BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,535	T→G	A445A (GCT→GCG)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,535	0	T	G	99.3%	477.3 / NA	145	A445A (GCT→GCG)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (60/84); total (60/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 145 total aligned reads displayed.

GCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCA  >  NC_000913/284288‑284776

gcggcgTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGGGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCGAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCg                                                                                                                                                                                                                                                <  1:512951/251‑1 (MQ=255)

 gggcgTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCgg                                                                                                                                                                                                                                               <  1:180742/250‑1 (MQ=255)

  ggcgTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCgg                                                                                                                                                                                                                                               >  1:63224/1‑250 (MQ=255)

  ggcgTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGGGACCGGCCAGACGGTGGGCGAGAACCTTGAAGGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGa                                                                                                                                                                                                                                              <  2:251882/251‑1 (MQ=255)

     gTGCCGGCGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAgg                                                                                                                                                                                                                                           >  1:124503/1‑251 (MQ=255)

     gTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAgg                                                                                                                                                                                                                                           <  1:428893/251‑1 (MQ=255)

       gCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGGCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGacggat                                                                                                                                                                                                                                         >  2:49812/1‑246 (MQ=255)

         cGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGCAGGTTCg                                                                                                                                                                                                                                       >  1:130277/1‑251 (MQ=255)

           gAGGTGATGCTCCACCTGCGCGACCGCGGCCGGGTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGt                                                                                                                                                                                                                                     <  2:136552/251‑1 (MQ=255)

            gggggatgccccgcccgcgcGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGTCCAGACGGTGGGCGAGAACCTGGAAGGGTGGCAGGCGGCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCGCGACGGTCTGCTTCCCGTCGGGCAACATCGCGCGGGAAggggcggtg                                                                                                                                                                                                                                    <  2:360647/235‑8 (MQ=255)

            aGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGCCCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGCGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTg                                                                                                                                                                                                                                    >  1:489005/1‑251 (MQ=255)

             agTGATGCTCCACCTGCGCGACCTCGGCCGGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGAGGACGTGATCC‑TGCCGCCTGAGAAGGCAAAAGCGAAAGGGCTTACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGa                                                                                                                                                                                                                                   <  1:258708/250‑1 (MQ=255)

              gTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGGGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGAAGGACGGCGTAGAGCCGGATGACGGGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGCCGGTCTGCTTCCCGACGGGCACCATCGCGCCGGAAGGTTCGGTggt                                                                                                                                                                                                                                  >  1:333909/1‑249 (MQ=255)

               tGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATc                                                                                                                                                                                                                                 >  1:416226/1‑251 (MQ=255)

               ggATGCTCCCCCTGCGCGACCTCGGCCTGGTGCATCTGGACGCCATGACCGGGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCGGGGCGCTCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTTGCCTCGACGGTCTGCTTCCCGACGGGCATCATCGCGCCGGAAGGTTCGTTGATc                                                                                                                                                                                                                                 <  2:498161/250‑1 (MQ=255)

                gATGCTCCCCCTGCGCGACCTCGGCCTGCTGCAGCTGGACGCCAGGACCGTGACCGGCCAGACGGTGGGCGAGACCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCa                                                                                                                                                                                                                                <  2:40152/251‑1 (MQ=255)

                   gCTCCCCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAgg                                                                                                                                                                                                                             <  1:326861/251‑1 (MQ=255)

                      ccgccggcgcgcCCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCGGCCGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCa                                                                                                                                                                                                                          <  2:216430/245‑1 (MQ=255)

                      ccgccTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGc                                                                                                                                                                                                                            <  1:178562/246‑1 (MQ=255)

                        cccTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACg                                                                                                                                                                                                                        <  2:416226/250‑1 (MQ=255)

                        aCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGCAGGTTCGGTGATCAAGGCCACg                                                                                                                                                                                                                        >  1:326382/1‑251 (MQ=255)

                           ggcgcGACCTCGGCCTGCGGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGTGCCGGAAGGTTCGGTGATCAAGGCCACGGCg                                                                                                                                                                                                                     <  1:182038/250‑1 (MQ=255)

                            gcgcGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGc                                                                                                                                                                                                                      <  1:310680/249‑1 (MQ=255)

                              gcgACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATc                                                                                                                                                                                                                  <  1:549848/251‑1 (MQ=255)

                              gcgACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATc                                                                                                                                                                                                                  <  1:1473/251‑1 (MQ=255)

                                      ggCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTc                                                                                                                                                                                                          <  2:117954/251‑1 (MQ=255)

                                        ccTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCgg                                                                                                                                                                                                        >  2:271933/1‑251 (MQ=255)

                                         ctgctgCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggt                                                                                                                                                                                                       >  1:321516/1‑251 (MQ=255)

                                           gctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggt                                                                                                                                                                                                       <  2:31495/249‑1 (MQ=255)

                                           gctgcATCTGGACGCCATCGCCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGAAGGCGTCCGAGCGCCGTGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGTCCCGTCggt                                                                                                                                                                                                       <  2:443466/249‑1 (MQ=255)

                                           gcggcATCGGGACGCCCGGACCGGGACCGGCCCGACGGGGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggt                                                                                                                                                                                                       <  2:384080/246‑1 (MQ=255)

                                             tgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGgtggtg                                                                                                                                                                                                   >  1:489478/1‑251 (MQ=255)

                                                           aTGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGt                                                                                                                                                                                     <  1:546538/251‑1 (MQ=255)

                                                             gaccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGtat                                                                                                                                                                                   <  1:205041/251‑1 (MQ=255)

                                                              accgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGtata                                                                                                                                                                                  <  1:152015/251‑1 (MQ=255)

                                                                    cccggccagagtggggggtagaACCTTGAAGGGTGGCGTGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCGGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGACCAAGGCCACTGCGATCGACCCGTCGGTGGTGGGCGAAGAGGGCGTATAccacca                                                                                                                                                                            <  2:530102/232‑1 (MQ=255)

                                                                      ggtccgcaCGGTGGGCGTCAGCCTTGAATGCGGGCCGGCTCCCGAGCGCCGGGCGGGCTTACGCCATTGCCGGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCGGCCGGAGAAGCCAAAAGCGAAAGGGCTGACCTCGACGGTCGGCTTCCCGCCGGGCTACATCGCGCCGTAAGGTTCGGTGATCAAGACCACGGCGATCGCCCCGTCGGTGGTTGGCGAAGGTGGCGGATacctcctaa                                                                                                                                                                          <  2:179464/244‑7 (MQ=255)

                                                                      cgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGCAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACcaccac                                                                                                                                                                           >  1:260759/1‑250 (MQ=255)

                                                                      cgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACcaca                                                                                                                                                                          <  1:100416/251‑1 (MQ=255)

                                                                             gcGGTGGGCGAGAACCTTGAAGGGGGGCAGGCGGCGGGGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACAccggccg                                                                                                                                                                   <  2:155405/250‑1 (MQ=255)

                                                                                gTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggt                                                                                                                                                                <  2:349406/251‑1 (MQ=255)

                                                                                   ggCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcg                                                                                                                                                             <  1:438117/251‑1 (MQ=255)

                                                                                    gCGAAAACCGTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgg                                                                                                                                                            <  2:350777/251‑1 (MQ=255)

                                                                                      gagaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgggg                                                                                                                                                          >  2:444997/1‑250 (MQ=255)

                                                                                         aaCCTTGAATGGGGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGtt                                                                                                                                                       <  2:211441/251‑1 (MQ=255)

                                                                                              tGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGtct                                                                                                                                                  >  1:124635/1‑251 (MQ=255)

                                                                                              ggAATGGTGGCAGGCGCCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGt                                                                                                                                                    <  2:152703/248‑1 (MQ=255)

                                                                                                 aTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGACGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGGGCGGGTGTTTGTCTCgg                                                                                                                                               >  1:215045/1‑251 (MQ=255)

                                                                                                     tggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgc                                                                                                                                           >  1:540013/1‑251 (MQ=255)

                                                                                                        cAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgcgc                                                                                                                                         >  1:507025/1‑250 (MQ=255)

                                                                                                          ggCGCCCGCGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTGCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCCCGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCagg                                                                                                                                      <  2:24009/251‑1 (MQ=255)

                                                                                                            cGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcg                                                                                                                                    <  2:63224/251‑1 (MQ=255)

                                                                                                              cccgggcgcCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgat                                                                                                                                  <  2:333909/246‑1 (MQ=255)

                                                                                                               cctaGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGCCGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGGGCGGGTGTTTGTCTCGGAAGCGCaggcgatc                                                                                                                                 >  2:422373/4‑251 (MQ=255)

                                                                                                                cgcgcgcCGGGCGCGCTTCCGCCAGTGCCTTCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatca                                                                                                                                <  2:514339/248‑1 (MQ=255)

                                                                                                                cGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatc                                                                                                                                 >  1:550640/1‑250 (MQ=255)

                                                                                                                    cgcCGGGCGCGCTCCCGCCCGTGCCTGCGCGAGCAGGGCGGCGTAGAGCCGGATGTCGTGCTCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGCCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggc                                                                                                                            <  1:464615/251‑1 (MQ=255)

                                                                                                                     gcCGGGCGCGCTTCCGCCAGTGCCTGCGCGATGAGGACGGATTAGCGCCGGATGACGTGATCC‑TGCCGCCGGCGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTCCCCGACGTGCTACATCGCGCCTGAAGGTTCGGTGATCAAGGCCGCGTCGAGCGTCCCGTCGGTTGTGGGCGAAGATGGCGTATACCTCCTCACAGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcg                                                                                                                           <  1:27688/251‑1 (MQ=255)

                                                                                                                       cGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGCACGGGCTGACCTCGACGGTCTGCTTCCCGATGGGCAACATCGCGCCGGAAGGTTCGGCGATCAAGGCCACGGCGATCGACCCGTCGGTGGGGGGCGAAGATGGCGTATACCACCCCACCCGCCGGGTGCGGGGGTTTGTCTCGGCAGCGCaggcgatccaggcgat                                                                                                                         >  2:472258/1‑251 (MQ=255)

                                                                                                                          gcgcgtgtCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGTAAGGTTCGGTGATCAAGGCCACGGCGAGCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGTCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAggcgatcaaggcgatcaa                                                                                                                      <  2:540013/244‑1 (MQ=255)

                                                                                                                             cgcTGCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCTCGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGgcgatcaaggcgatcaag                                                                                                                     <  1:287097/249‑1 (MQ=255)

                                                                                                                              gcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCgg                                                                                                                  >  1:337567/1‑251 (MQ=255)

                                                                                                                              gcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCgg                                                                                                                  >  2:185411/1‑251 (MQ=255)

                                                                                                                              gcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCAGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAACAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGGGGGCGAGGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCgg                                                                                                                  >  2:266779/1‑251 (MQ=255)

                                                                                                                                       ggtgcgtgcGCGAGCAGGACGGCGTAGGGCCGGATGACGGGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTGTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCTGGAAGAGAtt                                                                                                         <  2:341123/250‑1 (MQ=255)

                                                                                                                                       aGTGCCTGCGCGAGCTGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTGCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCCCCACACCGGCCGGGTGCGGGGGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGAtt                                                                                                         <  1:495356/251‑1 (MQ=255)

                                                                                                                                              gcgcGAGCAGGACGGCGTAGAGCCGGAAGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCa                                                                                                    <  1:305969/249‑1 (MQ=255)

                                                                                                                                                 cgAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGCTCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCg                                                                                               >  2:216653/1‑251 (MQ=255)

                                                                                                                                                 cgAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCg                                                                                               <  1:389923/251‑1 (MQ=255)

                                                                                                                                                   aGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGat                                                                                             <  1:407593/251‑1 (MQ=255)

                                                                                                                                                       ggACGGCGTAGAGCCGGCTGACGTGATCC‑TGCCGCCGGAGCAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCCACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGGGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGGGTTTGTCTCGGAAGCGCAGGCGCTCAAGGCGATCAAGCGGGAAGAGCTTGTGCAGGGCGATATc                                                                                          >  2:278534/1‑250 (MQ=255)

                                                                                                                                                           ggCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtggt                                                                                     >  1:290270/1‑251 (MQ=255)

                                                                                                                                                              gaaaagCCGGAAGACGGGATCCGTGC‑GCAGGAGAAGGCAAAATCGAAAGGGCTGAACTCGACGGTAGGCTTCCAGACGGACAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTAGACCACCAAACCGGTCGGGTGGGGGGGTGTGTCTCGGAAGCGGAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGACGATATCAtggttgt                                                                                  <  2:29472/247‑4 (MQ=255)

                                                                                                                                                                    ccGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATcgg                                                                            >  1:385234/1‑251 (MQ=255)

                                                                                                                                                                      ggATGCCGTGATCC‑TGCGGCCGGAAAACGAAAATGCGAACGGGATGACCTCCACGGTATGCTTCCCGACGGACAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGAGGTGGGCGAAGATGGCGTATACCCCCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGACCAAGCGGGAAGCGATTGTGCAGGGCGATATCATGGTGGTGATcggcg                                                                          >  2:38065/1‑251 (MQ=255)

                                                                                                                                                                          gACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATcggcggcgg                                                                      >  1:368263/1‑251 (MQ=255)

                                                                                                                                                                          gACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATcggcggcgg                                                                      <  1:513942/251‑1 (MQ=255)

                                                                                                                                                                           aCGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGAccc                                                                                                                                                                                                             <  1:53963/115‑1 (MQ=255)

                                                                                                                                                                               gATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGGTATCATGGTGGGGATCGGCGGCGGGCCgg                                                                 >  2:3897/1‑250 (MQ=255)

                                                                                                                                                                               gATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGATGACCTCGCCGGTATGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGAGCAGGGCGATCTCATGGTGGTGATCGGAGGCGGGCCGt                                                                 >  1:415113/1‑251 (MQ=255)

                                                                                                                                                                                  cc‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGAGATCATGGTGGTGATCGGCGGCGGGCCGTccg                                                              <  1:452792/251‑1 (MQ=255)

                                                                                                                                                                                        cgccgGAGAAGGCAAAAGCGAAAGGGTCGCCCCCGTCGTTCTCCTCCCCGACGGGCAACCTCGCGTCGGAAGCTTCCGTGATCAAGGCCACGTCGATCGACCCGTCGGCGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCCAGCGGGAAGCGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccggcacc                                                         <  1:651/251‑1 (MQ=255)

                                                                                                                                                                                        cgccgGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccggcacc                                                         >  1:235842/1‑251 (MQ=255)

                                                                                                                                                                                        cgccgGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccggcacc                                                         <  2:356957/251‑1 (MQ=255)

                                                                                                                                                                                            ggAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccg                                                              <  2:129012/242‑1 (MQ=255)

                                                                                                                                                                                            ggAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccg                                                              >  1:129012/1‑242 (MQ=255)

                                                                                                                                                                                             gagaAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCAt                                                    >  1:473480/1‑251 (MQ=255)

                                                                                                                                                                                              agaAGGCAAAGGCGAAAGGGCGGACCTCGACGGGCTGCTCCCCGACGGGCAACGTCGCGCCGGAAGTTTCGGTGATCAAGTCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGGATCCCACCACACCGGCCGTGTGCGGGTGTTTGTCGCGGAATCGCGGGCGATCTAGGCGATCAAGAGGGAAGAGAGTGTGCAGGGCGATATCATGGTTGTGATCGGCGGCGGGCCGTCCGGCACCTGCATg                                                   <  2:34445/251‑1 (MQ=255)

                                                                                                                                                                                                  ggCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGCCGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATggg                                                 >  2:444922/1‑248 (MQ=255)

                                                                                                                                                                                                     aaaaGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAag                                               >  2:358814/1‑248 (MQ=255)

                                                                                                                                                                                                       aaGCGAAAGGGCTGACCTCGACTGTCTGCTTCCCTACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGGGCAGGCGATCAAGGCGATCAAGCGGGAAGATATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGAcc                                          <  1:508370/251‑1 (MQ=255)

                                                                                                                                                                                                       aaGCGAAAGGGCGGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGAcc                                          <  2:331802/251‑1 (MQ=255)

                                                                                                                                                                                                             aaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTAcc                                      >  1:448936/1‑249 (MQ=255)

                                                                                                                                                                                                              aGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCTGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGc                                   <  1:91316/251‑1 (MQ=255)

                                                                                                                                                                                                                ggCTGACCTCGACGGTCTGCTCCCCGACGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGGCCACTGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATCCCACCACACCGGGCGGGTGCGGGTGTGTGGCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCTGCGGCGGGCCGTccggcacc                                                         <  1:197699/227‑1 (MQ=255)

                                                                                                                                                                                                                ggCTGACCTCGAAGGTCTGCTTCCCGCCGGGCAACCTCGCGCCGGCCGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGCTGGCGGGCGAAGATGGCGTCTACCACCAAACCGGCCGGGTGCGGGTGTTTGTCTCGGGAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGCGATTGTGCCGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccgggaccagaacgggaaagcggcggtaaggg                                 >  2:197699/1‑227 (MQ=255)

                                                                                                                                                                                                                                     tCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCata              <  1:3897/249‑1 (MQ=255)

                                                                                                                                                                                                                                      cccGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGACCACCCCCGCGCTAAAGCATAtcc           >  2:479583/1‑250 (MQ=255)

                                                                                                                                                                                                                                              gCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGc   <  2:205183/251‑1 (MQ=255)

                                                                                                                                                                                                                                                 aCATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATG‑AAGGGACCTACCCGCTCAACCCCGCGCTCAAGCAAATCTCGGGGGGCa  >  1:273453/1‑248 (MQ=255)

GCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCC‑TGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCA  >  NC_000913/284288‑284776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`