Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,712,661	A→T	intergenic (+503/‑108)	nth → / → dtpA	endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,712,661	0	A	T	100.0%	44.2 / NA	16	intergenic (+503/‑108)	nth/dtpA	endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/6);  total (10/6)

TGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGAACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGT  >  NC_000913/1712455‑1712905                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      gggACTATCTCACTAGAAAAACCGCTAAATAGCAGAACATATCGCCGTTAAGCCACCACCCCCCCCCCAGTGCAGTGAAAAAATCGGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTAt                                                                                                                                                                                                           <  1:343000/250‑1 (MQ=255)          tcACTAGCAAAACCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTATCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCACGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGAAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTtat                                                                                                                                                                                                  >  2:552571/1‑251 (MQ=255)                   aaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCATCTCCGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAAAGCTGGATACCATTTCCCGGAATGGTTGAATTCACCGCCTCAGTTATATGTAAAAGATTATTACAACGGACTTGTCTGAAAGTGCAAGATAGTGTACATTCCCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTCTGTTTTTTATACATAAcgc                                                                                                                                                                                         >  1:493728/1‑251 (MQ=255)                                                                      gtgcagtgAAAAAATCTGCCGTTACGTTTTTTGAAACATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GAAAAAGTGTcc                                                                                                                                     >  2:207322/1‑251 (MQ=255)                                                                        gcagtgAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGAAAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAAATAGTGTACATTACCTGCCGTTTCCCCTCCCACTCTAACACTTGCGCGGATGTTTATTATACATAACGCGAGAAAGCACCCCCGTTCATAAGGGATGTAAAAAAA‑AGAGTAAAAGTGTCCAc                                                                                                                                   >  2:122438/1‑251 (MQ=255)                                                                            ggAAAAAATCTGCTGTTTGGTTTTTGGAAAATTTTACCGCGGGAGAATATTCCCAAGACTGTTTGACTCCCCCGCCTCAGTTATATGTATCAGATGATTACAAAGGGCTTGCCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATATCAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCCCCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCa                                                                                                                               <  1:32932/250‑1 (MQ=255)                                                                                               gTTTTTTGAAAACTTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTCTATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGaa                                                                                                             >  2:68188/1‑250 (MQ=255)                                                                                                             ttAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAAc                                                                                                                                                                                           >  1:178249/1‑159 (MQ=255)                                                                                                                        aaacattcccgtgatgggTTGAATTCACCGCCTCAGTTATAGGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAGCGCTTTCAaaca                                                                                   <  2:328800/235‑1 (MQ=255)                                                                                                                           cATTTCCCGTAAGGTTTGAATCCTCCGCCTCAGTTATATGTAACATATTATTACAATGGACTTGTCTGAAAGTGCAAGATATTGTACATTCCCTGCCGTTTCCCCTCCCTCTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAAcc                                                                                <  1:378089/251‑1 (MQ=255)                                                                                                                                cccGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAgg                                                                           >  1:356844/1‑251 (MQ=255)                                                                                                                                   gaattggtaaatttCCCCTCCTCAGTTATAGGTAAAAGATTATTACAAAGGACTTGTCGGAAAGTGCAAGATAGTGTACATTGCCTTCCGTGTCCCCTCCCACTATAACAATGGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTGTGAACGCTTGCAAACAACCCAAGGCg                                                                         <  2:235680/238‑1 (MQ=255)                                                                                                                                           tGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGAGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTAtctc                                                                >  2:473197/1‑251 (MQ=255)                                                                                                                                                     gCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTCCTCGa                                                      <  2:227013/251‑1 (MQ=255)                                                                                                                                                                                      aCTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCCCTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAACCCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGACGGCGTTCTATCTCATCTTCTCGATTGAGATATGGGAACGTTTTGGTTATTACgtcc                     >  2:1986/1‑248 (MQ=255)                                                                                                                                                                                                         aTAGTGTACATTACCTGCCGTTTCCCCTCCCACTATCACACTTGCGCGTATGTTTTTTAAACATAACGCGAGCACGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGt  >  2:213561/1‑251 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      TGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGAACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAG‑GTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACGTTTTGGTTATTACGGCCTACAAGGAATTATGGCTGT  >  NC_000913/1712455‑1712905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑