Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	292,019	C→T	L174L (CTA→TTA)	yagJ →	protein YagJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	292,019	0	C	T	100.0%	171.1 / NA	50	L174L (CTA→TTA)	yagJ	protein YagJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/30);  total (20/30)

CATTTGTTGTGTGACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAAC  >  NC_000913/291773‑292256                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              cATTTGTTGTGTTACGTAAGCGGGAACTATCCGGCTCTGTTGAACTTTTGGGAAACCAAGGTTTTTGTGATCGAATCCAAACGGGAAGGTTTTGCTTTCTGGTATCGTAATCCACAGAACACAGGTCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCTTGTTCTTTGCCGAACAGGATAGCAAAATGGTTGTGGACCTGGTAGATCCCCATAGCTTAc                                                                                                                                                                                                                                            >  2:282743/1‑250 (MQ=255)         gtgtgACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGGAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTg                                                                                                                                                                                                                                   <  1:89325/251‑1 (MQ=255)         gtgtgACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTg                                                                                                                                                                                                                                   >  2:387179/1‑251 (MQ=255)             gACGAAAGAGGGAACTATCCGGCTCTGTTGAACCATTGGGAGACCCAGGGTTGTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGACGTCACTGGGACTCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCAGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCAGATGc                                                                                                                                                                                                                               >  2:25523/1‑251 (MQ=255)               cGAAAGCGGGAACTATACGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCtt                                                                                                                                                                                                                             <  2:70121/251‑1 (MQ=255)                gAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCACGGTTTTTGAGATCGAAACCAAACGTGCAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCttt                                                                                                                                                                                                                            >  2:59909/1‑251 (MQ=255)                   aGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGcc                                                                                                                                                                                                                         <  1:158067/251‑1 (MQ=255)                       ggAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATg                                                                                                                                                                                                                                <  2:239575/240‑1 (MQ=255)                       ggAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATg                                                                                                                                                                                                                                >  1:239575/1‑240 (MQ=255)                         aaCTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACt                                                                                                                                                                                                                   <  1:8883/251‑1 (MQ=255)                          ccTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGGTTTTGAGATCGAAACAACACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTCTGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGGCCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTg                                                                                                                                                                                                                  >  2:429490/2‑251 (MQ=255)                                         tGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCt                                                                                                                                                                                                    <  1:496886/250‑1 (MQ=255)                                                   ggAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCaaa                                                                                                                                                                                                                     >  1:135402/1‑223 (MQ=255)                                                   ggAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCaaa                                                                                                                                                                                                                     <  2:135402/223‑1 (MQ=255)                                                           aGGTTGTTGAGATCGAAACCAAACGTGAAGGATTTGCTGTCTGGTATCGGAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTGTGCCCAAACTGGAAGGACGTGCGCTATATGCCGAACATCATTCt                                                                                                                                                                                 <  2:263413/251‑1 (MQ=255)                                                              tttttGCGATCGACACCAAACGAGAAGGATTTGCTTTCTGGTATCGTAATCCCCAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTCGATCCACATAGCTTAAATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGAt                                                                                                                                                                              >  2:99673/1‑251 (MQ=255)                                                                   gagaTCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTGTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTa                                                                                                                                                                         <  2:508532/251‑1 (MQ=255)                                                                     gaTCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACa                                                                                                                                                                       <  2:277218/251‑1 (MQ=255)                                                                       acGAAACCAAACGTGAAGGATTTGCTTGCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACAGAGCTTAGATCTGGCTGATGCTTTGCCCAAACTGGAAGGACGTGCGCTATATGCCGAACATCATTCTGGTGCTTACAgg                                                                                                                                                                     <  2:235857/250‑1 (MQ=255)                                                                             ccAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGAACACAGGGCCGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTACCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTAAAGGcgaatc                                                                                                                                                               >  2:523044/1‑251 (MQ=255)                                                                                  cGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCgaatcgcatc                                                                                                                                                          >  1:264058/1‑251 (MQ=255)                                                                                             tGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGaa                                                                                                                                                <  1:261128/250‑1 (MQ=255)                                                                                                        aTCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGcc                                                                                                                                                                                             >  2:318521/1‑194 (MQ=255)                                                                                                        aTCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGcc                                                                                                                                                                                             <  1:318521/194‑1 (MQ=255)                                                                                                        aTCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCTCCGAAGTAAAGGGTaa                                                                                                                                     <  1:186834/250‑1 (MQ=255)                                                                                                           gTAATCCCCAGTACACCAGGCCGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCATCCCGATTTCATGTTCTTTGCCGAACAGGATGGAAAAATGGTTGTGGAACTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGCATCGCATCTGTCGCCGAAGAAAAGGGTaactt                                                                                                                                  >  2:289525/1‑247 (MQ=255)                                                                                                           gTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAAtt                                                                                                                                  >  2:381783/1‑250 (MQ=255)                                                                                                                                aGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACgg                                                                                                            >  2:142160/1‑251 (MQ=255)                                                                                                                                    gtcACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAgg                                                                                                        >  2:2383/1‑251 (MQ=255)                                                                                                                                       aCTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCTCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGAtg                                                                                                     <  1:33587/251‑1 (MQ=255)                                                                                                                                          gggAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGCAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGc                                                                                                  >  1:349611/1‑251 (MQ=255)                                                                                                                                                     aTGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACAGCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGtt                                                                                       <  1:440782/251‑1 (MQ=255)                                                                                                                                                      tGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTg                                                                                      >  1:533206/1‑251 (MQ=255)                                                                                                                                                       gTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTCCGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGc                                                                                     >  1:162807/1‑251 (MQ=255)                                                                                                                                                          gAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATGTGAAACGGCAGGATGTGCAGGATGCTGTTGCTAc                                                                                  <  1:207830/251‑1 (MQ=255)                                                                                                                                                              cTGAACAGTCCAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGCACAGTATGGCAACATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATCTGCCGAACATCATTCTGATGCTTACAGGCGCATCGAATCTGTCGCCGAAGTCAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCt                                                                              >  2:81498/1‑251 (MQ=255)                                                                                                                                                                gAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTg                                                                             <  1:349544/250‑1 (MQ=255)                                                                                                                                                                              tGTTCTTCCCGATTTCCTGTTCTTTGGCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCTCATAGCTTACATCTGGCTGATGCTTTACCCAAACTGGAAGGACTGTCGCTATATTCCGAACATCATGCTGATGCTTACAGGCGAATCGAATCTGGCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAGCGGCAGGATGTGCAGGATGCGGTTGCTACCGCGGAAAATGCAGAAACg                                                               <  2:41442/250‑1 (MQ=255)                                                                                                                                                                                   gTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTAttt                                                         <  1:145562/251‑1 (MQ=255)                                                                                                                                                                                         aTTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGc                                                   <  2:527590/251‑1 (MQ=255)                                                                                                                                                                                             ccTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGAc                                               <  1:2383/251‑1 (MQ=255)                                                                                                                                                                                             ccTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCGTGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTGAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGAc                                               <  2:487633/251‑1 (MQ=255)                                                                                                                                                                                                          gAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGaaaa                                                                         <  1:217547/212‑1 (MQ=255)                                                                                                                                                                                                                   ggCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAAt                          <  1:11749/250‑1 (MQ=255)                                                                                                                                                                                                                        aaTGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAg                    <  2:10245/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGc            <  1:142160/250‑1 (MQ=255)                                                                                                                                                                                                                                       ggTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAAc       <  1:381783/249‑1 (MQ=255)                                                                                                                                                                                                                                       ggTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACg      >  1:212344/1‑250 (MQ=255)                                                                                                                                                                                                                                          aGATCCACATAGCTTACATCTGGTTGATGCTTTGCCCTAACTGGAAGGACTTGCGCTATATGCCGAACAACATTCTGATGCTTACAGGCGAATCGAATCTGGCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTAACAGTAATCTATAGAAAATTGCGTAACGaaa   <  2:378069/250‑1 (MQ=255)                                                                                                                                                                                                                                          aGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCTTACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAAc  <  1:35270/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              CATTTGTTGTGTGACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAAC  >  NC_000913/291773‑292256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑