Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,479,944	G→A	G72E (GGG→GAG)	yjgN →	inner membrane protein YjgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,479,944	0	G	A	100.0%	58.1 / NA	18	G72E (GGG→GAG)	yjgN	inner membrane protein YjgN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

ATATCAATGGATTTTTTAATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATT  >  NC_000913/4479697‑4480138                                                                                                                                                                                                                                                        |                                                                                                                                                                                                   atatCAATGGATTTTTTAATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGaa                                                                                                                                                                                                 <  1:44616/251‑1 (MQ=255)       aTGGATTTTTTAATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGtttt                                                                                                                                                                                           >  2:524135/1‑251 (MQ=255)                  aaTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATTTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTGAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTc                                                                                                                                                                                <  2:155107/251‑1 (MQ=255)                  aaTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTc                                                                                                                                                                                >  1:216701/1‑251 (MQ=255)                   aTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTAGGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCt                                                                                                                                                                               <  1:551795/251‑1 (MQ=255)                                                 aaTGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCtt                                                                                                                                                 >  1:438118/1‑251 (MQ=255)                                                             gTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAgtgt                                                                                                                                      <  2:216701/250‑1 (MQ=255)                                                                          cGTTTGTTTTTCAGGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAAGGTTTTGGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTGGCAATCTTAATGACAGTGTCAGCAGATATGcc                                                                                                                         <  1:204210/250‑1 (MQ=255)                                                                                                         ttttCTTATTTGTGTGGTGACTGTGTTGTTAACGATTATACCGCTAGGTATCTATTTACCATGGGCATTAATGATATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTTTTTCGCAATCTTAATGACAGTGTCATCAGATATGCCGCTTGTTGTTTGTGTTTTGACTTTGTTACTGt                                                                                         >  2:23503/1‑251 (MQ=255)                                                                                                               tATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGttt                                                                                   >  1:148502/1‑251 (MQ=255)                                                                                                                                       aaCGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCaaa                                                             >  1:207917/1‑249 (MQ=255)                                                                                                                                                                ttACCATGGGCATTAATGAAATGTAAGAGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCtt                                                                                                                     >  1:295341/1‑168 (MQ=255)                                                                                                                                                                ttACCATGGGCAGTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTGTTTCTATTTCGCAATCTTAATGACAGTGTCAGCATATATGCCGCtt                                                                                                                     <  2:295341/168‑1 (MQ=255)                                                                                                                                                                                  aaaTGTAAGCGATATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTATATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCATTAATGACCAGTCTCAACGGCGTa                  >  2:387212/1‑249 (MQ=255)                                                                                                                                                                                    aTGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAAt                                                                                                                                              >  2:65455/1‑123 (MQ=255)                                                                                                                                                                                    aTGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAAt                                                                                                                                              <  1:65455/123‑1 (MQ=255)                                                                                                                                                                                                   ttATGCTAATATGTAAGTTAACTGTCAACGTTTTTCTTATTGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCTCTTGTTGGTTGTGATTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAAt   <  2:148502/247‑1 (MQ=255)                                                                                                                                                                                                   ttATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAAtt  <  2:173732/248‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                   ATATCAATGGATTTTTTAATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATT  >  NC_000913/4479697‑4480138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑