Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,253,002	C→T	intergenic (+17/+83)	dhaR → / ← ycgV	DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,253,002	0	C	T	100.0%	110.8 / NA	32	intergenic (+17/+83)	dhaR/ycgV	DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/19);  total (13/19)

GAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACTTCTCCCGGG  >  NC_000913/1252761‑1253245                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    gAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATc                                                                                                                                                                                                                                            <  1:91791/251‑1 (MQ=255)              ccGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTa                                                                                                                                                                                                                               <  2:380609/251‑1 (MQ=255)                gAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTAtt                                                                                                                                                                                                                             <  1:212972/251‑1 (MQ=255)                 aGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTAttt                                                                                                                                                                                                                            <  1:417753/251‑1 (MQ=255)                        gACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGAAATTATTAACGCAGCCCAGGTCACAGGCGGGCGCATTCCGGAAATGTCGGCTTTAATTGGGATCGGCCGCACGACCCTGTGGCGGAAAATGAATCCACATGGCATTGA‑GTACTGGCAGTTTAAACGCCGGGGTAT‑AAAGACAGAAACGATTTTTTATAAATCAGAGTGAGTTGTTTTTCATTCCg                                                                                                                                                                                                                     >  1:172483/1‑251 (MQ=255)                                          cACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTTGCATTCAGGAAAAGTCGGCTTTTCTTGGGGTCGGGCGCACTACGCTGTGGCGGGAAATGAAACAACAAGGCATTTGTTCA‑GGGGAGGTTAAGCGCCGGG‑GATGAAAGAAAGAAAAGATTTTTTATACAACAAAGTGATCTTTATTTTATTCCCGCGCCCGCTAAAAAtttt                                                                                                                                                                                                   >  1:430676/1‑251 (MQ=255)                                            cccGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑GATGAAAGACAGAAACGATTTTTGATACATCCGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAAttttt                                                                                                                                                                                                  >  1:446650/1‑249 (MQ=255)                                                          aGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAggg                                                                                                                                                                                   <  2:352066/251‑1 (MQ=255)                                                               gTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCCCAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCTCGCTAACAATTTTCAGCATCGTTTAAGGGCt                                                                                                                                                                                 <  1:2747/248‑1 (MQ=255)                                                                                       ggCAATTATTAACGCAGCCCAGGTCACAGGCGGTTGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAg                                                                                                                                                      <  2:430676/251‑1 (MQ=255)                                                                                                taACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGTCAGTTTAAGCGTCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATGTCATTCCGGCGCACGCTAAGAATTTTCAGCAGCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATAtcc                                                                                                                                              <  1:42042/250‑1 (MQ=255)                                                                                                  aCGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGGAAATGAAGCAAAATGGCATTGATGCA‑AGGCAGTTTAAGCGCCGGG‑GATGAAAGAAAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTTCGCCTCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATAtcctcc                                                                                                                                           >  1:297025/1‑251 (MQ=255)                                                                                                          cAGGTCGCAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCAGTCCGGCGCACGCTAACAAGTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACtt                                                                                                                                   <  1:348382/251‑1 (MQ=255)                                                                                                               cacaGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTc                                                                                                                              <  2:478714/251‑1 (MQ=255)                                                                                                                 caGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTg                                                                                                                            <  2:98649/251‑1 (MQ=255)                                                                                                                 caGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTg                                                                                                                            >  1:191931/1‑251 (MQ=255)                                                                                                                         cGCATTCAGGAAATGTCGGATTTACTTAGGATCGGCCGCACTCCGCTGTGGCGGAAAATGAAGAAACATGGCATTGGTGCA‑GGGCAACTTAAGCGCCGGG‑TATAAAAGACAGCAACGATTTTTGATACATACGAGTGATCTGTATTTCATTCCGGCGCCCGCTCAAAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCCCTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTTATCaa                                                                                                                       >  2:68677/1‑248 (MQ=255)                                                                                                                                       gTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATGTTCAGCATCGTTTAAGGGCTTGGCTATCCCGCACATAAAAGCTGAAGCGATATCCGCCGTTGACTTGCTCCGGATCAAAGAGGTTTCCCTGCgtg                                                                                                      <  2:185676/251‑1 (MQ=255)                                                                                                                                         cGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCgtgt                                                                                                     <  2:191931/250‑1 (MQ=255)                                                                                                                                                aCTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCt                                                                                             >  1:74629/1‑251 (MQ=255)                                                                                                                                                             cGCACTACGTTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTGGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAAtg                                                                                <  1:455469/251‑1 (MQ=255)                                                                                                                                                                        gtgGCGGAAAATGAAGCAACATGGCAATGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCtgttt                                                                      <  2:428421/250‑1 (MQ=255)                                                                                                                                                                               aaaaTGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑CATGAAAGACAGAAACGATTTTTGATACATCAGCGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACt                                                              >  2:209045/1‑251 (MQ=255)                                                                                                                                                                                     aaGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACCGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATAc                                                               >  1:360287/1‑244 (MQ=255)                                                                                                                                                                                     aaGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATAc                                                               <  2:360287/244‑1 (MQ=255)                                                                                                                                                                                                                     aGCGCCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTAcc                         >  2:305340/1‑251 (MQ=255)                                                                                                                                                                                                                        gcCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATc                                                                                                 >  1:70508/1‑176 (MQ=255)                                                                                                                                                                                                                        gcCGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCAGCGTTTAAGGGCTTGTCTATCCCGCACTGAAAAGCTGAATCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATc                                                                                                 <  2:70508/176‑1 (MQ=255)                                                                                                                                                                                                                          cGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAgg                                                                                                                  >  1:275017/1‑157 (MQ=255)                                                                                                                                                                                                                          cGGG‑TATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCGCCGTTGACTTGCTTCTGATCAAAGAgg                                                                                                                  <  2:275017/157‑1 (MQ=255)                                                                                                                                                                                                                                             aCGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACTTCTCCCggg  >  1:12429/1‑251 (MQ=255)                                                                                                                                                                                                                                             aCGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACTTCTCCCggg  <  2:446650/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCA‑GGGCAGTTTAAGCGCCGGG‑TATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACTTCTCCCGGG  >  NC_000913/1252761‑1253245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑