Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,247	C→T	I11I (ATC→ATT)	ydfZ →	putative selenoprotein YdfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,247	0	C	T	100.0%	105.9 / NA	32	I11I (ATC→ATT)	ydfZ	putative selenoprotein YdfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/15);  total (17/15)

GCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAT  >  NC_000913/1629016‑1629493                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        gCGACTGGTTTAGCGAAGAGTAATTTATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTa                                                                                                                                                                                                                                      <  2:396873/251‑1 (MQ=255)        gTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATtacca                                                                                                                                                                                                                                                     >  2:468703/1‑229 (MQ=255)        gTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATtacca                                                                                                                                                                                                                                                     <  1:468703/229‑1 (MQ=255)                      aaTTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCTCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACATCGATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTcac                                                                                                                                                                                                                 <  1:514532/250‑1 (MQ=255)                                         catccGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGAt                                                                                                                                                                                             >  2:149584/1‑251 (MQ=255)                                              ggggTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGAt                                                                                                                                                                                                      >  1:138314/1‑237 (MQ=255)                                              ggggTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGAt                                                                                                                                                                                                      <  2:138314/237‑1 (MQ=255)                                               gggTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGCTTGATAc                                                                                                                                                                                       >  1:40082/1‑251 (MQ=255)                                               gggTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATAc                                                                                                                                                                                       <  2:165372/251‑1 (MQ=255)                                                                          ggCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATccg                                                                                                                                                            <  2:143977/251‑1 (MQ=255)                                                                              caatcatgcatCAAAAAAGATGTGAACTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTGAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATccgcc                                                                                                                                                          <  2:431620/245‑1 (MQ=255)                                                                                   atgcatCAAAAAAGCTGTGAGCTTGATCCAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACATATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGAACCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCggca                                                                                                                                                    >  2:338454/1‑248 (MQ=255)                                                                                                       gCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGtt                                                                                                                               >  1:193054/1‑251 (MQ=255)                                                                                                           taTCAAAAACAAAAAATATTTCACTCGACTGGAGTATTTATATTGCACCCGTTTGGTCGGTTTCGACTGTAAATCAGAAAGGAGAAAACACCTTTGACGACCTACGGTCGTACCCGTATCGCAATTACCTCTGGCAGCCGTGTTAGTGGT‑AGGCGCACCGGTCATAGTTGCAATTTCCTGTCGAGTGATACTGAAGGGCTGACCGGTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTtgtg                                                                                                                            <  1:544772/249‑1 (MQ=255)                                                                                                                 aaacaaaaaATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTg                                                                                                                                                                                                                                                  <  1:255761/127‑1 (MQ=255)                                                                                                                 aaacaaaaaATATTTCAATCGCCAGGAGTGTTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTg                                                                                                                                                                                                                                                  >  2:255761/1‑127 (MQ=255)                                                                                                                    caaaaaATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGAGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGCGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACt                                                                                                                  >  2:433340/1‑251 (MQ=255)                                                                                                                         aaTATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGACAACACCTATGACGACCTACGATCGTAACCGTAACGCACTTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGcac                                                                                                             >  1:505661/1‑251 (MQ=255)                                                                                                                                cACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTgg                                                                                                       >  2:371069/1‑250 (MQ=255)                                                                                                                                     gACAGGAGTATTTATATTGCGCACGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCAGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCCACACTGGACCTg                                                                                                  >  1:162064/1‑250 (MQ=255)                                                                                                                                              aTTTATATTTCACCCGTTACGTGGTCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTTCGATCGTAACCGTAACGCAATTACCACTGTCAGCCGTGTTA‑TGTTTAGCGGCACCGGTCACACTGGCAAGATCTTGTCGATTGATACTGAATGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGGCCTGATTCGtctc                                                                                         <  1:285379/250‑1 (MQ=255)                                                                                                                                                       gcgcCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACt                                                                               <  1:149584/251‑1 (MQ=255)                                                                                                                                                           ccGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACCCCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTTGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTTATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGc                                                                           <  1:107947/251‑1 (MQ=255)                                                                                                                                                            cGTTACGTGGGCTTCGCCTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCg                                                                          >  1:476444/1‑251 (MQ=255)                                                                                                                                                               tACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTc                                                                                                                                                                                                <  2:365495/130‑1 (MQ=255)                                                                                                                                                               tACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTc                                                                                                                                                                                                >  1:365495/1‑130 (MQ=255)                                                                                                                                                                                      aGAAAGGAGAAAACCCCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATgc                                                                 <  2:415867/234‑1 (MQ=255)                                                                                                                                                                                      aGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATgc                                                                 >  1:415867/1‑234 (MQ=255)                                                                                                                                                                                       gAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGAAATTACCACTGGCAGCCGTGTTA‑TGGTTAGAGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACAGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCCCCACTGGACCTGATTAGTCTCGGCATGACCTAAGCGGGTGAATGCAGCCGATGGCGGCATTgg                                               >  1:421688/1‑250 (MQ=255)                                                                                                                                                                                          aGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGAACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCttt                                            >  1:227697/1‑251 (MQ=255)                                                                                                                                                                                                  accccTAGGACGACTTACGAACGAACCCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCCCACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTc                                    <  2:193763/248‑1 (MQ=255)                                                                                                                                                                                                              cccTACGACCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACGTCACGGAATAtttt                        <  2:260527/250‑1 (MQ=255)                                                                                                                                                                                                                                aacGCAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgcg      >  1:387790/1‑251 (MQ=255)                                                                                                                                                                                                                                    cAATTACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCCCCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAt  >  2:276129/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        GCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTA‑TGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAT  >  NC_000913/1629016‑1629493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑