Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,748,841	C→T	R240K (AGA→AAA)	ydhT ←	uncharacterized protein YdhT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,748,841	0	C	T	97.8%	145.3 / NA	45	R240K (AGA→AAA)	ydhT	uncharacterized protein YdhT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/20);  minor base G (0/1);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAAT  >  NC_000913/1748596‑1749082                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  cGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGGCACCGGCTCGAATATTTTTTTCACCTTCAATGGCGCAGGCGAAGCGAACTTTTTCg                                                                                                                                                                                                                                              >  2:397765/1‑251 (MQ=255) cGTTCGGCGCGTTGCACTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCg                                                                                                                                                                                                                                              <  1:263802/251‑1 (MQ=255)  gTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGc                                                                                                                                                                                                                                             >  1:211945/1‑251 (MQ=255)      ggCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGGGCAGAAATTGGTGAGGCGCTGGATCGGGGGGTAGTAAAGACTGCATCCCGTGCTCGTCGGCGTCTTTAGTTTGATTAAAGGTAAAGCTATCAGCAATTAACCAAATGATGCTGTTCATTAAGAAGATAGAGGACTCCGGCTCGAATATTGTTTTCACCGTCAATGTCGACGGGGAAGCGTACTTTTTCGCCgac                                                                                                                                                                                                                                         >  1:264119/1‑249 (MQ=255)             tGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACGTGCGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAATCTTATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTGTTTCGCCGTCATGata                                                                                                                                                                                                                                   <  2:265283/250‑1 (MQ=255)                               aCCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTtcct                                                                                                                                                                                                                >  1:21213/1‑251 (MQ=255)                                                        ggCATGTGCAGAAATTGGAGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCAGGCCCGAGAATAATTGAGTCg                                                                                                                                                                                        >  1:374182/1‑250 (MQ=255)                                                                                              tCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAACGCTCTCCGCACTCACCAAACTGATGCTGTTCATTAAGACGATAGCGGAATGCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGACACGCACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCCCTACCGTTGCATTTTCACCGCTg                                                                                                                                                 >  2:321560/1‑251 (MQ=255)                                                                                              tCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTg                                                                                                                                                 >  1:455790/1‑251 (MQ=255)                                                                                                 tAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGATGGc                                                                                                                                               >  1:486631/1‑250 (MQ=255)                                                                                                      ccTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgat                                                                                                                                         <  1:435321/251‑1 (MQ=255)                                                                                                         gCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACCCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCTCCGCTGGCAATGAtgt                                                                                                                                       <  1:184268/250‑1 (MQ=255)                                                                                                                  gCTCGTCGGCGTTTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTc                                                                                                                             <  1:332026/251‑1 (MQ=255)                                                                                                                           cGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGtt                                                                                                                    >  1:327206/1‑251 (MQ=255)                                                                                                                                   gTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGCCGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTc                                                                                                            >  2:526032/1‑251 (MQ=255)                                                                                                                                   gTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTc                                                                                                            >  1:129764/1‑251 (MQ=255)                                                                                                                                      tGATTAAAGGTAAAGCGATCAGCACGCAACAAACTGATTCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCTCCTTCAATGGCGACGGCGAAGCTAACGTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGGCCACGCTCGTGCTCTCTTCCGTTTAATTTTTACCGCTGGCAATTATGGGTATAGTGTCGCCGCTGTTGGTTATTCTg                                                                                                          <  2:498993/250‑1 (MQ=255)                                                                                                                                        aTTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCg                                                                                                       >  1:344946/1‑251 (MQ=255)                                                                                                                                              ggTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACCTTGTCGCCGCTGTTGGCAATTCTGGCGTTTgcg                                                                                                 >  1:229589/1‑251 (MQ=255)                                                                                                                                                  aaGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCaa                                                                                             <  2:455790/251‑1 (MQ=255)                                                                                                                                                   aGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTc                                                                                                                                                                         >  1:164399/1‑174 (MQ=255)                                                                                                                                                   aGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTc                                                                                                                                                                         <  2:164399/174‑1 (MQ=255)                                                                                                                                                     cTATCAGCACTCAACACACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCACGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCACAGc                                                                                          >  1:451610/1‑251 (MQ=255)                                                                                                                                                       aTCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTg                                                                                        <  2:211945/251‑1 (MQ=255)                                                                                                                                                         cAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACGCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCATTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGGCAAAGCTGGc                                                                                      <  2:208876/251‑1 (MQ=255)                                                                                                                                                           gCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGa                                                                                    <  1:161162/251‑1 (MQ=255)                                                                                                                                                                  acaaACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCAc                                                                             <  2:255763/251‑1 (MQ=255)                                                                                                                                                                  acaaACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCAc                                                                             >  1:289536/1‑251 (MQ=255)                                                                                                                                                                           tGCTGTTCATTAAGACGATTTCGTACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGCCGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCATGGCCCGAGAATATTTGAGTCTACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCACTTCTGGCGTTTGCGCCACAGCTGGCGATTTGCAACAGGTCAcc                                                                    >  2:66250/1‑251 (MQ=255)                                                                                                                                                                                ttCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGAtt                                                                                  >  2:22010/1‑232 (MQ=255)                                                                                                                                                                                ttCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGAtt                                                                                  <  1:22010/232‑1 (MQ=255)                                                                                                                                                                                  cATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCTAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGGGCGTACTACCGTTGAATTTTCACCGTGGGCAATGATGTGTACATTTTCTCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTGGCACCAGGTCACCATTACt                                                              <  1:182825/250‑1 (MQ=255)                                                                                                                                                                                  cATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTg                                                             <  2:424937/251‑1 (MQ=255)                                                                                                                                                                                   atgaaGACGATAGCGGACTCCGGCTCGAATATTGTTTTCTCCTTCAATGGCGACGGCGAAGCGAGCTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGGGATGATGTGTACATTGGCGCCGCTGTTGGCAAGTCTGGCGGTTGCGCCAAAGCTGGCGATTTGCACCAGGTGACCATGACTgg                                                            <  2:366064/248‑1 (MQ=255)                                                                                                                                                                                             tAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTACTGGCCCGAGAATAATTGAGTCGACCACGCACGTACTCACTACCGTTGAATTTTCACCGCTGGCACTGATGTGTCCATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGac                                                   >  1:323446/1‑250 (MQ=255)                                                                                                                                                                                              aGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAAGTGAGTCGACCACGCCCGTGCTCACTACCGGTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCGGGCGATTTGCACCAGGTCACCATTACTTGCGACATGacac                                                 <  2:377269/251‑1 (MQ=255)                                                                                                                                                                                               gCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACAcc                                                <  1:438909/251‑1 (MQ=255)                                                                                                                                                                                                cGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACCTTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAACGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGaccacg                                               >  1:182148/1‑247 (MQ=255)                                                                                                                                                                                                          cTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCGATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAAt                                     >  1:283350/1‑251 (MQ=255)                                                                                                                                                                                                                         cACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTAc                       <  1:397765/250‑1 (MQ=255)                                                                                                                                                                                                                         cACCTTCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACt                      <  1:55608/251‑1 (MQ=255)                                                                                                                                                                                                                           ccttcagtgggggcggacaagcgaaCTTTTTCCCCGTCCTGATATGCCAGTGCGGCGCTTACTCCTGGCCCGAGAATACTTGCGTCGACCACACCCGTGCTCACTAGCGTTGAATTTTCACCGCTGGCACTGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCCCCCAAAGTAAAAACCCGTaaccg                    >  2:247207/19‑247 (MQ=255)                                                                                                                                                                                                                             ttCAATGGCGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGc                   >  1:259455/1‑250 (MQ=255)                                                                                                                                                                                                                                     cGACGGCGAAGCGAACTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTTGCAAGTCTGGCGTTTGCGCCAAAGCTGGTGATGTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTa          <  1:514040/251‑1 (MQ=255)                                                                                                                                                                                                                                             aagcgaaCTTTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCCCGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACAAGGTCACCATTACTGGCGACATGACACAGTTCCCCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAAt  >  2:457340/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAAT  >  NC_000913/1748596‑1749082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑