Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	627,348	G→A	V427M (GTG→ATG)	entE →	2,3‑dihydroxybenzoate‑AMP ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	627,348	0	G	A	100.0%	194.4 / NA	56	V427M (GTG→ATG)	entE	2,3‑dihydroxybenzoate‑AMP ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/22);  total (34/22)

GATGATAGCGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGT  >  NC_000913/627102‑627589                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gatgatAGCGCGGAGAAAATTATCCATCCCCAGGGGTACCCAATGTGGCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACGCCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCTCCATGCa                                                                                                                                                                                                                                               <  2:289176/251‑1 (MQ=255)     atAGCGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTAGGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTAGTGATCCATAGGGTTACATCACCATGCAgggg                                                                                                                                                                                                                                           <  2:30095/251‑1 (MQ=255)       aGCGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGgcg                                                                                                                                                                                                                                         <  1:149737/251‑1 (MQ=255)       aGCGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGgcg                                                                                                                                                                                                                                         >  2:106409/1‑251 (MQ=255)         cgcgGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGcgcg                                                                                                                                                                                                                                       >  1:221718/1‑251 (MQ=255)                           tACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGACGTCGGACGCCTGCTGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGTTCCAGAGGGTTACATCACCATGCAGGGGCGCGCGAAAGATCAGATTAAcc                                                                                                                                                                                                                     >  2:210282/1‑251 (MQ=255)                                    ttACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGAGCAGATTAACCGTggcgg                                                                                                                                                                                                              >  1:67459/1‑249 (MQ=255)                                      acccatagtgtCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTT‑GATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGGTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCgag                                                                                                                                                                                                          <  2:320496/243‑1 (MQ=255)                                      aCCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCTTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATTAGATTAACCGTGGCGGCgag                                                                                                                                                                                                          <  2:97164/251‑1 (MQ=255)                                      aCCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCga                                                                                                                                                                                                           <  1:532712/250‑1 (MQ=255)                                         cAATGTGTCCGGCTGACGAAGTATGGGTTGCCGATGCCGAAGGACATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTACGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCAACATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCgaga                                                                                                                                                                                                         >  1:350549/1‑249 (MQ=255)                                               gtCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCt                                                                                                                                                                                                 >  1:299244/1‑251 (MQ=255)                                                  cGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGcc                                                                                                                                                                                              <  1:450647/251‑1 (MQ=255)                                                    gATGACGAAGTATGGGTTGCCGATGCCGAAGCAAATCCCCTGCCGCAAGGGGAAGTCGGACCCCTGATGACGCGAGGGTCGCACACCTTCCGCGGCTATTACAAAAGTCCACAGAACACTGCCGGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATATGATCTCTCTTGTTCAAGCGGGTCACATCACCATGCAGGGCAGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGAGCGCTGCCg                                                                                                                                                                                             >  2:491974/1‑250 (MQ=255)                                                      ggccGAAGTATGGGGTGCCGATGCCGAAGGAAATCCACTGACGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTGCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTAGTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGAGTAACCGTGGCGGCGAGAAGATCGCTGCCGAag                                                                                                                                                                                          <  2:7308/248‑1 (MQ=255)                                                               aTGGGTTTCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGGCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGATATTACAAAAGGCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGGTTTTACTGCTCCGGCGATCTGATCTCTATTGATCCAGAGGGGTACATCATCATGCAGGGGCGCGAGAAAGACCAGAGTAACCGTGGCCTCGAGAAGATCGCGGCCGAAGAGATCGaa                                                                                                                                                                                  <  2:316782/250‑1 (MQ=255)                                                                 gggTTGCCGATGCCGAAGGAAATCCACGGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGaaaa                                                                                                                                                                                <  2:386400/250‑1 (MQ=255)                                                                  ggTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAAcc                                                                                                                                                                              >  1:228773/1‑251 (MQ=255)                                                                    ttGCCGATGCCGAAGGAAATCAACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTACGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACctg                                                                                                                                                                            >  1:379595/1‑251 (MQ=255)                                                                      cccgatgccgaAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCGGATGACGCGCGGGCCGGACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGAGCTCTACTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAATAGATCGAAAACctgct                                                                                                                                                                          <  2:350549/250‑1 (MQ=255)                                                                        cgatgccgaAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACACTGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACctgctgc                                                                                                                                                                        >  1:223693/1‑251 (MQ=255)                                                                         gatgccgaAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCGGATGACGCTCGGGCCGTACACCTGCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCGTTGATTCCAACTGTTTTTTCTGTCCCGGCGATCTGATCTCTAGTGATCCAGAGGTTTACATCTCCAGGCAGGGGCGCGAGAAAGATGAGTTTAGCCGTGGCGGCGAGAAGATCGTTGCCGAAGAGTTCGAAACCctgctgct                                                                                                                                                                       <  2:214746/251‑1 (MQ=255)                                                                                          aCTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGa                                                                                                                                                      <  1:496227/251‑1 (MQ=255)                                                                                                                     gatgaCGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGa                                                                                                                             >  2:15213/1‑249 (MQ=255)                                                                                                                      atgaCGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCCCTGGTGAGCATGGa                                                                                                                             >  2:304638/1‑248 (MQ=255)                                                                                                                            cgcgGGCCGTACACCATCCGCGGCTATTACAAAAGTCCACAGCCCAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCt                                                                                                                    >  1:430378/1‑251 (MQ=255)                                                                                                                              cgGGCCGTACAACTTCCGCGGCTATAACAAAAGTCAACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGCTCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTg                                                                                                                   >  2:273510/1‑250 (MQ=255)                                                                                                                               ggtccGTCCACCTTCCGCGGCTATTACAAAAGTCCACAGCCCACTGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTCTTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGACGATCGCTGCCGAAGAGATCGACAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCCCGCTGGTGAGCATGGAAAATGATCTGAt                                                                                                                 >  2:553708/4‑251 (MQ=255)                                                                                                                                ggCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGCGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTggtg                                                                                                                >  1:307780/1‑248 (MQ=255)                                                                                                                                ggCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGACTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATg                                                                                                                >  1:369664/1‑251 (MQ=255)                                                                                                                                  ccGTCCACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGCGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCCTGGAAGATGAGCTGATggg                                                                                                              >  2:425089/1‑251 (MQ=255)                                                                                                                                     tACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGAATGGTAGATGAGCTGATggg                                                                                                              >  2:333604/1‑248 (MQ=255)                                                                                                                                       cacCTTCCGCGGCTATTACAAAAGTCGACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTGCCGGCGATCTGATCGCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGAGCGAAAACCTGCTGCGGCGCCACCCTGCGGTGATCTACGCTGCACTGGTGAGCATGGAAGATGAGCTGAGGGGCGaaa                                                                                                         <  2:261039/251‑1 (MQ=255)                                                                                                                                          cTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGaaaaa                                                                                                       >  1:272006/1‑250 (MQ=255)                                                                                                                                                        aCAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCtg                                                                                        >  2:361950/1‑251 (MQ=255)                                                                                                                                                           aaaGTCCACAGCACAATGACAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGAAGGGGCGAGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGGGCTGATGGGCGAAAAAAGCGGCGCTTATCtgggg                                                                                     >  2:469948/1‑249 (MQ=255)                                                                                                                                                                       acaATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGc                                                                                                                                  >  1:510282/1‑194 (MQ=255)                                                                                                                                                                       acaATGCCAGCGCCTGTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGc                                                                                                                                  <  2:510282/194‑1 (MQ=255)                                                                                                                                                                       acaATGCCAGCGCCTGTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGAGCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTTGTGAGCATGGAAGATGATCTGATGGGCGAAAAAAGCTGCGCTTAGCTGGTGGTAAAAGAGCCg                                                                         <  2:80352/251‑1 (MQ=255)                                                                                                                                                                         aaTGCCAGCGCCTTGGAGGCCAACGGTTTTAACTGTTCCGGAAATAAGATCTCTATTGATCAAGAGGGTTACATCACCATGCAGGGGCGAAAGAAAGATCAGATTAACCGTAGCGACGAGAAGATCGATGCCGACGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGAGAGCTCCGACGCAATGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCt                                                                       >  2:488329/1‑251 (MQ=255)                                                                                                                                                                            gCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCGGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCAGGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTgc                                                                     <  2:72820/250‑1 (MQ=255)                                                                                                                                                                              cAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTgcgcg                                                                  >  1:137478/1‑251 (MQ=255)                                                                                                                                                                                     tttGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGCGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGc                                                            >  2:78099/1‑250 (MQ=255)                                                                                                                                                                                        gATGCCAACTGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTTCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTGATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAgg                                                         <  2:379595/250‑1 (MQ=255)                                                                                                                                                                                        gATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAgg                                                         <  1:361950/250‑1 (MQ=255)                                                                                                                                                                                         aTGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTg                                                       >  1:394529/1‑251 (MQ=255)                                                                                                                                                                                         aTGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATGAACCGGGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCGTATCTGGTGGGAAAAGAGCCGCTGCGCGCGGTGCAGGTg                                                       <  2:400958/251‑1 (MQ=255)                                                                                                                                                                                                 ggTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGttt                                               >  2:397089/1‑251 (MQ=255)                                                                                                                                                                                                    ttttACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCt                                            >  1:536760/1‑251 (MQ=255)                                                                                                                                                                                                           gTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGGGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGa                                      >  1:26673/1‑250 (MQ=255)                                                                                                                                                                                                              ccGGCGATCTGATCACTATTGATCCAGAGGGTTACATCGTCATGCAGGGGCGCGATAAAGATCAGATTAACCGTTGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCTCCCGGCTGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACa                                   <  1:229810/250‑1 (MQ=255)                                                                                                                                                                                                                              tATTGATCCAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAAttt                   >  2:172160/1‑250 (MQ=255)                                                                                                                                                                                                                                     ccAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTAcc           >  1:417696/1‑251 (MQ=255)                                                                                                                                                                                                                                      cAGAGGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGGGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGGATTGCCGAATTTaagttcccg          >  1:178254/1‑244 (MQ=255)                                                                                                                                                                                                                                       agagGGTTACATCACCATGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCgg         <  1:210282/251‑1 (MQ=255)                                                                                                                                                                                                                                              tACATCACCATGCAGGGGCGCGAGAAAGATCAGCTTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGt  >  1:429968/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GATGATAGCGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGATGACGAAGTATGGGTTGCCGATGCCGAAGGAAATCCACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGT  >  NC_000913/627102‑627589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑