Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,527	C→T	E405K (GAA→AAA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,527	0	C	T	100.0%	154.1 / NA	47	E405K (GAA→AAA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/27);  total (20/27)

CAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCG  >  NC_000913/822289‑822768                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  cAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTg                                                                                                                                                                                                                                       <  1:313182/251‑1 (MQ=255) cAGGTCAAAGCAGCCGTAAAACAGATAGCGGACGTCCACCAGACCTACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCATACCCCTTCCCAGTCCACTTTGTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAGCAGCCAGGTGAGGTTCTTCTGTGCTAAGCGCCAGCGCGGGTGTCATTTTCTCATCAGGGGTTTACCCCCGTGT‑TTTCGTTTTTTACTCGATCCTg                                                                                                                                                                                                                                       <  2:326185/251‑1 (MQ=255)      cATAGCAGCCGTAAATCAGATATCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAAGCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGtt                                                                                                                                                                                                                                   <  2:392245/250‑1 (MQ=255)               cGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCg                                                                                                                                                                                                                         >  1:37804/1‑251 (MQ=255)                                     caccaGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTg                                                                                                                                                                                                   >  2:520314/1‑251 (MQ=255)                                          gCCCGACGGCACTAAGCAGCGCAGCGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCGCTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGGGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTGCGCATCAGGTTGTTACCCCCGGGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCAGTGTCGGTTGTGCGGGCGGATCAACCTGCGCCa                                                                                                                                                                                              <  1:236816/251‑1 (MQ=255)                                              gACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCATACg                                                                                                                                                                                          >  2:476139/1‑251 (MQ=255)                                                   cACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCACTTCCCAGTCCACTTTTTTGGAGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTGCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGAGCAACCTGCGCCAGACGTGGCg                                                                                                                                                                                     <  2:70579/251‑1 (MQ=255)                                                     cTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGCTCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCgtg                                                                                                                                                                                   >  1:2693/1‑251 (MQ=255)                                                       aaGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCgtgt                                                                                                                                                                                  <  1:123801/250‑1 (MQ=255)                                                              gcAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCggg                                                                                                                                                                          >  1:136988/1‑250 (MQ=255)                                                                              cgcgTATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAgc                                                                                                                                                           <  1:126401/250‑1 (MQ=255)                                                                                cgGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCCGCGcc                                                                                                                                                        >  2:112644/1‑251 (MQ=255)                                                                                   aTGACCTTCCAGACCTCTTCCCAGGCCACTTGTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCggg                                                                                                                                                     <  1:67325/251‑1 (MQ=255)                                                                                          tCCAGACCTCTTCAGAGTACACTTTTTTGGCGTAGACCACCAGCAACACAATCCCCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTTGCGTGTGTGGCGGGAGCCAGCCAACCAGCGCCGGGACGTGGc                                                                                                                                              >  2:79228/1‑251 (MQ=255)                                                                                              gACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTaa                                                                                                                                          <  1:112644/251‑1 (MQ=255)                                                                                              gACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCAAGCCAACCAGCGCCGGGAAGTGGCGTa                                                                                                                                           >  1:329283/1‑250 (MQ=255)                                                                                                  cctTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAg                                                                                                                                      <  2:136988/250‑1 (MQ=255)                                                                                                          gTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGc                                                                                                                              <  1:201860/251‑1 (MQ=255)                                                                                                              ccttgtttGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGc                                                                                                                          <  2:152016/246‑1 (MQ=255)                                                                                                                            gACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCCGCGCCGGGAAGGGGCGTAAAAAGTGGGACGCCAGCACGCTTTTGGTc                                                                                                              >  2:215662/1‑249 (MQ=255)                                                                                                                                     cAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGTGTGTGAGTTACTCAGCAGGGTTTGCCCCTCGTGT‑TTACAGTTTTTACCCGATCCTGCGTTTCCATTGGCGGTTGTGCGGGCGGAGCAACCTGGGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGTCGTAAAAAGTGGAACGCCAGCACGCTTTTGTTCAGGTTccacca                                                                                                   <  1:543184/251‑1 (MQ=255)                                                                                                                                            aTCACCGCGATAAAAAACCGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGCTCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAATCCAGCCAACCACCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCCCGCTTTTGGTCAGGTTCCACCAGGTGCGt                                                                                            >  1:158123/1‑251 (MQ=255)                                                                                                                                                         aaaaCAGCCAGGTGCGGATCTTCTTTGCTAAGCGCCAGCGCGGGAGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGCTCCTGCGTTTCCATTGTCGGATGTGCGGGCGGATCAACCTGCGCCAGCCGTGGCGTGGGGGCCGGAAGCCAGCCCACCAGCGCCGGGAAGTGGCGGAAAAAGTGGAACGCCCGCAAGCTTTTGGTAAGGTTCCACAAGGTGTGGTTGGGCCGCATgg                                                                               >  2:530785/1‑251 (MQ=255)                                                                                                                                                           aaCAGCCATGTGAGGATCTTCTTGGCGAAGCGCCAGCGCGTGTGTGATTTACGCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGGCAGGTTCCACCAGGTGCGGGTGGGCAGCATGGtt                                                                             <  2:500350/251‑1 (MQ=255)                                                                                                                                                                ccAGGTGAGGATCATCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCAGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTTCGGGCGGATCAACCTGCGCCAGACGAGGCGTGTGTGCGCGAAGCCAGCCAACCAGCGCCGGGACGTGGCGTAAAAAGTGGAACGCCAGAACGCTTTGGGTCAGGTTCCACCAGGTGAGTTTGGGCAGCATGGTTTCaac                                                                        >  1:368519/1‑249 (MQ=255)                                                                                                                                                                  aggggAGGATCTTCTTTGCTAAGCGCCGGCGCGGGTGTGGTTTACTCATCAGGGTTTTACCACAGTGT‑TTTCAGTTTTTCCCCGATACTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGCAGTGGCGTGTGTGCCGGAAACCCGCCACCCCGCGCCGGGAAGTGGCGTAACACGTGGCACGCCAGCCCGCTTATTGTCAGGATCCACCAGGGTCGGTTGGGCAGCATGGTTTCAtacc                                                                      >  2:361665/5‑248 (MQ=255)                                                                                                                                                                                 ttGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCt                                                        <  1:321662/250‑1 (MQ=255)                                                                                                                                                                                  tGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCCGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGc                                                      >  1:397813/1‑251 (MQ=255)                                                                                                                                                                                   gCTAAGCGCCAGCGCGGGTGTGATTTACTCATCATGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATGGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGGGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCGCCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCg                                                     <  1:260847/251‑1 (MQ=255)                                                                                                                                                                                             aGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGc                                           <  2:37804/251‑1 (MQ=255)                                                                                                                                                                                              gcgcGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGcc                                          <  1:523594/251‑1 (MQ=255)                                                                                                                                                                                                   ggTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCa                                     <  1:549741/251‑1 (MQ=255)                                                                                                                                                                                                     tgtgATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAg                                    <  2:160218/250‑1 (MQ=255)                                                                                                                                                                                                      gtgATTTACTCATCAGGGTTTTACCCCCG‑GTGTTTCAGTTTTTACCCGTTCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGAt                                  <  2:397813/251‑1 (MQ=255)                                                                                                                                                                                                        gATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTGTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTTGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTGTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGAtt                                 <  2:413415/250‑1 (MQ=255)                                                                                                                                                                                                         aTTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTAt                               <  1:520314/251‑1 (MQ=255)                                                                                                                                                                                                          tttACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATc                              >  1:121166/1‑251 (MQ=255)                                                                                                                                                                                                                catcaGGGTTTTACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCa                         <  1:215662/250‑1 (MQ=255)                                                                                                                                                                                                                      ggTTTTACCCCCGTGT‑TTTCAGTTTTTACCCTATCCTGCGTTTACATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTTTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCAAGCTTTTGGTCAGGTTCCCCCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTTCTGACAATCTCCGGAAATAATGCCGTTCAGATTTTCGCGCAGCGTCTg                  >  1:470261/1‑251 (MQ=255)                                                                                                                                                                                                                        ttttACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCCGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCCTCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGt                >  1:53892/1‑251 (MQ=255)                                                                                                                                                                                                                          ttACCACCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGGAGTGGCGTAAAAAGAGGAACGCCCGCCAGCCTTTGGTCAGGTTCCACCAGGGGCGTTTGGGCCGCATGGTTTTATCCACCTGCTGACAATCTGCGGCAATAATGCCGGTCAGATTATCGCGCAGccgcaggttc              >  2:380366/1‑242 (MQ=255)                                                                                                                                                                                                                           tACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGGGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTa              >  1:553271/1‑250 (MQ=255)                                                                                                                                                                                                                           tACCCCCGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGAGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACAAGCGCCGGGAAGTGGCGGAAAAAGTGGAAAGCCAGAACGATTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGACGTTCAGATTATCGCGCAGCGTCTGGTTa              >  1:185824/1‑250 (MQ=255)                                                                                                                                                                                                                                ccGTGT‑TTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGGGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTGTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGa        <  1:529448/251‑1 (MQ=255)                                                                                                                                                                                                                                    gt‑TTTCAGTTTTTGCCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTTTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGa     <  2:516329/250‑1 (MQ=255)                                                                                                                                                                                                                                        ttCAGTATTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGGGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTACACCAGGTGCGTATGGGCAGCATGGTTTCACCCACCTGCGGACAATCTGCGGCAATAATGCCGTGCAGATTATCGCGCCGCGGCTGGTTAAAATGACGATCg  <  2:226699/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  CAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGT‑TTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCG  >  NC_000913/822289‑822768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑