Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,9920AT96.9% 101.9 / NA 33N271Y (AAT→TAT) oppAoligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (15/16);  minor base G (0/1);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATA  >  NC_000913/1301760‑1302223
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       
gCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCAACAACGGTGCCTATACCTTAAAAGATTGTGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGTAAACCGTTATTAACCAGGTAACCTACTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCTCTACCGTAGTGGTGAAATCGACATGACGGAGAACAGCATGCCGATc                                                                                                                                                                                                                        <  2:229232/250‑1 (MQ=255)
    aaaGCCGCTATCGAGAAATTCGGCGAAAAAGGGACCCAGCCTGGTAATATCGTCGTCAACGTTGCCTATACCTTAAAAGATGGGGTCGTAAACGAACGTATCGTTCTGGAACGCAGCCCGACCTACAGGAAGAACGCGAAATCGGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGGAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAtt                                                                                                                                                                                                                   <  1:319375/251‑1 (MQ=255)
                gagaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCt                                                                                                                                                                                                       <  2:27306/251‑1 (MQ=255)
                 agaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTg                                                                                                                                                                                                      <  1:406739/251‑1 (MQ=255)
                        cGGCGAAAAATGGACCCAGCCTTGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTTGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCTATGTCATCCTCTACCGTAGTGCTGAAATCGTCATGACGTAGAACAGCATGCCTTTCTAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                                                                               <  2:533672/251‑1 (MQ=255)
                        cGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGCTTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTCTTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCg                                                                                                                                                                                                                       >  2:372059/1‑227 (MQ=255)
                        cGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                                                                               <  2:456484/251‑1 (MQ=255)
                        cGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGGAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCg                                                                                                                                                                                                                       <  1:372059/227‑1 (MQ=255)
                           cGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGAGCGAATTTTTCCAGAAGCTGAAAAAAgaga                                                                                                                                                                                            <  1:173289/251‑1 (MQ=255)
                                aaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCg                                                                                                                                                                                       <  1:152340/251‑1 (MQ=255)
                                                 aaTATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTGCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTGCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTGACGTTg                                                                                                                                                                       <  2:493182/250‑1 (MQ=255)
                                                            cAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtgt                                                                                                                                                           >  1:141535/1‑251 (MQ=255)
                                                                       ataCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTAt                                                                                                                                                >  1:379234/1‑251 (MQ=255)
                                                                         aCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTAtt                                                                                                                                               <  1:320512/250‑1 (MQ=255)
                                                                                   aTTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaac                                                                                                                                    >  1:115274/1‑251 (MQ=255)
                                                                                                cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGCAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGTAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGCAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAAAAACCGc                                                                                                                       >  1:286455/1‑251 (MQ=255)
                                                                                                           ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCTTTAGTAACCATTGAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTTCCGTATTAGTGAAAGCTACATGACGTAGAACAGCATGCCTAGCGAATTGTTCCAg                                                                                                                                                                                                            <  2:39135/155‑1 (MQ=255)
                                                                                                           ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCATATTGCTTCTGAAGTTACCGCTGTCAACCGCTACAGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGCATTGTTCCAg                                                                                                                                                                                                            >  1:39135/1‑155 (MQ=255)
                                                                                                                         ccGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaa                                                                                                                                                                                                   >  1:239818/1‑150 (MQ=255)
                                                                                                                         ccGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaa                                                                                                                                                                                                   <  2:239818/150‑1 (MQ=255)
                                                                                                                                                         taACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAgaga                                                                                                                                                                                            <  2:15375/125‑1 (MQ=255)
                                                                                                                                                         taACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAgaga                                                                                                                                                                                            >  1:15375/1‑125 (MQ=255)
                                                                                                                                                           accaggcaacaaatttgaatATTGCTGCTGAAGTTACGGATGTCAACCGCAACCGTAGTGGTGAAATCGACATGACGACTAACAGCATGACGATCGACTTGTTCCCGAAGCTGAACACAGAGATCCCGGCCGAAGTTCACGTTGATCCATACCTGTGCCCTTACTATTACGAAATTAACAACCAGCAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACGGAAACTAGGACTGGACCGCGATATCAtt                                                            >  2:367090/20‑251 (MQ=255)
                                                                                                                                                                        tttGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTg                                                >  1:68006/1‑250 (MQ=255)
                                                                                                                                                                           gCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGAGATATCATTGTTAATAAAGTGaa                                              >  2:472222/1‑249 (MQ=255)
                                                                                                                                                                                ttGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAgcgc                                         >  2:145069/1‑249 (MQ=255)
                                                                                                                                                                                         aaGTTACCGATGTCAACCTCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTTTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTCCACTTTGATCCATACCTGTGCACTTACTATTACGAAATTAACACCCATAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACGAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATg                              <  1:321576/251‑1 (MQ=255)
                                                                                                                                                                                           gTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGCCGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcc                            >  2:225028/1‑251 (MQ=255)
                                                                                                                                                                                                 gATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCt                       >  1:17690/1‑250 (MQ=255)
                                                                                                                                                                                                  aTGTCAACCGCTACCGTCGTGGTGCAATCGCCATGACGTATAACAGCATGCCGCTAGAATTGTTCAAGACGCTGAAAAAAGAGATCACGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGAACTGAAACTAGGTATGGACCGCGATATCATTGTTACTAACGTGAAAGCGAAGGGCAACATGCCCGCCTAt                     >  2:510969/1‑251 (MQ=255)
                                                                                                                                                                                                          cGCTACCGTCGTGGTGAACTCGACCTGACGTATAACAGCATGACGATCGAATTGTTCCAGCAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCAATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGCGAAAGCGCAGGGCAACATGCCCGCCTATGGTAaca              >  2:530471/1‑250 (MQ=255)
                                                                                                                                                                                                                   atgggtgAAATCAAAATGACGTATAACACCAGGCCAATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACAAATTTCCTGTTGTTCCATACCTGTGCCCTTACTATTACGAAATTAAAATCCAAAACCCGCCAGTCAACGATGTGCGTTTGCGTACCGCACGGAAACTAGGTAGGGACCGCGATATCATTGTTAATAAATTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGta    <  1:247872/248‑1 (MQ=255)
                                                                                                                                                                                                                     gggtaaaaTCGACATGACGTATAACAGCAGGCCGATCGAATTGTCCCAAAAGCAGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCTTGTGCACTTCCTATTACGAAATTAACACCAAGAACCCGCCATTCAACAATGGGCGTGTGCGTACCGCACTGAAACTAGGTAGGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGtata  <  2:60706/246‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       
GCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATA  >  NC_000913/1301760‑1302223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: