Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	69.5 / NA	23	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/14);  total (9/14)

GCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAAC  >  NC_000913/1306490‑1306970                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           gCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACCTCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCg                                                                                                                                                                                                                                         <  2:415767/250‑1 (MQ=255)            ggggTCCTATGATGAGGTCTACCACAATCCACTACATCCTTACCCCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCACCCAG‑TGACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTGGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTGCTTGCCTGa                                                                                                                                                                                                                             <  1:174408/251‑1 (MQ=255)                aCCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGt                                                                                                                                                                                                                         >  1:137456/1‑251 (MQ=255)                 ccTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGAGCAACCCGCGTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTc                                                                                                                                                                                                                        <  1:512940/251‑1 (MQ=255)                              cTACCACAATCCCCTCCACCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGAACCGGATCTGGAGAAGAACAAAACCATCCAG‑TTCCTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCttt                                                                                                                                                                                                              <  2:307836/248‑1 (MQ=255)                              cTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCttt                                                                                                                                                                                                              >  1:307836/1‑248 (MQ=255)                                    caATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACACAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAAt                                                                                                                                                                                                     >  1:467344/1‑251 (MQ=255)                                                             gCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTTTCAGCCCttt                                                                                                                                                                            >  2:303838/1‑251 (MQ=255)                                                              cATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCtttt                                                                                                                                                                           <  1:259726/251‑1 (MQ=255)                                                                                     ccTGATCCGGAGCTGTAAACAAACAAAACCACCCAGGTTA‑GGGAAGGGGAATTACCGTCCCCGATCAACCCGCCTTCCGGTGGTGTTTTCCGTACCCGTGGCCCTATTGGCGGTCCAGAGTGCTCCAAAACAAGTCTTGGTCGTGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTGCACGCTAAAATCGAttatttat                                                                                                                                                    <  1:359140/251‑1 (MQ=255)                                                                                           ccGGATCTGGAGAAGAACAAAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGc                                                                                                                                              >  1:6962/1‑251 (MQ=255)                                                                                                ccTGGAGAAGACCAAACCCAGCCAG‑TTCCTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTTCCCGTTGCCCGATTGCCTGTCCAGAGTGCGCCAAAACACGTCCGGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTACTTAGCCCCGCCAgat                                                                                                                                         <  2:57429/250‑1 (MQ=255)                                                                                                        agaaCAAAACCATCCAG‑TTGCTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGGTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGATCTGGAGGGGAGTTTCAGACACGCCGTTTCGTGCCTGAAATTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATGCCCGCCAGATGATATGGc                                                                                                                                 <  2:488164/251‑1 (MQ=255)                                                                                                                       aG‑TTTCTGGAAGGGGAATTACCTCCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTTCGCCAAAACACGTCCTGTTCTGTAGGGCAGTTTCAGACACGCCGTTTCTTGCCTGAAAGGCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCTATTATTTATTCCCGCCAGATTATATGGCAAAGTTTGTGATCtt                                                                                                                  <  2:467344/251‑1 (MQ=255)                                                                                                                            aCTGGAAGGGGAATTCCCTTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTc                                                                                                              <  1:430300/251‑1 (MQ=255)                                                                                                                                gAAGGGGAATTGCCGGCGCAGATCAACCCGAATGCCGGGTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCATAGCAAGTCCTGGTCTGAAGGGGGGGTTCAAACACGCCGTTTCTTGCCTTAAAGACGATCCGCTTTAAAAACAATACGGGCAGACCGGTGGCGGCCCTTTTTCACGCTAAAAGCGACTATTTCTTCCCGCCCGCTGATATCGCAAAGTTTGTGCTCTTTTTCGCgg                                                                                                          >  2:71434/1‑251 (MQ=255)                                                                                                                                gAAGGGGAATTACCGTCGCCGAGCAACCCGCCTTCCGGTTGTGTTTTCCGTTCCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGAGCCGCTTTAAAATCAATGAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCTATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCgg                                                                                                          <  2:62825/251‑1 (MQ=255)                                                                                                                                 aaGGGGAATTACCTTCGCCGATCAACCCGCCTTCCGTTTGTGTGTTCCGTACCCGTGGCCCGATTGCCGGTCCAGAGGGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTGTCTTGCCTGAAAGTCGATCCGCGTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGata                                                                                                                                     <  2:489480/223‑1 (MQ=255)                                                                                                                                 aaGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGCAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTACAAGCGATTATTTATTCCCGCCAGATGata                                                                                                                                     >  1:489480/1‑223 (MQ=255)                                                                                                                                            ccttCGCCGATCAACCCGCCTTCCGGTTGTTGTTTCCGTCCCCGTGGCCCGATTGCCGGTCCAGATTTCGCCAAACCACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCTTTTCTTGCCTGAAATTCGATCCGCTTTAAAAACAATAAGGGCTGATAGTTGTCAGCCCTTTTTCACTCTAAAATCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATg                                                                                              <  2:331115/248‑1 (MQ=255)                                                                                                                                                 gCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGc                                                                                         >  1:11703/1‑251 (MQ=255)                                                                                                                                                           ccGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACCCGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCg                                                                               <  1:204936/251‑1 (MQ=255)                                                                                                                                                                                                                                         gggAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAAc  >  1:131378/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           GCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAG‑TTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAAC  >  NC_000913/1306490‑1306970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑