Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,153,007	G→A	A357T (GCG→ACG)	fabF →	3‑oxoacyl‑[acyl carrier protein] synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,153,007	0	G	A	96.2%	86.3 / NA	26	A357T (GCG→ACG)	fabF	3‑oxoacyl‑[acyl carrier protein] synthase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (0/25);  minor base T (0/1);  total (0/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAATGGCGCAGGCGCAGCTCTGGCGATGGCAAATGCTCTGCGTGATGCAGGCATTGAAGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCT  >  NC_000913/1152762‑1153229                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                               aaaaTGGCGCAGGCGCAGCTCTGGCGATGGCAAATGCTCTGCGTGATGCAGGCATTGAAGCGAGTCAGATTGGCTACGTTAACGCGCGCGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTg                                                                                                                                                                                                                           <  1:517134/251‑1 (MQ=255)      ggcgcaggcgcaGCTCTGGCGATGGCAAATGCTCTGCGTGATGCAGGCATTGAAGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGcgcg                                                                                                                                                                                                                       <  1:399038/250‑1 (MQ=255)         gcaggctcagCTCTGTCGATGTCCAAGGCTCTGCGTGATGCAGCCATTGAATCGAGTCAGATTTGCTACGTGAACGCGCACGGGACTTCTACGCCTGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCGTCGGTGAAGCTTCAAGCCGTGTGTTGGGAAGCTCCACGAAAGCTATGACCGGTCACCGGCGAGCTGCTGCGGGTGGATTAGAATCTATCTATTCCATCCGGACGCTGTGGGATCa                                                                                                                                                                                                                   <  2:534651/251‑1 (MQ=255)            ggcgcagCTCTGGCGATGGCAAATGCTCTGCTTGATGCAGGCATGGAAGCGAGTCAGATTGGCGACGTTAACGCGCACGGTACTGCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGATCACCTGTTAGGTGCGTCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGc                                                                                                                                                                                                                <  1:502590/251‑1 (MQ=255)                                                  aGGCATTGAAGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTTCTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAg                                                                                                                                                                          <  1:393782/251‑1 (MQ=255)                                                          aaGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCGACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGGTGGTAAGCGCCACGAAATCTATGACCGGTCACCTGATAGGTGCGGCGGGTGCAGTAGAATCTATCTACGCCATCCTGACGCTGCGCGATCATGCTGTGCCGCCAACCCTCAACCTGGATAACCCGGATGAAGGTTGCGAt                                                                                                                                                                  <  2:348307/251‑1 (MQ=255)                                                                                   cgcgCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAgcgc                                                                                                                                         <  2:150505/251‑1 (MQ=255)                                                                                                   aCGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCGTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGAGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGGTAGCgg                                                                                                                            <  2:357/248‑1 (MQ=255)                                                                                                                            cgcAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttc                                                                                                <  1:278703/251‑1 (MQ=255)                                                                                                                                            gagcTTCGGTGAAGCTGCAAGCCGGGTGTTGGTAAGCTCCACGAAAGCGATGACCGGTCAGCTGTTAGGTGCGGCGGGTGCAGTAGAATCTAGCGACTCCATCCTGACGCTGCGCGAGCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                                                                                <  1:197663/248‑1 (MQ=255)                                                                                                                                                 acggggAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGGGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGGCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTGCGGTGGCACTaa                                                                               <  2:257822/242‑1 (MQ=255)                                                                                                                                                           tGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCAGCAACCGGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                                                                                <  2:288114/236‑1 (MQ=255)                                                                                                                                                             cAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCttt                                                               <  2:429026/251‑1 (MQ=255)                                                                                                                                                              aaGCCGGGTGCTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCGGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTa                                                              <  1:177646/251‑1 (MQ=255)                                                                                                                                                                gCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGGGGCACTAATGGTTCTTTGATCTTTaaa                                                            <  2:504569/251‑1 (MQ=255)                                                                                                                                                                       gtTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATc                                                      <  1:2512/250‑1 (MQ=255)                                                                                                                                                                                  gcgcGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCAGCAGCCTGGATAACCCGGATGAAGGTGTCTAGCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGGGGAATGGAATTCACTCTGTGTAACTCCTTCGGCTGCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCAt                                           <  1:330703/247‑1 (MQ=255)                                                                                                                                                                                  ccACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCAtt                                          <  1:196302/251‑1 (MQ=255)                                                                                                                                                                                  ccACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATGTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCa                                            <  1:235409/249‑1 (MQ=255)                                                                                                                                                                                   cACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCAttt                                         <  2:212868/251‑1 (MQ=255)                                                                                                                                                                                             aTGACCGGTCACCTGGTAGGTGCTGCGGGTGCAGAAGAATCTATCTACTCCATCCTGTCTCGTCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATCACCCGGATGCAGGTTGCGATCTGGAGTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTTGCGCTAATGGTTCTTTTAGCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCt                                 <  1:225208/249‑1 (MQ=255)                                                                                                                                                                                                 ccGGTCACCTGGTATGTGCGGCGGGTGCAGTAGAATCTATCTACGCCATCCTGACGTTGCGCGATCAGGCTGTGCAGCCATCGATCATCCTGGATAACCCGGATGAAGTTTGCGACCTGGATTTCGTACCGCACTAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTGGAACTTTAAAAATATCTAAGTTTTCAGCTTCCAACCGTATaaa                           <  1:418618/251‑1 (MQ=255)                                                                                                                                                                                                             tAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGGCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTg                <  2:553446/250‑1 (MQ=255)                                                                                                                                                                                                              aGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGc               <  1:36365/250‑1 (MQ=255)                                                                                                                                                                                                                        ggTGCAGTAGAATCTATCTACTCCATCCTGACGCTGCTCGATCAGGCTGTTCCGCCACCCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACTCTCTGTGTAACGCCTTCGGCTTCTGTGGCACTAATGGTTCTTTTATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTGAGAAAAGGGCCGCTTGCGGGCCcttttt    <  2:519849/251‑1 (MQ=255)                                                                                                                                                                                                                           tcAGTAGAATCTATCTACTCCATCCTGACGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTTGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGGCATTTTCCACCCTTATAAAAGGTCCGCTTGCTGGCCTTGTTTCt  <  2:314418/249‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                               AAAATGGCGCAGGCGCAGCTCTGGCGATGGCAAATGCTCTGCGTGATGCAGGCATTGAAGCGAGTCAGATTGGCTACGTTAACGCGCACGGTACTTCTACGCCGGCTGGCGATAAAGCTGAAGCGCAGGCGGTGAAAACCATCTTCGGTGAAGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCT  >  NC_000913/1152762‑1153229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑