Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	471,709	G→A	K358K (AAG→AAA)	mdlB →	ABC transporter family protein MdlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	471,709	0	G	A	100.0%	235.7 / NA	71	K358K (AAG→AAA)	mdlB	ABC transporter family protein MdlB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/33);  total (38/33)

ACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGG  >  NC_000913/471467‑471952                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aCCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTaa                                                                                                                                                                                                                                             <  1:271032/251‑1 (MQ=255)    atggAAGTGGGTGTGCTGTATGCGGTTATCAGCTAGCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGTCTATGCTGCAACAGGCTGTTGTTGCTGGTGAGCTCTTTTTTGAACTGATGGACGGACCGCGCCAGCAAGATTGCAATGATGATCGGCTGTTACAGAGTGTCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAAtct                                                                                                                                                                                                                                          <  1:327284/248‑1 (MQ=255)         aGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCtg                                                                                                                                                                                                                                     <  2:231753/251‑1 (MQ=255)          gTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGGGCTAAAAAACATTAATCTCTCtgt                                                                                                                                                                                                                                    >  1:292116/1‑251 (MQ=255)               cGTGCTGTATGCGTTTATCAGCTATCTTGGCCGGCTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGGGTGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCACTGATGATCGCCCGTTACAGTGTGGCACCATCGAAGTCTATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCta                                                                                                                                                                                                                               <  2:277310/251‑2 (MQ=255)                   cTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATctctct                                                                                                                                                                                                                                      <  1:22968/240‑1 (MQ=255)                   cTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATctctct                                                                                                                                                                                                                                      >  2:22968/1‑240 (MQ=255)                   cTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTcgcg                                                                                                                                                                                                                           >  1:140248/1‑251 (MQ=255)                    tGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAAACATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGCTGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCgcgc                                                                                                                                                                                                                          >  2:110163/1‑251 (MQ=255)                           gTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGCCAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTtg                                                                                                                                                                                                                   >  2:282907/1‑251 (MQ=255)                               aTCAGCTATCTTGGGCGACTTAACGAACCATTACTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCGCCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGgc                                                                                                                                                                                                               >  1:553458/1‑251 (MQ=255)                                tCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGATGCAACAGGCTGTTGTTGCTGGTGAGCGCGAGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGATTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGgcg                                                                                                                                                                                                              >  2:507984/1‑251 (MQ=255)                                    cTATCTTGGGCGCCTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGATTGAACTGATGGACGGACCGCGCCAGCAATATGGCACTGATGATCGCCCGTTACAGAGTTGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACACTCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTgg                                                                                                                                                                                                          >  2:137403/1‑251 (MQ=255)                                      aTCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTgg                                                                                                                                                                                                          <  2:398532/249‑1 (MQ=255)                                      aTCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTc                                                                                                                                                                                                        >  1:117599/1‑251 (MQ=255)                                        cTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCgg                                                                                                                                                                                                      >  1:152939/1‑251 (MQ=255)                                                       gAACCATTAATCGAACTGATAACGCAAAAGGCGGTGATGTAACATGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGAGGCACCATCGAAGTCGATAAAGTGTCATTTGCTTATCGCGATGACAATCAGGTGCTAAAAAACATTAATCTCTCTGTGCATTCGCGCAATTTTGTGGAGCTGGTCGGGCATACAGGCAGTgg                                                                                                                                                                                       >  2:394973/1‑251 (MQ=255)                                                       gAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTgg                                                                                                                                                                                       <  1:538475/251‑1 (MQ=255)                                                         aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCa                                                                                                                                                                                     <  1:379952/251‑1 (MQ=255)                                                                 tCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAcc                                                                                                                                                                             >  1:159507/1‑251 (MQ=255)                                                                  cGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAccc                                                                                                                                                                            >  1:111213/1‑251 (MQ=255)                                                                  cGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAcc                                                                                                                                                                             <  2:177204/250‑1 (MQ=255)                                                                                aaCAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTg                                                                                                                                                              <  2:400625/251‑1 (MQ=255)                                                                                  cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCCATATGGCACTGATGATCGCCCGTTACAGCGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAt                                                                                                                                                            >  1:366883/1‑251 (MQ=255)                                                                                  cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACCGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAt                                                                                                                                                            >  2:536292/1‑251 (MQ=255)                                                                                   aGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATg                                                                                                                                                           <  2:200858/251‑1 (MQ=255)                                                                                       gATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGGGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCt                                                                                                                                                       >  2:472086/1‑251 (MQ=255)                                                                                              cAACAGGCTGTTGTTGCTGGTGAGCGCGTGGTTGAACTGATGGACGGATCGCGTCAGCAATATGGCAATGATGATCGCCCGTTACAGATTGGCACCATCGAAGTCTATAACGTGTCATTTGCTTATCGCGATGAGAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCGTCGCGCAATTTTGTGGCTCAGGGCGGGCATACCGTCAGTGGCGAAAGCACCCTCGCCAGTTTATTGATGGGGTAGTAccc                                                                                                                                                <  2:524075/251‑1 (MQ=255)                                                                                                  atgCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCTCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCCGTGGCACAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCt                                                                                                                                             >  2:298974/3‑250 (MQ=255)                                                                                                   ggCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTa                                                                                                                                            >  2:34906/1‑250 (MQ=255)                                                                                                         tgttgCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGaa                                                                                                                                      <  1:479105/250‑1 (MQ=255)                                                                                                                ggtggtcgcgTGTTTGAACTGTTGGACGGTCCGCTCTGGTAATATGGCAATGATGATCTCCCGTTACAGAGTTGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATATGGTGCTAAAAACCATTAATCTCTCTGGGCCTTCGCGCAATTTTGTGGCTCTGTTCGTGCATACCGGCAGTGGCAAAAGCACCCGCGCCAGAGTATTGCTGGGTTATTAccgcctatcgttagctgag                                                                                                                                <  2:61645/243‑18 (MQ=255)                                                                                                                ggTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTg                                                                                                                                  >  1:383430/1‑247 (MQ=255)                                                                                                                 gTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGTCAACATCTAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACTGAAGGTGAGAtt                                                                                                                             <  2:126986/251‑1 (MQ=255)                                                                                                                   gAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTc                                                                                                                            <  1:473681/250‑1 (MQ=255)                                                                                                                      cgcgTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCt                                                                                                                        <  1:265966/251‑1 (MQ=255)                                                                                                                                        gacggacCGCGACAGCAATATGGAAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTCCCCGCTAACGGCAGGTGAGATTCGCCTTGATGGTCGTCCATTa                                                                                                        >  2:495422/1‑249 (MQ=255)                                                                                                                                           ggacCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATCTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTc                                                                                                   >  1:388109/1‑251 (MQ=255)                                                                                                                                                 cgcCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTACATTAAGTTCGCTa                                                                                               >  2:517404/1‑249 (MQ=255)                                                                                                                                                   ccAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCTCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTc                                                                                           <  1:388507/251‑1 (MQ=255)                                                                                                                                                    cAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTc                                                                                           >  1:315459/1‑250 (MQ=255)                                                                                                                                                      gCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAGGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTcaca                                                                                        <  2:143798/251‑1 (MQ=255)                                                                                                                                                       cAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTcac                                                                                         >  2:105052/1‑249 (MQ=255)                                                                                                                                                        aaTATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGGTTATTGAGGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAgc                                                                                      <  1:366291/251‑1 (MQ=255)                                                                                                                                                         atatGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATATGGTGCTAAAAAACCTTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGTTGGTCGCGCATACCGGCAGTGGCAAAAGCAACCTCGCCAGTTTATTGATGGGCTATTACCCGCTAAAGGACGGTGAGATTCGCCTTGATGGTCGTCCAGTAATTTCGCTCAGtaaaagcg                                                                                     >  2:414422/1‑244 (MQ=255)                                                                                                                                                          tatGGCAATGATGCTCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAgcgc                                                                                    >  2:431471/1‑251 (MQ=255)                                                                                                                                                            tGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAgcgcgc                                                                                  >  1:74481/1‑251 (MQ=255)                                                                                                                                                                   gatgatCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGCTGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGccc                                                                           >  1:376172/1‑250 (MQ=255)                                                                                                                                                                              gTTACAGAGGGGCACCATCGAAGACGATCACGTGTCATTTGCTTATCGCGATGACAACCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAA‑GCACCCTTGCCCGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTAGCATTGATTGTCGTCCCTTAAGGTCGCTAAGTTAACGCGCGCTGCGCCAAGGCGTGGcga                                                                >  2:404560/1‑248 (MQ=255)                                                                                                                                                                                       tGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCGGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTgcagc                                                       >  2:15163/1‑251 (MQ=255)                                                                                                                                                                                         gCACCATCGAAGTCAATAACGTGTCATTTGCTTATCGCGATGGCAATCTGGTGATAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCACACGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTgcagca                                                      >  2:80004/1‑250 (MQ=255)                                                                                                                                                                                            ccATCGAAGTCGCCAACGTCTCATTTGCTTATCGCGATGACCATCTGGTGCTAAAAAACATTAATCTCTCTGTGCATTCGCGCAATTTTGTGGCGCTGGTCGGGCCTACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGCAGGCGAGATTCGCCTTGATGGTCGTCCATTACGTTCGCTAAGCCACAGCGCGCTGCGCCAGGGCGTGGCAATGGGgcagca                                                      >  2:321024/1‑247 (MQ=255)                                                                                                                                                                                             cagcGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCATAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATc                                                 <  1:453853/248‑1 (MQ=255)                                                                                                                                                                                              aTCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATcc                                                <  2:12994/251‑1 (MQ=255)                                                                                                                                                                                                 gAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGAGAGATTCGCCTTGATGGTCGTCCATTACGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCggg                                             >  1:143450/1‑250 (MQ=255)                                                                                                                                                                                                             gtgtCATTTGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGGGAGATTCGCCTTGATGGTCGTCCATTAATTTCGCTAAGTCACAGCGCGCTGCGCCAGGGAGTGGCAATGATGCAGCAAGATCCGGTGGTGCTGGCgg                                  >  2:137211/1‑250 (MQ=255)                                                                                                                                                                                                                     tGCTTATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCGCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTGCGCTAAGGCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAAATCCGGGGGTGCTGGCGGATACCTTc                          <  2:228100/250‑1 (MQ=255)                                                                                                                                                                                                                         tATCGCGATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCGCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCg                      <  2:315459/250‑1 (MQ=255)                                                                                                                                                                                                                         tATCGCGATGACAATCTGGTGCTAAAAAACAGTAATCTCTCTGTGCCTTCGCGCAATTTTTTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCATGGCGTGGCAATGGTGCAGCAAGATCCGGTGTTGCTGGCGGATACCTTCCTc                       <  2:553458/249‑1 (MQ=255)                                                                                                                                                                                                                         aatggcggtgataagcTGGTGCTAAAAACCATGACTCCCTGTGGGCCTTCGCGCAATTTTGAGGCTCTGGTCGGGTAGACCTGCAGTGTCAAAAGCGCTCGCGCCATTTTATTGATGGGCGAGTACCCTCTAACGGAAGGTGAGATTCGCCGTGATGGTCGTCCATTAAGTTCGCTAAGTCACGGCGCGCTGCGCAAGGGCGTGGCAATGGTGCAGCAAGATTCGTTGGTGCTTGCGGATACCTTCCTc                       <  2:5131/234‑1 (MQ=255)                                                                                                                                                                                                                             gcgATGACAATCTGTTGCTAAAAAACACTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGTCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAAc                 <  2:278929/251‑1 (MQ=255)                                                                                                                                                                                                                             gcgATGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAAc                 <  1:472086/251‑1 (MQ=255)                                                                                                                                                                                                                                aTGACAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTg              <  1:517404/251‑1 (MQ=255)                                                                                                                                                                                                                                   aCAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATTCCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACg           <  2:63056/251‑1 (MQ=255)                                                                                                                                                                                                                                   aCAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACg           <  1:15163/251‑1 (MQ=255)                                                                                                                                                                                                                                    cAATCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGc          >  1:325680/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaTCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCt         <  2:140248/251‑1 (MQ=255)                                                                                                                                                                                                                                     aaTCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCt         >  1:132000/1‑251 (MQ=255)                                                                                                                                                                                                                                       tCTGGTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTgg       >  1:210207/1‑251 (MQ=255)                                                                                                                                                                                                                                          ggTGCTAAAAAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGc    >  1:298894/1‑251 (MQ=255)                                                                                                                                                                                                                                             gCTAAAAACCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGTTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGACTTGATGGTCGTCTATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCgg  <  2:160901/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGG  >  NC_000913/471467‑471952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑