Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,684	T→C	intergenic (‑39/‑104)	clsA ← / → yciY	cardiolipin synthase A/uncharacterized protein YciY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,684	0	T	C	100.0%	35.7 / NA	12	intergenic (‑39/‑104)	clsA/yciY	cardiolipin synthase A/uncharacterized protein YciY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/4);  total (8/4)

AACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTTACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAGTGCATTC  >  NC_000913/1308500‑1308914                                                                                                                                                                                         |                                                                                                                                                                                                                                       aaCGGCAGAATGTAACTAATCAGCAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAACGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCCTAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCgggggtggggg                                                                                                                                                                      >  2:205117/1‑251 (MQ=255)      cAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGtata                                                                                                                                                                  >  2:399109/1‑250 (MQ=255)            gTAAATAACCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCATCTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTTAAAGCGCATGGTTATGAGTTCACGCGTATGGTTGCTGTTGGCAAATAAAAACGCGTGAAAATCACTGCTCAGTGTCGGGGGTGGGTGTATAATGAcc                                                                                                                                                            <  2:280055/250‑1 (MQ=255)                             aGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCAt                                                                                                                                             >  1:279328/1‑248 (MQ=255)                                        gAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGAGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACAt                                                                                                                               >  2:168691/1‑251 (MQ=255)                                                                      cGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTg                                                                                                 >  1:528528/1‑251 (MQ=255)                                                                                                                                                  tAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCcac                     >  1:458432/1‑251 (MQ=255)                                                                                                                                                    gATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCcac                     >  1:212936/1‑249 (MQ=255)                                                                                                                                                            aTCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGa           <  1:381987/251‑1 (MQ=255)                                                                                                                                                               cTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAgg        >  2:532802/1‑250 (MQ=255)                                                                                                                                                                 tttGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACGGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAGt        <  2:297594/249‑1 (MQ=255)                                                                                                                                                                     aaaGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAGTGCATTc  <  1:255823/251‑1 (MQ=255)                                                                                                                                                                                         |                                                                                                                                                                                                                                       AACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTTACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAGTGCATTC  >  NC_000913/1308500‑1308914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑