Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,460	G→A	Q369* (CAG→TAG)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,460	0	G	A	100.0%	126.6 / NA	42	Q369* (CAG→TAG)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/18);  total (24/18)

CAGCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGC  >  NC_000913/1667214‑1667705                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cAGCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACt                                                                                                                                                                                                                                                    >  1:3392/1‑250 (MQ=255)       cACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCa                                                                                                                                                                                                                                             <  2:162575/251‑1 (MQ=255)                                        aCCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGATATACATCGCGTCTTTTACCAGTGCAGCGCATGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATg                                                                                                                                                                                                            >  1:309997/1‑251 (MQ=255)                                             gAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCgg                                                                                                                                                                                                       <  1:71965/251‑1 (MQ=255)                                                 ataatTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGa                                                                                                                                                                                                     >  2:33723/1‑249 (MQ=255)                                                       aaCCTTGAAAGTCTAAGTGATGCATTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGGTAAAAAAGGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGata                                                                                                                                                                                             <  1:289012/251‑1 (MQ=255)                                                         ccgtcaaaGTCTAAGTTATGAGTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTACCCCTGCAACCGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATCAGCAGGAAGGGCCTGCTGACGGATGCTGTCGGAGATGACCGGGAAGAGTATATc                                                                                                                                                                                           <  2:467169/246‑1 (MQ=255)                                                             gAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGcc                                                                                                                                                                                       <  1:33723/251‑1 (MQ=255)                                                                aGTCTAAGTTATGCTTTCCTGGCCCAAATTGCGATAGCGCAAATTCTGGTAGAACAGTTAAAAAATGTTACACCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGACTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAAAGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCt                                                                                                                                                                                    >  2:505227/1‑251 (MQ=255)                                                                    tAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACTAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGTTTAGAACACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTCCCGCTTTAc                                                                                                                                                                                >  1:449888/1‑251 (MQ=255)                                                                      aGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTCGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTCCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGCTGACCGGGAAGAGGATCTCTGCCGCtttcatt                                                                                                                                                                              >  2:210230/1‑247 (MQ=255)                                                                                 ccTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGcc                                                                                                                                                                   >  2:31375/1‑251 (MQ=255)                                                                                      cccAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATc                                                                                                                                                               >  2:181263/1‑250 (MQ=255)                                                                                           aTTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATAGCGTCTTTTACCAGTGCAGCGACTGCCATCGTGCCCTGGTTAGAAACCTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtt                                                                                                                                                         >  2:162645/1‑251 (MQ=255)                                                                                              gagaTAACGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACCAAGAACCGTTATACATCGCGTCTTTTACCAGTCCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTCCTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCACTCAGTAttttt                                                                                                                                                      >  2:369096/1‑251 (MQ=255)                                                                                                                  aGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGCACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                                                                                  >  2:339184/1‑251 (MQ=255)                                                                                                                       aGTTAAAAAATGTTAACACTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGAATTTTTTGTGGGTTAAATAAATTCACCatg                                                                                                                              >  2:291174/1‑250 (MQ=255)                                                                                                                                 tGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                                                                                   >  2:283602/1‑251 (MQ=255)                                                                                                                                          tgccaCAGACGAATCGACAAAGAACCGTTCTACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCATGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGTTGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTAAAATAAATTCACCATGATGGCACGAAttccactga                                                                                                           >  2:74860/5‑242 (MQ=255)                                                                                                                                                         aacaaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAcccc                                                                                           >  1:194326/1‑251 (MQ=255)                                                                                                                                                            aaaGAACCGTTATCCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGc                                                                                                                                                                    >  2:82445/1‑175 (MQ=255)                                                                                                                                                            aaaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGc                                                                                                                                                                    <  1:82445/175‑1 (MQ=255)                                                                                                                                                                        taCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCttt                                                                            >  1:265441/1‑251 (MQ=255)                                                                                                                                                                                         cAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgccgc                                                           <  2:194326/251‑1 (MQ=255)                                                                                                                                                                                          aGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGGACTCTCAACGCTGATGTGCGAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGACGCTTTACGTAACGGTTAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTATTTTGCCAGTAGATcgccgcc                                                          <  2:449888/251‑2 (MQ=255)                                                                                                                                                                                                 cgcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAACTTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCCGTAGATCGCCGCGCAATgcc                                                   >  1:348708/1‑251 (MQ=255)                                                                                                                                                                                                     tGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTg                                                                                                                                                                                         <  1:367894/113‑1 (MQ=255)                                                                                                                                                                                                     tGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTg                                                                                                                                                                                         >  2:367894/1‑113 (MQ=255)                                                                                                                                                                                                      gCCATCGTGCCCTGGTTCGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCCATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGAGCCCACCCAGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTg                                              >  2:490552/1‑251 (MQ=255)                                                                                                                                                                                                         aTCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGaca                                           <  1:283602/251‑1 (MQ=255)                                                                                                                                                                                                           cGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCACGGTAATGATGTCTTTTGCACGTAGATCGCCGCGCAATGCCGCCTGacac                                          >  1:467798/1‑250 (MQ=255)                                                                                                                                                                                                              gCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATg                                      <  1:339184/251‑1 (MQ=255)                                                                                                                                                                                                               cccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCTCCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGa                                     <  2:89357/251‑1 (MQ=255)                                                                                                                                                                                                                     ttAGAAAACTGAGTCCTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTATCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGAGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCCATGCCGCCTGACACAATGAGTCCa                                >  2:511072/1‑250 (MQ=255)                                                                                                                                                                                                                                aGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGtt                    <  1:31375/251‑1 (MQ=255)                                                                                                                                                                                                                                aGTACTCTCAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGtt                    >  1:209285/1‑251 (MQ=255)                                                                                                                                                                                                                                 gagctctCAACGCTGATGTGCTAACTATAGGCAGGAAGGGCCTGCTGACGAATGATGTCTGAGATGACGGGGAAGAGGATATCTGCCGCTTTGCTTAACGTGGAGCCAAACAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCGAAAATGCGCCCGACTTGCGCGCCCCCCCCGGGAATGATGTCTATTGCCGGTAGTGCGCCGGGCAGTGCCACCTGACACAATGAGTCCacggttgacggttg                   <  2:309997/248‑1 (MQ=255)                                                                                                                                                                                                                                   gctctCAACGATGATGTGCTAACTATACGCAAGAATGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGGTAAATAAATTCGCCATGATGGCAAGAATGCGCCCGACATTCGCGTCCACCCCGGTAATGATGGCTTTTGCCAGGAGATCGCCGCGCAATGCCGCTTGACGCAATGATTCCGCGGTTAAGGGTTGTc                 <  2:68047/250‑1 (MQ=255)                                                                                                                                                                                                                                       tcAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATg             >  1:376170/1‑251 (MQ=255)                                                                                                                                                                                                                                        cAACGCTGATGTGCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATGTGTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCGCCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCTCCTGACACAATGAGTCCACGGTTAACGGTTGTCCAt              <  1:505227/249‑1 (MQ=255)                                                                                                                                                                                                                                            gCTGATGTGCTAACTATACGGAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGATCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACa        <  1:335220/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gtgCTAACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCTGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTGGTCCATGTAACATCGAGc  <  1:210230/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CAGCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGTAACATCGAGC  >  NC_000913/1667214‑1667705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑