Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,622	C→T	T168I (ACC→ATC)	htpG →	chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,622	0	C	T	97.9%	153.9 / NA	48	T168I (ACC→ATC)	htpG	chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (27/19);  minor base G (0/1);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCACCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGAT  >  NC_000913/495381‑495848                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    gacgaAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAaaga                                                                                                                                                                                                                               >  1:310879/1‑248 (MQ=255)        tGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGt                                                                                                                                                                                                                     >  1:193011/1‑251 (MQ=255)          aTTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTAc                                                                                                                                                                                                                   >  2:341757/1‑251 (MQ=255)            tGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATAAACCGTTGCCGACCTCATCAAAGAAGATCGTGGTACTg                                                                                                                                                                                                                 >  2:97796/1‑251 (MQ=255)                cATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAAt                                                                                                                                                                                                             >  1:238112/1‑251 (MQ=255)                            aTCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCAACCTGCATCt                                                                                                                                                                                                 >  2:172805/1‑251 (MQ=255)                               gCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCCAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCg                                                                                                                                                                                              >  1:393566/1‑251 (MQ=255)                                     tCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGAATCTGCGTGAAgg                                                                                                                                                                                        >  1:531009/1‑251 (MQ=255)                                        ggTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCg                                                                                                                                                                                      >  1:472792/1‑250 (MQ=255)                                         gcaCCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGaa                                                                                                                                                                                    <  1:220415/249‑1 (MQ=255)                                              aaaTCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGa                                                                                                                                                                               <  1:24063/251‑1 (MQ=255)                                               aaTCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGGTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAg                                                                                                                                                                              <  2:128730/251‑1 (MQ=255)                                                 gcATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAAGCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑tt                                                                                                                                                                           <  2:321853/250‑1 (MQ=255)                                                    ttCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑tttct                                                                                                                                                                        >  1:305777/1‑248 (MQ=255)                                                         cGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGa                                                                                                                                                                     <  2:117948/249‑1 (MQ=255)                                                                         gACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTc                                                                                                                                                   <  2:348516/251‑1 (MQ=255)                                                                                         gccagcTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATAc                                                                                                                                   >  1:400199/1‑251 (MQ=255)                                                                                                 aTCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGCAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTTCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGAcc                                                                                                                            >  1:386054/1‑250 (MQ=255)                                                                                                   cGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGCCATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGCG‑TTTCTCGATGACTGGCGCGTGCCTTCCATCATCAGCAAATACTCCGACCat                                                                                                                          >  2:75297/1‑250 (MQ=255)                                                                                                   cGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCat                                                                                                                          >  1:160417/1‑250 (MQ=255)                                                                                                    ggTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCatat                                                                                                                        >  1:232510/1‑251 (MQ=255)                                                                                                              gtgtTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCg                                                                                                              <  2:472792/251‑1 (MQ=255)                                                                                                                gtTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGt                                                                                                            <  1:266958/251‑1 (MQ=255)                                                                                                                 ttGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGt                                                                                                            >  1:358597/1‑250 (MQ=255)                                                                                                                  tGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTa                                                                                                           <  1:172700/250‑1 (MQ=255)                                                                                                                    gTTTCTCCTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTagag                                                                                                        <  2:155225/251‑1 (MQ=255)                                                                                                                       tCTACTCTGCGTTTATCGTGTCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCAGCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACGGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCTGTAGAGAt                                                                                                      <  2:353706/250‑1 (MQ=255)                                                                                                                       tCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATTGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGAt                                                                                                      <  2:128238/250‑1 (MQ=255)                                                                                                                          aCTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATc                                                                                                     <  1:131681/248‑1 (MQ=255)                                                                                                                          aCTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGa                                                                                                   >  1:338977/1‑250 (MQ=255)                                                                                                                                        gTGGCCGACAACGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAgaga                                                                                     >  1:134449/1‑250 (MQ=255)                                                                                                                                           gCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGTAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGAcc                                                                                                                            <  1:104946/208‑1 (MQ=255)                                                                                                                                             cGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGCGAATACACAGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACg                                                                               >  1:475499/1‑251 (MQ=255)                                                                                                                                                                           aGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATc                                                  >  2:533187/1‑250 (MQ=255)                                                                                                                                                                              cGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCCTCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATcaac                                               >  1:57131/1‑250 (MQ=255)                                                                                                                                                                               gAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGGGAATACACCGTTGCCGGCATCATCAAAGAAGATCGTGGTACTGAAATCGCCCTGCATCTGCGTGAAGGCGAATACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCTCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATcaacaa                                             <  1:494545/251‑1 (MQ=255)                                                                                                                                                                                 aaaaCCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGGGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAg                                           <  2:528400/251‑1 (MQ=255)                                                                                                                                                                                            aTGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCtg                                >  2:413072/1‑251 (MQ=255)                                                                                                                                                                                              ggCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCtgt                               <  1:172805/250‑1 (MQ=255)                                                                                                                                                                                                cgtcgtCTGGGAATCGGCTGGCGAAGGTGATTACACCGTTGCCGACATCAGCAAAGAAGATCGTGTTACTGAAATCACCCTGCGTCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCAGCATCAGCAAATACTCCGACCATATCGCTCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAATCCGTTATCTCCTGGGAGAAAATCAACAAAGCGCATGCGCTGTGGa                            <  1:271564/251‑1 (MQ=255)                                                                                                                                                                                                  tcttctGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACt                          >  2:447758/1‑251 (MQ=255)                                                                                                                                                                                                     tctGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTc                         <  2:305777/249‑1 (MQ=255)                                                                                                                                                                                                        gggAATCGGCTGGGGAAAGGGAATACACCGGTTCCGACATCAACAAAGAAGATCGTGGTCATGAAAACACCCTTAAGCTGCGTGAAGGCGAAGACGAGGTTCC‑CGATGACTGGCGCGTGCGGTCCATTATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGt                       <  2:415414/248‑1 (MQ=255)                                                                                                                                                                                                        gggAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGAACATATCGCGCTGCCGGTAGAGATCGAAAAACGCGATGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCCGGAGCTGTGGACTCGTAAc                    >  2:113398/1‑251 (MQ=255)                                                                                                                                                                                                                      gAAGGTGAATACACCGTTGCCGACATCATCAAAGAAAATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAAAGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGCGAAAATCAACAAAGAGCAGGCGCTGGGGACTCGTAAAAAGTCGGCAATccc      >  1:295905/1‑249 (MQ=255)                                                                                                                                                                                                                      gAAGGGGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGACAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCAc      >  1:110161/1‑251 (MQ=255)                                                                                                                                                                                                                        aGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCg    >  2:244599/1‑251 (MQ=255)                                                                                                                                                                                                                          gTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCCCCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTTCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGAt  <  2:444470/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    GACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCACCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAG‑TTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGAT  >  NC_000913/495381‑495848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑