Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,712,919	C→T	Q51* (CAA→TAA)	dtpA →	dipeptide/tripeptide:H(+) symporter DtpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,712,919	0	C	T	100.0%	103.5 / NA	29	Q51* (CAA→TAA)	dtpA	dipeptide/tripeptide:H(+) symporter DtpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/12);  total (17/12)

ATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAACAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGG‑CTATTGCGGTCGGTAACGGCCTGT  >  NC_000913/1712689‑1713153                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            ataACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAg                                                                                                                                                                                                                           <  1:68188/249‑1 (MQ=255)                         ataCATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTcc                                                                                                                                                                                                 <  1:473197/251‑1 (MQ=255)                                    gagaAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGAAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTAAATCACCCTTTTCTCTTCCTTTAGTGCCCt                                                                                                                                                                                      >  1:470706/1‑251 (MQ=255)                                                                                      cACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTgg                                                                                                                                    >  2:499469/1‑251 (MQ=255)                                                                                      cACTGCAAACCAAAAACCAACTGAAAGCGTCAGGTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCTCCGTTTTCTCTTCCTTTAGTGCTCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTgg                                                                                                                                    <  1:552571/251‑1 (MQ=255)                                                                                                caaaaaccaaCTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGCCAAGGTACTGGGTGCTTAAcg                                                                                                                          >  1:299820/1‑251 (MQ=255)                                                                                                             aaaGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTc                                                                                                             >  2:453719/1‑251 (MQ=255)                                                                                                              aaGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCg                                                                                                            <  1:459001/251‑1 (MQ=255)                                                                                                                 cGTCAGTTTGAACGCTTACCAACAACCGAAGGCGTTAGATCTCATCTTCTCGATTGAGTTATGGGCACGATTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACATGGTTAAATGACTGGGTATGTCTGAAGCGGATTCAATAACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTTTGGTCGCTATCGGCGGCTGGTTAGGTGAAAAAGGAATGGGGACTTAACGCGTAATTTTGCTCgggg                                                                                                         >  2:274532/1‑249 (MQ=255)                                                                                                                                           cGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTg                                                                                              >  2:186971/1‑236 (MQ=255)                                                                                                                                           cGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTg                                                                                              <  1:186971/236‑1 (MQ=255)                                                                                                                                                   ttCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGCATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGc                                                                       >  1:34607/1‑251 (MQ=255)                                                                                                                                                               ttctCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGGACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCtggtctggtc                                                            >  2:26127/1‑250 (MQ=255)                                                                                                                                                                    gATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCacgacg                                                      >  1:46880/1‑251 (MQ=255)                                                                                                                                                                        gAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCgg                                                  <  1:350361/251‑1 (MQ=255)                                                                                                                                                                               gggAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGGATCGtt                                           >  1:403177/1‑251 (MQ=255)                                                                                                                                                                                       tttGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTCTGACTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTTGGTGACCAGGTACTGGGTACTAAACGAGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTTTGCCCTGGATGCCTGGTCTGGTCACGACGCCGGGCTCGTTTATATggg                                   >  2:289994/1‑251 (MQ=255)                                                                                                                                                                                              attaCGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTc                                                                                                             <  2:123678/170‑1 (MQ=255)                                                                                                                                                                                              attaCGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTc                                                                                                             >  1:123678/1‑170 (MQ=255)                                                                                                                                                                                                      cTACAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCCATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGAGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGG‑CTATTGc                   >  1:52011/1‑251 (MQ=255)                                                                                                                                                                                                         cAAGGAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGGCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCTGTATCGTTTATATGGGTATGGCGT‑CTATTGcgg                 <  1:122438/250‑1 (MQ=255)                                                                                                                                                                                                             gAATTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGTCTCGTTTATATGGGTATGGCGG‑CTATTGcggtaggg            >  1:475633/1‑247 (MQ=255)                                                                                                                                                                                                               aTTATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGG‑CTATTGCGGTCGGTaa          <  1:499469/251‑1 (MQ=255)                                                                                                                                                                                                                 tATGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGG‑CTATTGCGGTCGGTAAc         <  1:453719/250‑1 (MQ=255)                                                                                                                                                                                                                  aTGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGG‑CTATTGCGGTCGGTAACgg       <  1:207322/251‑1 (MQ=255)                                                                                                                                                                                                                  aTGGCTGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGATTATATGGGTGTGGCGG‑CTATTGCGGTCGGTAACgg       >  1:290263/1‑251 (MQ=255)                                                                                                                                                                                                                       tGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCacgacg                                                      >  1:71509/1‑200 (MQ=255)                                                                                                                                                                                                                       tGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCacgacg                                                      <  2:71509/200‑1 (MQ=255)                                                                                                                                                                                                                       tGTTTACCTGGTTAAATAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGGTTATTGCGG‑CGGTAACGGGCTGt  >  1:9167/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            ATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGTTTACCTGGTTAAACAACTGGGTATGTCTGAAGCGGATTCAATCACCCTTTTCTCTTCCTTTAGTGCCCTGGTTTATGGTCTGGTCGCTATCGGCGGCTGGTTAGGTGACAAGGTACTGGGTACTAAACGCGTAATTATGCTCGGCGCTATTGTGCTGGCGATTGGTTATGCTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGG‑CTATTGCGGTCGGTAACGGCCTGT  >  NC_000913/1712689‑1713153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑