Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	81,102	G→A	S286L (TCG→TTG)	leuB ←	3‑isopropylmalate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	81,102	0	G	A	100.0%	208.3 / NA	68	S286L (TCG→TTG)	leuB	3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/29);  total (39/29)

CATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGC  >  NC_000913/80862‑81344                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   cATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGGTTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGccgcccgc                                                                                                                                                                                                                                          >  1:376584/1‑251 (MQ=255)       tACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTc                                                                                                                                                                                                                                    >  1:323194/1‑251 (MQ=255)         cagcccTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGACGGCAGCGCCACGGGCTAAATCCCCGGTGCGCATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCCATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCCGGGCAAGGATTTGTGCACT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCAAAGCCGCACGCCGGTTCAt                                                                                                                                                                                                                                  >  2:177653/4‑251 (MQ=255)                   gCTACATAGCGGGCAATGTTATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCCCGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCa                                                                                                                                                                                                                          <  1:62986/249‑1 (MQ=255)                   gCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTACCGGCGGCAGCGCCCCGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAAGGGCGCAAGCCGCATCATCGGCAGCCAGGCTGTAACGCAG‑CAGCAGGGCCAGCGAAAGGTTTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCaaa                                                                                                                                                                                                                        <  2:541956/251‑1 (MQ=255)                       cATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCt                                                                                                                                                                                                                    >  1:52868/1‑251 (MQ=255)                          aGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGCTGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCCAAACCTTGc                                                                                                                                                                                                                 >  1:208380/1‑251 (MQ=255)                            cGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGCATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCACAACCTTGCTc                                                                                                                                                                                                               >  1:213424/1‑251 (MQ=255)                              ggCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAAACTTGCTCGt                                                                                                                                                                                                             >  1:466269/1‑251 (MQ=255)                                  aTGATATCGCCCATTTCCTCGGTACTACCGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAg                                                                                                                                                                                                         <  1:1808/251‑1 (MQ=255)                                  aTGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCCAAACCTTGCTCGTTc                                                                                                                                                                                                           >  1:203178/1‑249 (MQ=255)                                    gATATCGCCCATTTCATCGGTACTACCGGCGGCAGCGCCACGGGCTAAATCCCCGTTGCGAATGCCTTCTTCTAATGCGCGGTTACTGG‑CGCGGTCAATGGCGCAAGCCGCATCCTCGGCATCCAAGCTGTAACGCAG‑CAGCCGTGCCAGCGACAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGGTCATACAGTCCCAAACCTTGCTCGttaaagc                                                                                                                                                                                                       >  2:467292/1‑246 (MQ=255)                                     atatCGCCCATTTCATCGTTACAAACGGCGGCAGCGCCACGGGCTAAATCCCCGGGGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGGGCCGGCGAAAGGATTTGTGCAAT‑CGGGTGGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTGCATACAGTCCAAAACCTTGCTCGTTCAGGCt                                                                                                                                                                                                      <  1:202127/251‑1 (MQ=255)                                         cGCCCATTTCAGCGGTACTAACGCGGGCAGCTCCACGGGTTAATCCCCCGGTGCGAATGCTTTCTACTAATGCGCGGTTATGGG‑CGCGTTCAATGCCGCAAGCCGCTTCACCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCATG‑CGGGTTGCCGAGGTTGTTGCCTGCGAGATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCg                                                                                                                                                                                                  <  1:285675/251‑1 (MQ=255)                                                ttcggcGGTCCTAACGGCGGCATCGCCATGGTCTAAATCCCCGGTGCAAATGCCTTCTTCTAATTCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGTCTGTAACCCAG‑CAGCAGTGCCAGCAAAAGGATGTGTGCATT‑TGGTTTGGCGTTGTTTTTGCCTGCTATATCTGGTCCCAAGCCTCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGc                                                                                                                                                                                            <  1:88814/245‑1 (MQ=255)                                                ttCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTCCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGAAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGc                                                                                                                                                                                            <  1:87377/250‑1 (MQ=255)                                                        tACTAAGGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGATGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGATTTAGCCTGCGAAATCAGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCCGAACCTTGCTCGTTCAAGCTGGCGGGCGGCCACATcccc                                                                                                                                                                                   >  2:89142/1‑251 (MQ=255)                                                               ggcggcAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCCTACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGCAGGCAACATCCCCATCGAGc                                                                                                                                                                            >  2:161304/1‑251 (MQ=255)                                                               ggcggcAGCGCCACGGGCTAAATCCCCGGTGAAAATGCCTTCTTCTCATGCGCGGTTAATGG‑CGCGTTCAATGGCGACCGCCGAATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGc                                                                                                                                                                            >  1:475304/1‑251 (MQ=255)                                                                  ggcAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGcc                                                                                                                                                                           >  1:79499/1‑249 (MQ=255)                                                                  ggcAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGcc                                                                                                                                                                           >  1:141044/1‑249 (MQ=255)                                                                           aCGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAgg                                                                                                                                                                                                        >  1:390935/1‑211 (MQ=255)                                                                              ggCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCCTCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGGTGGCGATGTTTTTGCCCGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAAACTTGCTCGTTCCGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTgcgc                                                                                                                                                             >  2:208727/1‑251 (MQ=255)                                                                                cTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCAc                                                                                                                                                           >  2:325348/1‑251 (MQ=255)                                                                                  aaaccccgggTGCGAATGCCTTCTTCTAAGGGGCGGTTAAGGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCCTCCAGGCTGAAACGCAG‑CAGCAGTGCCAGCGAAAGGTTTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTc                                                                                                                                                         <  2:136214/243‑1 (MQ=255)                                                                                    aTCCCCGGTGCGAATGCCTTCTTCTAATGCGCGTTTAATGG‑CGCGTTCAAGGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGGTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGt                                                                                                                                                       <  1:551869/251‑1 (MQ=255)                                                                                            ggggattgcttgtttctAAGGCGCGGT‑AATGGGCGCGTTCAAGGGGGAAAGCAGAATCAACGGCACCCAGGATGCAGCG‑AGTCAGTAGTGCCAGCGAAGGGATT‑GTGGAATTCGTGTGGGCTATTTTTTGGCCTGCTATATCGGGGGCCAGGCCGCCCGCCGGTTCATACAGGCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCAATGCGCACTCGTcagacaga                                                                                                                                               <  2:240381/238‑1 (MQ=255)                                                                                               gAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGGTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGCAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATg                                                                                                                                            >  2:43304/1‑251 (MQ=255)                                                                                               aaaTGCCTTCTTCTAAGGGGCGTTTAAGGG‑CGCGTTCAATGGCGCAAGCCGATTCATCGGCACCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGTATTTGTGCAAT‑CGGGTTGGCGAGGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATg                                                                                                                                            <  2:237474/250‑1 (MQ=255)                                                                                                aaTGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGt                                                                                                                                           >  2:304096/1‑251 (MQ=255)                                                                                                   gCCTTCTTCTAATGCGCGGTTAATGG‑CAAGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGCTGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAAACTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGCGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGc                                                                                                                                        >  2:541370/1‑251 (MQ=255)                                                                                                       tcttctAAGGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCa                                                                                                                                      <  1:45922/249‑1 (MQ=255)                                                                                                                         aTGG‑CCCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCGGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCAcagcag                                                                                                                  <  1:292788/251‑1 (MQ=255)                                                                                                                          tGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGAACGAATGTCGCCAAACAGGTTGGAGCAcagc                                                                                                                    >  2:132547/1‑248 (MQ=255)                                                                                                                                 gTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTc                                                                                                            >  1:93584/1‑250 (MQ=255)                                                                                                                                 gTTCAATGGCGCAAGACGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACgacc                                                                                                           >  2:389701/1‑248 (MQ=255)                                                                                                                                   aaaaTGGCCCAAGCCGCAGCCTCGGCATCCGGGCTTTAACGCAG‑CAGCGGTCCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTCCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCaaa                                                                                                         <  2:323194/249‑1 (MQ=255)                                                                                                                                        ggCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgtg                                                                                                    >  2:274722/1‑251 (MQ=255)                                                                                                                                         gcgcAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCAAGCAAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTGGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGa                                                                                                   <  2:96989/251‑1 (MQ=255)                                                                                                                                          cgcAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGCAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTACAAAACCTTGCTCGTTAAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCCGAACGTCAAACTGTGAt                                                                                                  >  1:283424/1‑251 (MQ=255)                                                                                                                                          cgcAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGAt                                                                                                  >  2:13422/1‑251 (MQ=255)                                                                                                                                           gcAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATg                                                                                                 >  1:146091/1‑251 (MQ=255)                                                                                                                                           gcAAGCCGCATCATCGGCATCCAAGCTGTAACGCAG‑AAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAAACTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGCCAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgtgctg                                                                                                 >  1:146068/1‑248 (MQ=255)                                                                                                                                                      catcGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCCAT‑CGGGATGGCGCTGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTAATACAGTCCAAACCCTTGCTCGGTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGAACTCGTCAGACAGAATGTCGCCAAACAGGTTGGCGCACAGCAGAACGTCAAACTGTGATGGATCTTTactc                                                                                      >  2:461302/1‑248 (MQ=255)                                                                                                                                                         cGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGATGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACCGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGc                                                                                   >  2:545770/1‑251 (MQ=255)                                                                                                                                                          ggCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCt                                                                                  >  1:292112/1‑251 (MQ=255)                                                                                                                                                          ggCATCCAGGCTGTAACGAAG‑CAGCAGTGCCAGCGAAAGGATTTGGGCAAT‑CGGGTTGGCGATGTTTTTGCCGGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCt                                                                                  <  2:500432/251‑1 (MQ=255)                                                                                                                                                            cATCCAGGCGGTAACGCAG‑CAGCAGTGCCAGCGAAAGGTTTTGGGCAAT‑CGGGTTGGCGATGTTTGTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAAGCAGCTGc                                                                                <  2:79499/251‑1 (MQ=255)                                                                                                                                                                     tGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                                                       >  1:130036/1‑251 (MQ=255)                                                                                                                                                                      gcatcGCAG‑CAGCAGTTCGAGCGAAGGGATGTGTGCAAT‑CGTGTTGGCGATGTTTTTGCCTGCGATATCGGGTTCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCGGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTTCGCACTCGTCAGAGAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGc                                                                        <  1:65785/245‑1 (MQ=255)                                                                                                                                                                      gTAACGCAG‑CAGAAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGt                                                                      <  1:304096/251‑1 (MQ=255)                                                                                                                                                                        accGCAG‑CGGCATTTCCAGCGAAGGGATTGGTGCAAT‑GTGGTTGGCGATGTTTTTGCCGGCTATATGTGGTGCCACGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGATCCAGTGATCATTGCGCACTCGTCAGACATAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGCATCTTTAATCAGCTGCATTGTGGCGTTg                                                                    <  2:258908/249‑1 (MQ=255)                                                                                                                                                                           cgctgcacctgTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTTGCGATTTTTTGGCCTGCGATATCTGGCCCCACGCCCCCCGCCTGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGGGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTc                                                                  <  1:249725/241‑1 (MQ=255)                                                                                                                                                                                cagcagCGCCAGCGACAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATAAAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTAGATGt                                                             >  2:366150/1‑251 (MQ=255)                                                                                                                                                                                   cagTGCTAGCGAAAGGATTTTTGCAAG‑CGGGTTGGCGAGTTTTTTGCCGGCGATACCTGGTCCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGGTGTCGATGTACa                                                          <  2:376584/251‑1 (MQ=255)                                                                                                                                                                                        ccatcGAAAGGTTTTTTGCAAT‑CGGGTTGGCCATGTTATTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATgc                                                     <  1:261885/247‑1 (MQ=255)                                                                                                                                                                                          aGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACTGTCCAAAACCTTGCTCGATCAGGCTGGCGGACGGCCACATCCCCATCGAGCCAGTGCTCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCGGACCGTCAAACTGTGATGGCTCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATacgc                                                   >  2:248914/1‑251 (MQ=255)                                                                                                                                                                                            agAAAGGATTGGTGCAAT‑GGGGTGGGGGATGTTTTGGCCTGAGATATCGGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCAGCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGAGCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCa                                                 <  2:101169/250‑1 (MQ=255)                                                                                                                                                                                               aaGGTTTTGTGCAAT‑CGGGTGGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGtt                                              <  1:441120/251‑1 (MQ=255)                                                                                                                                                                                                  gATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGGCAAGCCGCCCGCCGGTTCATACAGTCCAACACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTAGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGCTCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCgc                                           >  2:26224/1‑250 (MQ=255)                                                                                                                                                                                                   aTTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGTTGTACATATGCGCCAGTTCGAc                                          >  2:165054/1‑251 (MQ=255)                                                                                                                                                                                                               cGGGTTGGCGATTTTTTTGCCGGCGTTATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGGCGCCAAACAGGTGGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTAtt                               <  2:269338/251‑1 (MQ=255)                                                                                                                                                                                                                gggTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTc                              >  2:407672/1‑251 (MQ=255)                                                                                                                                                                                                                    gggCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGTTATTCCGTg                          <  1:192624/250‑1 (MQ=255)                                                                                                                                                                                                                             ttttGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATACGCCAGTTCGACATCCGGGCATTCAGTGGCGGTCTCg                 >  1:514373/1‑251 (MQ=255)                                                                                                                                                                                                                                     ccGATTTCTGGTGCCACGCCGCCCGCCGGTTCATACAGTCCAAAACCGTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGa          <  2:504927/249‑1 (MQ=255)                                                                                                                                                                                                                                          atCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTccc    >  1:466331/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggTGCCAAGCCCCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGc  <  1:525866/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   CATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGG‑CGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAG‑CAGCAGTGCCAGCGAAAGGATTTGTGCAAT‑CGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGC  >  NC_000913/80862‑81344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑