Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,037	A→G	I518I (ATT→ATC)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,037	0	A	G	100.0%	97.9 / NA	27	I518I (ATT→ATC)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/15);  total (12/15)

CGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCG  >  NC_000913/1540802‑1541272                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            cGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGTCGCGGc                                                                                                                                                                                                                              <  1:107891/251‑1 (MQ=255) cGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCgg                                                                                                                                                                                                                               <  1:152238/250‑1 (MQ=255)                     aaCCAGCCGGTTTGGGGGCGCAGTTTTCCCTGGCCGACATAGTGTGCCCAGCCGCCCCCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCAGCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGGTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTTTCGCGGCACGCCAGTAATTTGCTCAcc                                                                                                                                                                                                          <  2:471786/251‑1 (MQ=255)                            cGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCt                                                                                                                                                                                                   <  1:14424/251‑1 (MQ=255)                                       cgcAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCTATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACgg                                                                                                                                                                                        <  1:24041/251‑1 (MQ=255)                                          aGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGttt                                                                                                                                                                                     <  1:522369/251‑1 (MQ=255)                                                       cGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACGCCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTa                                                                                                                                                                         >  2:501966/1‑250 (MQ=255)                                                        gACATAGTGTGCCCAGCCGCCACCTCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTGCGATAGACTGGCGCGGCACGCCAGTAAGTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAAt                                                                                                                                                                       <  1:519768/251‑1 (MQ=255)                                                            aaGTGTCCCCAGCCGCCACCGCTTTGCCCGACCCAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAAGCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCttt                                                                                                                                                                   <  2:533178/250‑1 (MQ=255)                                                             aGTGTGCCCAGCCCCCACCGCTGTGCCCGACACAGCCGCAGAAGATCAGCATATTGATCACCCCGCGGTAGTTCATGTCCATGTGAGACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCTTCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTGTCGATAGACTTGCGCGGCACGCCAGTGATTTGCTCTCCCCAGGCTGTTGTTTATGGTTTGATCTCattgaaatcttt                                                                                                                                                                   <  2:88941/250‑12 (MQ=255)                                                              gtgtGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCg                                                                                                                                                                 <  1:185933/251‑1 (MQ=255)                                                                        ccgccACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTc                                                                                                                                                       <  1:113763/251‑1 (MQ=255)                                                                           ccACCGCTCTGCCCGCCAAAACCGAAAAAGATCAGCATATTGATCATCACGCGGCAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCAAGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCACAGGCTGGGGCGTACGGTTTGACTTCAGCGTAATCTTTCGCACTGTTTTCATc                                                                                                                                                    >  2:66582/1‑251 (MQ=255)                                                                              ccGCTTTGCCCGTCACACCCGCAGAAGATCAGCATATTGATCCTCCCGCGGTAGTTCATGTCCTTGTGATACCTGTGGTTAACACCTGCGTCGAGGATAATCTTCGAGCGCCCTTGCGTTTTTTGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGAGATATGGGCGCGGTACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCTGCGTAATTTTTCGCACTGTGTTCATCTTc                                                                                                                                                 <  2:520945/251‑1 (MQ=255)                                                                              ccGCTTTGCCCGACACACCCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTc                                                                                                                                                 >  1:173126/1‑251 (MQ=255)                                                                                            acaACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTTGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGa                                                                                                                                   <  1:44885/251‑1 (MQ=255)                                                                                                                     atcataCCGCGGTAGTTCAAGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGTATAATCATCGAGCGCCCATGCGTTTTATGGTGGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATGTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATc                                                                                                          <  2:5958/251‑1 (MQ=255)                                                                                                                                            cATGTGATACCAGTGGTTAACACCTGCGCAGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAgg                                                                                   >  2:521701/1‑251 (MQ=255)                                                                                                                                                     ccAGTGGTTAACACCTGCGACGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCCAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGCCAGGTGTTGCCAt                                                                          >  2:347475/1‑251 (MQ=255)                                                                                                                                                          ggTTAACACCTACGCCGAGGATACTCATCGAGCGCCCATGCGCTTTCTGGGCGGTATCGGCAAATTCACGGGCGCTGGTTTAGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATACACGCTGACCACCGGACCGGTGTTGCCCTCGACt                                                                     >  2:80399/1‑251 (MQ=255)                                                                                                                                                           gTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACt                                                                     >  1:77432/1‑250 (MQ=255)                                                                                                                                                                       gcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCCGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGtttt                                                        >  1:362611/1‑251 (MQ=255)                                                                                                                                                                                            cccATGCGTTTTATGGGAGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCAcc                                    >  1:440809/1‑250 (MQ=255)                                                                                                                                                                                                           ggCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACCGGGTTTGCCATCGACTAACGTCAGGTTTTTAACGGGAAATTGGCGCCCCAGCACCGGATTGTGtt                    >  2:522155/1‑251 (MQ=255)                                                                                                                                                                                                                   cGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCt             <  2:173126/250‑1 (MQ=255)                                                                                                                                                                                                                      cAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCg          >  1:8074/1‑250 (MQ=255)                                                                                                                                                                                                                             aCGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCg  >  1:292768/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            CGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCG  >  NC_000913/1540802‑1541272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑