Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,395,367	G→A	R186C (CGT→TGT)	ynaI ←	small conductance mechanosensitive channel YnaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,395,367	0	G	A	100.0%	85.5 / NA	25	R186C (CGT→TGT)	ynaI	small conductance mechanosensitive channel YnaI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/16);  total (9/16)

CGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTT  >  NC_000913/1395125‑1395604                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              cGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGt                                                                                                                                                                                                                                       <  1:384841/251‑1 (MQ=255)           ttCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTGTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATAc                                                                                                                                                                                                                              <  2:272769/250‑1 (MQ=255)            tCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACt                                                                                                                                                                                                                             <  1:301949/250‑1 (MQ=255)                     cACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACgg                                                                                                                                                                                                                   <  1:110044/251‑1 (MQ=255)                      aCGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGGTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCCATACTGAAAGGACGGt                                                                                                                                                                                                                  >  1:364194/1‑251 (MQ=255)                        gTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCg                                                                                                                                                                                                                <  1:205501/251‑1 (MQ=255)                                                       cGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCTGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTAc                                                                                                                                                                                 <  1:315982/251‑1 (MQ=255)                                                                                                            gTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCaa                                                                                                                              >  2:90148/1‑249 (MQ=255)                                                                                                               cTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTc                                                                                                                         >  1:134491/1‑251 (MQ=255)                                                                                                                        tttcgcggacggacgaAAAAAGCGGGTTCGGTACGTGCAATGGGCGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTGCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATAATGAAAGGACGGTCGAAATAGAACATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTCCCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGcc                                                                                                                 <  2:189643/239‑1 (MQ=255)                                                                                                                               gATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGc                                                                                                         >  1:49040/1‑251 (MQ=255)                                                                                                                                              cGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCg                                                                                                                                                                >  1:227972/1‑181 (MQ=255)                                                                                                                                              cGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTATACCg                                                                                                                                                                <  2:227972/181‑1 (MQ=255)                                                                                                                                                              aaTGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAAc                                                                          <  2:364194/251‑1 (MQ=255)                                                                                                                                                                 ggACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAg                                                                       <  2:49040/251‑1 (MQ=255)                                                                                                                                                                  gACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGc                                                                      <  1:347235/251‑1 (MQ=255)                                                                                                                                                                                  cGTAATTTTGTTAAGTCGCCAGCCAATGTCCGCTACTGTACCTTCGATAGTGCTGTCCGGTGAACAGATCCAGTCGCCAAGACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACACAGAATATCTTTACCGGCCATACCGACAGCCAGACCTCCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCTAAATGTTCGCCATAAAGCATAACTAGCACCACAAGAATgttg                                                       <  2:96596/250‑4 (MQ=255)                                                                                                                                                                                  cGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTg                                                       >  2:422027/1‑250 (MQ=255)                                                                                                                                                                                      aTTTTGGTAATTCGCCAGCCAATGTCCCCTACTGTTCCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCATCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCa                                                                                     <  1:289726/216‑1 (MQ=255)                                                                                                                                                                                        tttGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATa                                                                                                                                                                                                    <  1:9284/103‑1 (MQ=255)                                                                                                                                                                                        tttGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATa                                                                                                                                                                                                    >  2:9284/1‑103 (MQ=255)                                                                                                                                                                                          tGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTtgat                                               <  1:414083/250‑1 (MQ=255)                                                                                                                                                                                                                             tCGATATGTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATcgc            <  2:399112/250‑1 (MQ=255)                                                                                                                                                                                                                              cGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTTGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGCATATCTTTACCGGCCATACAGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGAGATTTTGATGATACGCGACATAATACGCGCACTGGTGATATcg             >  1:256949/1‑248 (MQ=255)                                                                                                                                                                                                                                      cTGTCCGGTGAACAGATACAGTCGCCAATCCTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGGGATTTTGATGAAACGCGACATAATCCCCGCACTGGTGATATCGCGACCtttttt  >  1:149862/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              CGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTT  >  NC_000913/1395125‑1395604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑