=255)                                                                                                                                               ggCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTa                                                                                   <  2:20890/249‑1 (MQ=255)                                                                                                                                                gcagcaACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAAAGGGAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTacac                                                                                >  1:256443/1‑251 (MQ=255)                                                                                                                                                          gTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCATCTGCAAATCGAAACGCTAGAGCATCGTCACAACCACAAATTCCGGACAGTCCACACCGCGACGAGTCATGCCCCCATAACCATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCACTAACGGTCATCGACTAAGAAAGTCTTGCATTTGGCGGCGGTGCTTTATGGTCGCTACACCCggaccga                                                                       >  1:80578/1‑245 (MQ=255)                                                                                                                                                              ttATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGc                                                                    <  2:448778/249‑1 (MQ=255)                                                                                                                                                              ttATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTGCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACTCCCGGCCAGAAGCa                                                                   <  1:55341/250‑1 (MQ=255)                                                                                                                                                               tATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAgg                                                                 >  1:467582/1‑250 (MQ=255)                                                                                                                                                                aTCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGt                                                                 <  1:200094/250‑1 (MQ=255)                                                                                                                                                                        gCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATc                                                        <  2:217037/251‑1 (MQ=255)                                                                                                                                                                             ccAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTCGCTGGTCGTTCAAACCGGCAATAACGGGAATCGAATCAGAAAGGCTGGCATTTGGCGGCGGTGCTTTATGGTGGACACACCCGGCCAGAAGCAGTGCTGGGATCAAAAt                                                   >  2:523753/1‑251 (MQ=255)                                                                                                                                                                              cAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGGGGTGGGATCAAAAt                                                   >  1:204366/1‑250 (MQ=255)                                                                                                                                                                                    agGCTTGTTGTTTGGTTTCACGGG‑CAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGa                                           <  1:326393/251‑1 (MQ=255)                                                                                                                                                                                        ttgttgTTTGGTTTCACGGGGCAGCTACAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGATCAAACCGGCAATAACGGGAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCGTTATGGTGGCTAACCCCGACCCGAAGCAGTGCTGTGGTCAAAATAAGGCAGAAAc                                        >  2:144445/1‑251 (MQ=255)                                                                                                                                                                                           ttgttTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGGTCAAAATAAGGAAGAAACGc                                      >  2:103979/1‑250 (MQ=255)                                                                                                                                                                                             ttttGGTTTCACGGTGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGGCGTGCAAACCGGCAATAACGGTAAGCGAATCAGAAAGTCTGGCATTGGGCGGCGGTGCTTGATGGTGGCTACGCTCGGCCAGAACCAGTTCTGTGATCATAATCAGGCAGGAACGCAt                                    <  2:77213/249‑1 (MQ=255)                                                                                                                                                                                              tttGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAAtca                                                                                                                                        >  2:176815/1‑147 (MQ=255)                                                                                                                                                                                              tttGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATaa                                                                                                                                        <  1:176815/149‑1 (MQ=255)                                                                                                                                                                                                 ggTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGAGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCg                                <  2:155193/250‑1 (MQ=255)                                                                                                                                                                                                                           aaCGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGc     >  1:441559/1‑251 (MQ=255)                                                                                                                                                                                                                               aTCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGATACCCCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTCTTCTTGCAtt  >  1:394887/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 TTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATCCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATT  >  NC_000913/1792294‑1792765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑