Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	753,977	G→A	R164R (CGC→CGT)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,977	0	G	A	100.0%	174.2 / NA	50	R164R (CGC→CGT)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/28);  total (22/28)

CCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGGCGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTC  >  NC_000913/753732‑754216                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 cccGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGCTTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGac                                                                                                                                                                                                                                             >  2:386032/1‑250 (MQ=255) cccGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGa                                                                                                                                                                                                                                              >  2:504205/1‑250 (MQ=255)  ccGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAAc                                                                                                                                                                                                                                            <  1:506181/251‑1 (MQ=255)    gAAGAGCCAGCGGTACGCACGGAGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCACGGGAAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CAAAAGCAGACGGAAcgc                                                                                                                                                                                                                                          >  2:293291/1‑251 (MQ=255)           cAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAAtt                                                                                                                                                                                                                                   >  1:367192/1‑251 (MQ=255)                gTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACg                                                                                                                                                                                                                              >  1:329587/1‑251 (MQ=255)                  aCGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGt                                                                                                                                                                                                                            <  1:126552/251‑1 (MQ=255)                                    ggCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACAt                                                                                                                                                                                                          <  2:128439/251‑1 (MQ=255)                                       gTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATcc                                                                                                                                                                                                        >  2:378097/1‑250 (MQ=255)                                                  ggTCAGCGTGCAGAAACAGAAGTCGGTCCTTAGCACGTGCCAGAATCGGATTAACTTCAGACGGTTCGCACGGCTTGGAGACCATCATATTCAGGAAGTAACCGGCGGAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGTCCATG‑TCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTAACGAGGATTGTTAACATCCAGCGAGTCGTGa                                                                                                                                                                                            <  2:225267/250‑1 (MQ=255)                                                                           gTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTaa                                                                                                                                                                   <  1:293291/251‑1 (MQ=255)                                                                             ccATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAAt                                                                                                                                                                  <  1:304921/250‑1 (MQ=255)                                                                                tAGCACGTTCCAGAATAGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACAGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAAAGCGGCAATTTCACGGTGACGCGGATTGTTAACATCCAGCGAGTCGTGATAGACAGCCGCCAGCGAGCCGGTAATaac                                                                                                                                                               >  1:241599/1‑248 (MQ=255)                                                                                         ccAGAATCGGATTAACTGCATACGGTTCGCACGGCGTGGAGAACAGCATATTCAGGAAGTTACCGGCGTAGGAGATATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGGGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGAc                                                                                                                                                      <  2:528542/250‑1 (MQ=255)                                                                                                   aTTAACTGCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGAt                                                                                                                                           <  1:270602/251‑1 (MQ=255)                                                                                                          tCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGt                                                                                                                                     <  1:159026/250‑1 (MQ=255)                                                                                                              aCGGTTCGCACGGCGTGGCGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTcgcg                                                                                                                                 >  1:160185/1‑250 (MQ=255)                                                                                                              aCGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGa                                                                                                                                <  1:536330/251‑1 (MQ=255)                                                                                                              aCGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACGGCCATTGGATGCGAGTcgcg                                                                                                                                 <  2:324928/250‑1 (MQ=255)                                                                                                              aCGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACAGCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGa                                                                                                                                <  1:406828/251‑1 (MQ=255)                                                                                                                ggTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAAAGCGGCAATTTCACGGTGACGAGGATTGTTAACATCAAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACg                                                                                                                              >  2:320921/1‑251 (MQ=255)                                                                                                                 gTTCGCACGGCGTGGAGAACATCATAGTCAGGAAGGTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGGCTTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACAGGACTGCCATCGGATGCGATTCGCGACgg                                                                                                                             <  2:516380/251‑1 (MQ=255)                                                                                                                    cGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGaa                                                                                                                           <  1:281760/250‑1 (MQ=255)                                                                                                                     gCACGGCGTGGAGAACATCATATTCAGGACGTTACCGGCGTGGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTTCGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGGCTGCCATAGGATGCGAGTCGCGACGGaa                                                                                                                           >  2:455854/1‑250 (MQ=255)                                                                                                                       aCGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGc                                                                                                                        >  1:167215/1‑250 (MQ=255)                                                                                                                       aCGGCGTGGAGAACATCATATTCAGGAAGTTAACGGCGTAGGAGAGATCGTTGCGCGGGGAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCCGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGc                                                                                                                        >  2:128904/1‑250 (MQ=255)                                                                                                                                  aaCATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACg                                                                                                           >  1:224195/1‑251 (MQ=255)                                                                                                                                   aCATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACgg                                                                                                          >  2:131838/1‑251 (MQ=255)                                                                                                                                             cAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAAGACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCt                                                                                                <  1:40637/251‑1 (MQ=255)                                                                                                                                              aGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTc                                                                                               <  2:160185/251‑1 (MQ=255)                                                                                                                                                       ccGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCAt                                                                                      <  1:423401/251‑1 (MQ=255)                                                                                                                                                           cGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGt                                                                                   >  1:185689/1‑250 (MQ=255)                                                                                                                                                                    gaTCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTc                                                                         >  1:298715/1‑251 (MQ=255)                                                                                                                                                                            cgcgGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGt                                                                 <  2:541096/251‑1 (MQ=255)                                                                                                                                                                              cgGGTAAACAAATGGCTGACCAATGGCATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGAAGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCAAGAGCGCCGGTAATACCACACATGACTGCCATTGGCTGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATAATGGAATGACGGGTCACCGTAGttt                                                               >  1:106177/1‑251 (MQ=255)                                                                                                                                                                              cgGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGttt                                                               <  1:504205/251‑1 (MQ=255)                                                                                                                                                                               gggTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCTCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGtttt                                                              <  2:308668/251‑1 (MQ=255)                                                                                                                                                                                   aaaCAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTaaa                                                           <  2:2632/250‑1 (MQ=255)                                                                                                                                                                                       aaaTGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGt                                                      <  1:386032/251‑1 (MQ=255)                                                                                                                                                                                       aaaTGGCTGACCAATGGAATACTTGGAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATGGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACTGTAGTTTTAAATTCGt                                                      <  2:70250/251‑1 (MQ=255)                                                                                                                                                                                             cTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGACGTGATAGAACGCCGCCAGCGCGCCGGTAATCCCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACt                                                >  1:355418/1‑251 (MQ=255)                                                                                                                                                                                                           aCTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGt                                  <  1:29419/251‑1 (MQ=255)                                                                                                                                                                                                               gTAACCCATCGCGGCCAGGGGCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCTCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGGTTTAAATTCGTCATACTGTTCCTGAGTCGGtctt                               <  2:485150/250‑4 (MQ=255)                                                                                                                                                                                                               gTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGttttt                              >  2:345206/1‑251 (MQ=255)                                                                                                                                                                                                                   cacatgccgGCCATAGTCGGCACTTT‑CGACAGCAGACGCACCGCGGCAATTTCACGGTGACGCGGATTGTTACCATACAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATAACACACATGCCTGCCATTGGATGCGAGTAGAGACGGACAGCATGGCAAC‑GACGTGTAATCTGCTCGTGGATCATGGAATGACGGGTCACCGTAGTTTTAAATTCGTCCTACTGTTCCTGAGGCGGTTTTTctac                          >  2:481757/8‑248 (MQ=255)                                                                                                                                                                                                                     caTCGCGGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCAt                        >  2:427015/1‑251 (MQ=255)                                                                                                                                                                                                                       gcgcgGCCATGGTCGGCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACTAGGATTGGTAACATCCAGCGAGTCGTGAGAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGTTATGACGGGTCTCCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTc                      <  1:110129/250‑1 (MQ=255)                                                                                                                                                                                                                                     ggCATTTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑a                                                                                                                  >  2:276538/1‑145 (MQ=255)                                                                                                                                                                                                                                     ggCATGTT‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATATCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑a                                                                                                                  <  1:276538/145‑1 (MQ=255)                                                                                                                                                                                                                                            t‑CGACAGCAGACGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTc  <  2:126460/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 CCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTT‑CGACAGCAGGCGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGG‑AACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTC  >  NC_000913/753732‑754216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑