Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,758	G→A	intergenic (‑7/‑221)	mcaS ← / → smrA	small regulatory RNA McaS/DNA endonuclease SmrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,758	0	G	A	100.0%	66.6 / NA	19	intergenic (‑7/‑221)	mcaS/smrA	small regulatory RNA McaS/DNA endonuclease SmrA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/11);  total (8/11)

GAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTG  >  NC_000913/1405533‑1405988                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       gAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTg                                                                                                                                                                                                               <  1:269278/251‑1 (MQ=255)        tACCGAAAGCGGCCTATTATTTGATTCCGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTGAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCGACAGTACACACAGCAGTGCATCCGCTTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTGTCCTTTAGTTGCCAAtt                                                                                                                                                                                                         <  2:219584/250‑1 (MQ=255)              aaGCGGCCTATTATTTGATTCAGCGGCGTGATAGGCGCCAGACACCACTGACCGCGTCATTAAGCACGCAATGCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCTTCTTAAATCCGGCATTGTCTCCGCTGCGCCGGTGACTGTATTATAACGGTTTTAGCCTTTAGGTGCCAATTTTCTTCa                                                                                                                                                                                                  <  2:51936/251‑1 (MQ=255)                                              aggcGCCAGCCTCCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATATAGTCGGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCGCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTGCTTCAGTGACAGATTTCACGAAAATATAAGTTACat                                                                                                                                                                   <  2:178470/247‑1 (MQ=255)                                                       acacCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTTCATTATTAATATc                                                                                                                                                         <  1:172964/251‑1 (MQ=255)                                                               gACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATGATTAATATCGtgaatg                                                                                                                                                  <  1:395805/250‑1 (MQ=255)                                                                               aCGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACATATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGt                                                                                                                                 <  2:339251/251‑1 (MQ=255)                                                                                                        aTCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCATACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTc                                                                                                                                                                                      >  1:296332/1‑173 (MQ=255)                                                                                                        aTCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAat                                                                                                          <  1:26900/249‑1 (MQ=255)                                                                                                                 gTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACtt                                                                                               >  2:304207/1‑251 (MQ=255)                                                                                                                        cTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTg                                                                                        >  2:162051/1‑251 (MQ=255)                                                                                                                                     tAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTa                                                                            >  1:10656/1‑250 (MQ=255)                                                                                                                                          tCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACgt                                                                      >  1:192927/1‑251 (MQ=255)                                                                                                                                               cGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGttgttg                                                                 <  1:515298/251‑1 (MQ=255)                                                                                                                                                 aCATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTTCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTgg                                                                >  1:144015/1‑250 (MQ=255)                                                                                                                                                     ccGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGgaccg                                                            >  1:500914/1‑250 (MQ=255)                                                                                                                                                                                gTGCCTCCGCGTCTTAAATCCGGCATTGTCTCCTCGGCGCCGGTGACTGTATTATAACGTTTTTAACCTTTAGTTGCCAATTTTCGTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTTCGTAAAATAGCGGCAATATTTGGAGCTTATTACGGGAAATGTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGctct                                <  2:223218/251‑1 (MQ=255)                                                                                                                                                                                          gTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCggg                      <  2:410661/251‑1 (MQ=255)                                                                                                                                                                                                               cctcTGCGCCGGTGACTGTATTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTCATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTg  >  1:85396/1‑250 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       GAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACAGATTTCACGAAAATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTG  >  NC_000913/1405533‑1405988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑