Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,742	G→A	G276D (GGT→GAT)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,742	0	G	A	100.0%	216.6 / NA	60	G276D (GGT→GAT)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/28);  total (32/28)

GAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGT  >  NC_000913/534495‑534980                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                gAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGGTTGatat                                                                                                                                                                                                                                              >  1:94800/1‑251 (MQ=255)  aaaaaGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGCGTTTGATATc                                                                                                                                                                                                                                             >  1:28417/1‑251 (MQ=255)       gCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTa                                                                                                                                                                                                                                         >  1:178331/1‑250 (MQ=255)          gTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAAcc                                                                                                                                                                                                                                      <  1:193385/250‑1 (MQ=255)               aaTGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGCTCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTg                                                                                                                                                                                                                                >  1:248098/1‑251 (MQ=255)                aTGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGc                                                                                                                                                                                                                               <  2:519509/251‑1 (MQ=255)                     aaTCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAAc                                                                                                                                                                                                                           <  1:414159/250‑1 (MQ=255)                     aaTCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACCGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAAcc                                                                                                                                                                                                                          >  1:434786/1‑251 (MQ=255)                      aTCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCg                                                                                                                                                                                                                         >  2:401200/1‑251 (MQ=255)                             ccGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCCGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TAcc                                                                                                                                                                                                                 >  2:288937/1‑251 (MQ=255)                               gAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCgg                                                                                                                                                                                                               >  1:119846/1‑251 (MQ=255)                               gAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGCGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCgg                                                                                                                                                                                                               >  2:54099/1‑251 (MQ=255)                                     ccGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTTACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATAGCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGt                                                                                                                                                                                                         <  1:332891/251‑1 (MQ=255)                                      cGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGTGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTa                                                                                                                                                                                                        <  1:404763/251‑1 (MQ=255)                                        gTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGGTGCAGCTGCCATGTTACAACAGTTGGCTGAACTTACCATCTTCCCGGAGATCCCAAAGCTATTGGGTTGGGGCTGTATCCCTGAGGATCAGGAACTGATGGCCGGGATGGTGGGTCTGCAAACGGCGTTTCGTTACGTTAACGCTCCGTTGCTTGTGGCTGACATGGTGTTGGATATCGGAAACCGTTTCGCTATCTGTGA‑TACCGGCTCGGTagg                                                                                                                                                                                                      <  2:61873/251‑2 (MQ=255)                                        gTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGCCGCAGCTGCACGGTTACAACAGTTTGCTGAACTGACCAGCGTCCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTAGCCCGGCCGATCATGAACGGATGTCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCGGGCGTCTGACATGGTGTTTGATATCGGTAACCGTGTTGCTAACCGTTA‑TACCGGCTTGGTaga                                                                                                                                                                                                      <  2:338870/251‑1 (MQ=255)                                                  ccGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACAcc                                                                                                                                                                                            <  2:404373/251‑1 (MQ=255)                                                   cGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCg                                                                                                                                                                                           >  2:272617/1‑251 (MQ=255)                                                                 ttaatGCTGACGCAGCCGCAATGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCACAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTCACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGAAACCGTGTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATc                                                                                                                                                                             >  2:108139/1‑251 (MQ=255)                                                                  taatGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGTGCTTGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCTTTACGGTAACGCAACGCTGCTGGCGGCTGACATGGTGTTGGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATTCACCGAAGGGCGCAAAATc                                                                                                                                                                             <  2:538589/250‑1 (MQ=255)                                                                    atGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGAGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATCGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAAAGAAATACACCGACGGGCGCAAAATCGtt                                                                                                                                                                          >  1:237059/1‑251 (MQ=255)                                                                            gCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatattg                                                                                                                                                                   >  1:485988/1‑250 (MQ=255)                                                                                   cACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAg                                                                                                                                                           <  1:431597/251‑1 (MQ=255)                                                                                    aCTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGCAGGGCGCAAAATCGTTCATATTGATATTGAGc                                                                                                                                                          >  2:201349/1‑251 (MQ=255)                                                                                        ttACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTACTGGGCTGGGGCTGTATCCCGGACGATCATCAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGCAACGCAACGCTGCTGGCGTCTACCATGGTGTTTGATATCGGTAACCGATTTGCTAACCGTAA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAAACGCTCATATTGCTATTGAGCCGAc                                                                                                                                                      >  1:526742/1‑251 (MQ=255)                                                                                                gttcgcttagctgacgagcgggccGGTGATCCCAACGCTAATGGGCTGGGGCGGTCTCCCTGACGATCATTAACTGATGGCCGGGATGGTGTGTCTGCAAGCCGCGCATCGTTCCGGTAACGCATGGCTGCTGGCTTCTGACATGGTTGTTGATATCGGTAACCGTTTTGCTAACCGTCAATAC‑GGCTCGGTAGAGAAATACAACGAAGGGCGCAAAACCGCTCAGATTGAAACTGAGCGGACGCTAATTg                                                                                                                                              <  2:71458/229‑1 (MQ=255)                                                                                                gTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTTATATTGAGCCGACGCAAATTg                                                                                                                                              >  2:187843/1‑251 (MQ=255)                                                                                                gTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTg                                                                                                                                              <  2:418211/251‑1 (MQ=255)                                                                                                         ccTGACCAGCGTTCCGGGGAGCCCAACGCTAGTGGGCTGGGGCGGTATCCCTGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAGCCGTTTTGCTAACCGTCA‑TACCGGCGCGGTATAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGc                                                                                                                                     <  2:213492/250‑1 (MQ=255)                                                                                                            gACCCGCGTTCCGGTGATCCCAACGGTAATGGGCGGGGGCTGTATCCCGGTCGAGCATGAACTGATGGCCGGGAGGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCtgt                                                                                                                                  <  2:23209/251‑1 (MQ=255)                                                                                                                  cGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGCGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGCAGGGCGCAAAATCGTTCATATTGATATTGAGCAGACGCAAATTGGTCGCGTGCTGTGTCCgg                                                                                                                            >  2:286583/1‑251 (MQ=255)                                                                                                                       cGGTGATCCCAACGCGAATGGGCTGGGGCGGTATCCCGGACGATCATGAACTGATTGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACTCAACGCTGCTGTCGGCTGACATTGTGTGTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCGCGGTAGAGAAATACACCGAATGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGAtctc                                                                                                                       <  1:511166/251‑1 (MQ=255)                                                                                                                         gtgatcacacggCTAATGGGCTGGGGCTGTATCCCGGACGCTCATGAACTGATGGCCGGGCTGGTGGGTCTGCAAACCGCGCATCGTTAAGGTAACGCAACGCTGCTGGCGTCTGAAATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACAACGAAGGGCGCAAAATCGTTCATATTGTTATTGAGCCGACGCACATTGGTCGCGTGCTGTGTCCGGATCTCgg                                                                                                                     >  2:262551/12‑251 (MQ=255)                                                                                                                              cccAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATAAAACGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTg                                                                                                                >  2:440009/1‑251 (MQ=255)                                                                                                                                     ctactGGGCTAGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCCCATAGTTACGGTAACGCCACGCTGCTGCCGTCTGACATGGTGTTTGATCTCGGTAACCGTTTTGATAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAGGGGCGCAAACTCGTTCATATTGATATTGACCCGAAGCAACTTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTg                                                                                                          >  2:256529/5‑250 (MQ=255)                                                                                                                                       aaTGGGCTGGGTCTGTATCCCGGACGATCATGAACTGATTGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGTCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCTGTATTGTCTCTGATg                                                                                                       <  1:79986/251‑1 (MQ=255)                                                                                                                                             ctggggctgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGCAGGGCGCAAAATCGTTCATCTTGATATTGAGCCGACGCAAATTTGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTaa                                                                                                   >  2:389986/1‑249 (MQ=255)                                                                                                                                                    tgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCt                                                                                          >  1:39328/1‑251 (MQ=255)                                                                                                                                                    tgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCt                                                                                          >  1:47099/1‑251 (MQ=255)                                                                                                                                                     gTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTg                                                                                         >  1:172141/1‑251 (MQ=255)                                                                                                                                                         cccGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGacac                                                                                     >  2:479103/1‑251 (MQ=255)                                                                                                                                                          ccGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACAct                                                                                    >  2:516663/1‑251 (MQ=255)                                                                                                                                                              aCGATCATGAACTGATGGCCGGGATGATGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACActgctg                                                                                >  1:25822/1‑251 (MQ=255)                                                                                                                                                                  tCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTg                                                                            >  1:497732/1‑251 (MQ=255)                                                                                                                                                                   cATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTg                                                                            <  1:201349/250‑1 (MQ=255)                                                                                                                                                                                     aTGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCGGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCa                                                         <  2:13971/251‑1 (MQ=255)                                                                                                                                                                                                gCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGgt                                               <  1:158610/250‑1 (MQ=255)                                                                                                                                                                                                gCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGgt                                               <  1:479103/250‑1 (MQ=255)                                                                                                                                                                                                gCAAACCGCGCAGCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCTGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCgg                                                 <  1:24847/248‑1 (MQ=255)                                                                                                                                                                                                    aCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCt                                          <  1:537298/251‑1 (MQ=255)                                                                                                                                                                                                      cgcgCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGc                                        <  1:253146/251‑1 (MQ=255)                                                                                                                                                                                                          cATCGTTACGGTAACGCAACGCTGCTGGCGTCGGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCgtg                                    <  2:445003/251‑1 (MQ=255)                                                                                                                                                                                                                tACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCAtattgatattga                                                                                                                                                            <  2:418171/125‑1 (MQ=255)                                                                                                                                                                                                                tACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCAtattgatattga                                                                                                                                                            >  1:418171/1‑125 (MQ=255)                                                                                                                                                                                                                      aacgcaacgcTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATg                        >  1:393966/1‑251 (MQ=255)                                                                                                                                                                                                                      aacgcaacgcTGCTGGCGTCTGACATGGGGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCCAAATCGGTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGCATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAACGAATg                        >  1:529306/1‑251 (MQ=255)                                                                                                                                                                                                                           aacgcTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCg                   <  1:265843/251‑1 (MQ=255)                                                                                                                                                                                                                            acgcTGCTGGCGTCTGACATTGTGTTTGATATCGGTAACCGTGTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCTTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGGGTCCGGAtctc                                                                                                                       <  2:76882/150‑1 (MQ=255)                                                                                                                                                                                                                            acgcTGCTGGCGTCTGACATGGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGAtctc                                                                                                                       >  1:76882/1‑150 (MQ=255)                                                                                                                                                                                                                                           tccagggTGTTTGATATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTTCTGGTTGAAGTGGCGCAGGAGATGCAAAAATCGGGGCGTCTGCCGTGTCGGATAGAAGGGTTCGCCGACTGCCagcagc    <  2:345988/245‑1 (MQ=255)                                                                                                                                                                                                                                            aCATGGTGTTTGATATAGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGGTCATATTGATATTGAGCCGACGCAAATTGGGCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGACTGCCGTGTCGTAAAGAATGGGTCGCCGGCGGCCCGCAGCGt  >  2:174545/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                GAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCA‑TACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGT  >  NC_000913/534495‑534980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑