Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	694,941	G→A	L5L (CTC→CTT)	miaB ←	isopentenyl‑adenosine A37 tRNA methylthiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	694,941	0	G	A	100.0%	197.6 / NA	55	L5L (CTC→CTT)	miaB	isopentenyl‑adenosine A37 tRNA methylthiolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/31);  total (24/31)

TGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACA  >  NC_000913/694696‑695177                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             tGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCGGACAGCCCCAGGTTTTAATATGAAGtt                                                                                                                                                                                                                                          <  2:450470/250‑1 (MQ=255)           cGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATc                                                                                                                                                                                                                               <  1:356118/251‑1 (MQ=255)                  cccGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGCCTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGc                                                                                                                                                                                                                        <  1:512773/250‑1 (MQ=255)                   ccGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGct                                                                                                                                                                                                                       >  1:314170/1‑251 (MQ=255)                         ccGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGCGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCg                                                                                                                                                                                                                 >  2:469263/1‑251 (MQ=255)                            aTAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGaa                                                                                                                                                                                                               >  1:249268/1‑250 (MQ=255)                              aaTCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCGCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAAt                                                                                                                                                                                                             <  2:103241/250‑1 (MQ=255)                                  aGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAACtagt                                                                                                                                                                                                          >  1:54312/1‑249 (MQ=255)                                               tctcTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGc                                                                                                                                                                                              <  1:286756/248‑1 (MQ=255)                                                         cAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAgtg                                                                                                                                                                                 <  2:314170/251‑1 (MQ=255)                                                          aGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAgtgt                                                                                                                                                                                >  1:197801/1‑251 (MQ=255)                                                            tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTa                                                                                                                                                                               >  1:509612/1‑250 (MQ=255)                                                                cAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCATGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCTAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCt                                                                                                                                                                          <  2:551275/251‑1 (MQ=255)                                                                        cAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGcc                                                                                                                                                                  <  1:50784/251‑1 (MQ=255)                                                                         aaCTGATAGACGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGACATAGTAGCCAGGAATGCAGGGCGAATAGTGTAATGCTTTGCTGCCg                                                                                                                                                                 >  2:70041/1‑251 (MQ=255)                                                                             gATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCCCATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTtgt                                                                                                                                                             <  1:422636/251‑1 (MQ=255)                                                                             gATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTtgt                                                                                                                                                             >  1:215595/1‑251 (MQ=255)                                                                             gATGGAAGACTTTTTCCGGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCGGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTtgt                                                                                                                                                             <  2:448660/251‑1 (MQ=255)                                                                              aTGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTtgtg                                                                                                                                                            >  1:123133/1‑251 (MQ=255)                                                                               tGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGCAATAGTAGCCAGGAATGCa                                                                                                                                                                                             >  1:148590/1‑217 (MQ=255)                                                                               tGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCACCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCa                                                                                                                                                                                             <  2:148590/217‑1 (MQ=255)                                                                                             cTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGCATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGtt                                                                                                                                             >  2:163256/1‑251 (MQ=255)                                                                                                ggcctttgcgcgGATTGAGGAGGTGGTACGCATCAGCGCGTCCGCTTCTTCCGACACGTCGGTCAGGTGAGAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTTCTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGGCGTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAAAGCTTTGCTGCCGTTGTGACCAGTATGGGCGTTATc                                                                                                                                          <  2:457882/243‑1 (MQ=255)                                                                                                     tctcGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGgc                                                                                                                                                                                         >  2:211260/1‑199 (MQ=255)                                                                                                     tctcGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCGTGCGAAAGAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGcc                                                                                                                                      <  1:546819/250‑1 (MQ=255)                                                                                                     tcgcgcgGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGgc                                                                                                                                                                                         <  1:211260/196‑1 (MQ=255)                                                                                                        cgcgGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTa                                                                                                                                  <  1:235876/251‑1 (MQ=255)                                                                                                         gcgGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGACGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCtt                                                                                                                                   >  1:526572/1‑249 (MQ=255)                                                                                                            gATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAgggg                                                                                                                              >  1:260742/1‑251 (MQ=255)                                                                                                            gATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAgggg                                                                                                                              >  1:33279/1‑251 (MQ=255)                                                                                                                                ggagatctgccGCATCTTCCGCCGCGTCGGTCAGTTGATGTCCGTGGGTGTCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACATCCCCAGGTTTTAATAGGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATTCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTaa                                                                                                          <  2:434261/242‑1 (MQ=255)                                                                                                                                      caTCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCat                                                                                                     >  1:208574/1‑250 (MQ=255)                                                                                                                                           gCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGGGGGTGGCATCCAGCCGATAGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCt                                                                                               >  1:181106/1‑251 (MQ=255)                                                                                                                                              tcttcCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCAGCTGACAGCCCCAGGTTTGAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGTGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGGAAAAATCCTGTAAACTTAAAGGATATTGCTaa                                                                                             <  1:468901/250‑1 (MQ=255)                                                                                                                                                  gcgccaaatCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCAATCTTCGATGAATAGTACTCGTTCCTCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGGAGCCAGAAATGCAGGGCGCATAGTGTAATGCTTTGATGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAgg                                                                                         >  2:364174/9‑250 (MQ=255)                                                                                                                                                   cGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGgat                                                                                       >  1:153673/1‑251 (MQ=255)                                                                                                                                                   cGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGgat                                                                                       >  1:131265/1‑251 (MQ=255)                                                                                                                                                             gTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCt                                                                                               <  1:156647/233‑1 (MQ=255)                                                                                                                                                             gTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCt                                                                                               >  2:156647/1‑233 (MQ=255)                                                                                                                                                                       aGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATca                                                                    <  2:215595/250‑1 (MQ=255)                                                                                                                                                                          cGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAAGGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGGAAAAATCCTGTAAACTTAAATCATATTGCTAACAGGATGATTGACCATGACAAATcaacca                                                                <  2:526572/251‑1 (MQ=255)                                                                                                                                                                                 ggCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGaaa                                                         <  1:51934/251‑1 (MQ=255)                                                                                                                                                                                   cATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAAtt                                                       <  2:539646/251‑1 (MQ=255)                                                                                                                                                                                                    aTCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAACCTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATcaacca                                                                >  2:486478/1‑225 (MQ=255)                                                                                                                                                                                                    aTCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATcaacca                                                                <  1:486478/225‑1 (MQ=255)                                                                                                                                                                                                    aTCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCggagg                                      >  1:119397/1‑251 (MQ=255)                                                                                                                                                                                                           aTGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAAGTGCCATTGTCGGCGGAGGAATGGTc                               <  2:447607/251‑1 (MQ=255)                                                                                                                                                                                                           aTGAATCGTACTCGTTCATCTGACAGCCCCAGGTGTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTc                               <  2:181106/251‑1 (MQ=255)                                                                                                                                                                                                                       cGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATAGTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTg                   <  2:143423/251‑1 (MQ=255)                                                                                                                                                                                                                                gACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCTGCGGCGCACTGGCGCTgggg          <  1:216070/251‑1 (MQ=255)                                                                                                                                                                                                                                gACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTgggg          <  2:474837/251‑1 (MQ=255)                                                                                                                                                                                                                                gACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTgg            <  1:336878/249‑1 (MQ=255)                                                                                                                                                                                                                                 aCAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGc         >  1:408283/1‑251 (MQ=255)                                                                                                                                                                                                                                      cccAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGca    <  2:153673/251‑1 (MQ=255)                                                                                                                                                                                                                                        cAGGTTTTAATATGAAGTTTTTGGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGcaca  <  2:509612/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             TGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACA  >  NC_000913/694696‑695177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑