BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,316,498	G→A	H241Y (CAC→TAC)	phnM ←	RPnTP hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,316,498	0	G	A	100.0%	448.5 / NA	135	H241Y (CAC→TAC)	phnM	RPnTP hydrolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (87/48); total (87/48)

Only 100 of 136 total aligned reads displayed.

GTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGCGACACC  >  NC_000913/4316251‑4316742

      cGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGGAGTAGTCGGAAGAGAGGATCTCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGGAATTTGGCGATCACGCTGCCAAGCTGGTAAGATTCAGc                                                                                                                                                                                                                                             >  1:519985/1‑251 (MQ=255)

        gTTGCTCGGGTCATCGGCGACGCGAAAGGCCGCATCGAGGAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCaa                                                                                                                                                                                                                                           <  2:126173/251‑1 (MQ=255)

          tGCTCTGGACATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAAc                                                                                                                                                                                                                                          <  2:140151/250‑1 (MQ=255)

                         cGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGGAGTCGGAAGCGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCAGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGTGCGTGGCTTCCGCCGGTTCGAACGTGGGGGGGAATTCGCCGCTCACGCGGCCGAGCTGGGAAGATTCAGCAAcgtgggcgtgggggggg                                                                                                                                                                                                                          >  1:510544/1‑246 (MQ=255)

                          gACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGt                                                                                                                                                                                                                         >  1:121860/1‑251 (MQ=255)

                             gcgAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGGAAGATTCAGCAACGTGGGCGTGGGTGGCGTCAt                                                                                                                                                                                                                      >  2:132571/1‑251 (MQ=255)

                              cgAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGATTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATc                                                                                                                                                                                                                     >  1:549901/1‑251 (MQ=255)

                              cgAAATGCCGCATCGAGCAGGCTGGCGGGGTAGGAGTCGGAAGAGAGGAAATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCTCAATATTCTGCGCGCCCATCACCACGTTCATGCCATGCTTGCGCGGGGCTTCCGCCGCTTCGACCGCGGGGTGAAATTAGGCGATCACGCTACCAAGCTGGCACGATTCCGCAACGTGGGCGGGGGGGGCggact                                                                                                                                                                                                                      >  2:297923/1‑246 (MQ=255)

                                aaaTGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATACAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGCGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAAATTCAGCAACGTGGGCGTGGGTGGCGTCATCGt                                                                                                                                                                                                                   >  1:49992/1‑251 (MQ=255)

                                  aTGCCGCATCGAGCAAGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGGGCGAGCCGCCGCGCCCAATATTCGGCGTGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGGAATTCGGCGATCACGCTGCCCAGCTGGTAAGATTTAGCAACGTGGGCGTGGGCGGCGTCATCGtgg                                                                                                                                                                                                                 >  2:34150/1‑251 (MQ=255)

                                   tGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCCCGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGtggc                                                                                                                                                                                                                >  1:458600/1‑251 (MQ=255)

                                     ccGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGtggctg                                                                                                                                                                                                              <  1:305262/251‑1 (MQ=255)

                                             aGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGAGTGGGTGGCGTCATCGTGGCTGGCAAgcgc                                                                                                                                                                                                      <  1:151574/251‑1 (MQ=255)

                                                aGGCTGGCGGGGTAGGAGTCGGGAGAGAGGATATCCAGCAGGCCACGCTGCGCCAGTTCACTGGCCGCAACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCCTCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGGTCACGCTGCCAAGCTGGTAGGATTCAGCCACGTGGGCGTGGGGGGCGTCGTCGTGGCTGGCAAGCGCAAt                                                                                                                                                                                                   >  2:134054/1‑251 (MQ=255)

                                                    tGGCGGGGTAGGAGACGGCAGAGAGGCTATCCAGCAGGCCAAGCTCCGCCAGTTCACTGGCCGACACGTTGCCGGAGTGCGCGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGCGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGCGGGGGGCGTCATCGTGGCTGGCAAGCGCAAttttt                                                                                                                                                                                               >  2:356010/1‑251 (MQ=255)

                                                     ggCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTc                                                                                                                                                                                              >  1:99679/1‑251 (MQ=255)

                                                           gtagtaGTCGGAAGAGCGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTcgcgcgg                                                                                                                                                                                        >  1:442467/1‑250 (MQ=255)

                                                              gtagtCGGGAGAAAGGAGCTCCAGCAGGCCAAGCTGCGCCCGCTCGCTGGCCGCCACGTTCCCGGAGTGCGAGCCGCCGCGCACAATATTAGGCGCGCCCATCCGCACGTTCATGCCATGCTTGCGCGAGGCTCCCGCCGCTTCGTACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTACGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGTGGc                                                                                                                                                                                     >  2:101536/1‑251 (MQ=255)

                                                                  gcGGAAGAGAGGATATCCAGCAGTCCAAGCGTCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATGCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAg                                                                                                                                                                                 <  2:210559/250‑1 (MQ=255)

                                                                   cGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAgg                                                                                                                                                                                >  1:188946/1‑251 (MQ=255)

                                                                        gagagGATATCCCGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATCTTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGACCGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGGGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGggcggc                                                                                                                                                                           >  2:267825/1‑251 (MQ=255)

                                                                        gagagGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGggcggc                                                                                                                                                                           >  2:250028/1‑251 (MQ=255)

                                                                         agagGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGgcggcg                                                                                                                                                                          >  1:294070/1‑251 (MQ=255)

                                                                           agGATATCCAGCAGGCCAACCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGCGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGACATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCACCGTGGGCGTGGGTGGCGTCATCGTGGCTGGCACGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGAt                                                                                                                                                                        >  2:58229/1‑251 (MQ=255)

                                                                               tatCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGAt                                                                                                                                                                    >  1:394758/1‑251 (MQ=255)

                                                                                atCCAGCAAGCCAAGCTGCGCCAGTTCACTGGACGCCACGTCGCCGGACTGAGAGCAGCCGCGCACAATCTTCGGCGCGCACATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGAGGGGGGAAATTCGGCGATCACGCTGCCAAGCTGGTACGATTCAGCAACGTGGGCGTGGGTGGCGTCTTCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACCGGGCGGCGCTGGAtt                                                                                                                                                                   >  2:270069/1‑251 (MQ=255)

                                                                                   cagcagGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTcgc                                                                                                                                                                <  2:244966/251‑1 (MQ=255)

                                                                                      cagGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGAt                                                                                                                                                             >  1:107880/1‑251 (MQ=255)

                                                                                      cagGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCGTCGAACGTGGTGGGAAATTCGGCGCTCACCCTGCCAAGCTGCTAAGATTCAGCAACGTGGGCGGGGGTGGCGTCATCGTGGCTGGCAAGACCAATTTTTCGCCCGCGGCACAGGGAGGCGTTGGATACGCGAt                                                                                                                                                             >  2:36349/1‑251 (MQ=255)

                                                                                       agGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATGCGCGAtt                                                                                                                                                            >  1:378422/1‑251 (MQ=255)

                                                                                       agGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGAGGGCGTGGGTGGCGTCCTCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATggcatcgggatt                                                                                                                                                            >  2:62842/1‑241 (MQ=255)

                                                                                          ccAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACCATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGTACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGCGGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGgccgcgtcggggtcgcgattccg                                                                                                                                                         >  2:7698/1‑234 (MQ=255)

                                                                                          ccAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCgg                                                                                                                                                         >  1:252246/1‑251 (MQ=255)

                                                                                                   gcCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGca                                                                                                                                                                                    >  2:191458/1‑215 (MQ=255)

                                                                                                   gcCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGca                                                                                                                                                                                    <  1:191458/215‑1 (MQ=255)

                                                                                                      aGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCg                                                                                                                                             >  2:336534/1‑251 (MQ=255)

                                                                                                          cACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTgc                                                                                                                                          <  1:398351/250‑1 (MQ=255)

                                                                                                              ggCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCCAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCCGCGGgcggcg                                                                                                                                      >  2:468525/1‑250 (MQ=255)

                                                                                                                ccgccACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAgc                                                                                                                                   <  1:320457/251‑1 (MQ=255)

                                                                                                                ccgccACGTTGCCGGAGGGCGAGCCGCCGCGCACAATATTCGGCGCGCCCAGCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAgc                                                                                                                                   <  2:317779/251‑1 (MQ=255)

                                                                                                                    cACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGTTGCGACCAGCGTGCGGCGAGCGcc                                                                                                                                >  2:409308/1‑250 (MQ=255)

                                                                                                                    cACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGATGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAACGTGCGGCGAGCGcc                                                                                                                                >  1:22543/1‑250 (MQ=255)

                                                                                                                          gCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGct                                                                                                                         >  2:35446/1‑251 (MQ=255)

                                                                                                                          gCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGct                                                                                                                         <  1:399235/251‑1 (MQ=255)

                                                                                                                           ccGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGctc                                                                                                                        <  2:86238/251‑1 (MQ=255)

                                                                                                                            cGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGCCCAGCGTGCGGCGGGGGCCAGTTGctct                                                                                                                       >  2:351272/1‑251 (MQ=255)

                                                                                                                            cGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGAGCGGCGAGCGCCAGTTGctct                                                                                                                       >  1:404923/1‑251 (MQ=255)

                                                                                                                             ggAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCtctt                                                                                                                      <  1:250028/251‑1 (MQ=255)

                                                                                                                               aGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCtcttct                                                                                                                    >  2:157652/1‑251 (MQ=255)

                                                                                                                                gTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTcttctt                                                                                                                   >  2:480995/1‑251 (MQ=255)

                                                                                                                                gTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTcttctt                                                                                                                   <  1:69656/251‑1 (MQ=255)

                                                                                                                                  gCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCCACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCg                                                                                                                 >  1:287242/1‑251 (MQ=255)

                                                                                                                                  gCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCg                                                                                                                 <  1:484965/251‑1 (MQ=255)

                                                                                                                                     ggccgccGCGCACAAAATGCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTGCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTTCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTAc                                                                                                              <  2:549901/250‑1 (MQ=255)

                                                                                                                                       ccgccgCGCACAATAGTCGGCGCGCCCAGCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATGCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTTCGGCGAGCGCCAGTTGCTCTTCTTCGTActg                                                                                                            <  2:318523/251‑1 (MQ=255)

                                                                                                                                        cgccgcGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAGATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGCTTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTActgc                                                                                                           >  1:41882/1‑251 (MQ=255)

                                                                                                                                          ccgcGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCTGCGAGCGCCAGGTGCTCTTCTTCGTActgctg                                                                                                         <  1:173106/251‑1 (MQ=255)

                                                                                                                                               cacaATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCt                                                                                                    >  1:415384/1‑251 (MQ=255)

                                                                                                                                                acaATATTCGGCGCGCCCAGCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTg                                                                                                   <  1:410269/251‑1 (MQ=255)

                                                                                                                                                 caATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCTCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTgc                                                                                                  <  1:253259/251‑1 (MQ=255)

                                                                                                                                                    tatTCGGCGCGCCCCTCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGCTCACGCTGCCAAGCTGGTAAGATTCCGCACCGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGCCGGCGCTGGATTCGCCATTCGGCTGCGCCCACCGTGCGGCGAGCGCCAGTTGCCCTTCTCCGTACTGCTCCCTCTgcgcc                                                                                               >  2:178551/1‑250 (MQ=255)

                                                                                                                                                     atTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCAt                                                                                              <  1:108127/251‑1 (MQ=255)

                                                                                                                                                        cGGCGCGCCCACCAGCTCGTTCATGCCATGCGTGCGCGAGGCTGCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATGTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACGGCTGCATCTGCGCATCAg                                                                                           <  2:96227/251‑1 (MQ=255)

                                                                                                                                                                ccATCAGAACGTTCATGACATGCTTGCGCGAGGCTACCGCCGCTTCGCACATGGTGGGTAATTCGGCGATCACGCTGCCAAGCTGATAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGAGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTAGGATGCGACCAGCGTGCGGCGAGCGCCAGTTGATCTTCTTCGTACTGCTGCATCTGCGCTTCAGTGAggg                                                                                     >  2:504098/1‑249 (MQ=255)

                                                                                                                                                                 caccAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGAATCTGCGCATCAGTGAGGGAGt                                                                                  <  1:114625/248‑1 (MQ=255)

                                                                                                                                                                     aGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTAttt                                                                              >  2:396783/1‑251 (MQ=255)

                                                                                                                                                                       cACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGc                                                                            <  1:538062/251‑1 (MQ=255)

                                                                                                                                                                        aCGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGATGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGGGAGGGCGTATTTGcc                                                                           >  1:226713/1‑251 (MQ=255)

                                                                                                                                                                            tCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCGGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTGTCGCGCGCGGCACAGGGCGGCGATGGAGTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTg                                                                        <  2:518165/250‑1 (MQ=255)

                                                                                                                                                                              atgccatgcTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGGGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGat                                                                      >  2:303648/1‑250 (MQ=255)

                                                                                                                                                                                gccatgcTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGataat                                                                   >  1:131435/1‑251 (MQ=255)

                                                                                                                                                                                     gcTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTcg                                                              >  1:356243/1‑251 (MQ=255)

                                                                                                                                                                                         gcgcGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGAt                                                          <  1:17041/251‑1 (MQ=255)

                                                                                                                                                                                                     cgccgcTTCGCACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGCTTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGTTAATATTCGCGATACTTCTCGCGGt                                              >  2:529981/1‑251 (MQ=255)

                                                                                                                                                                                                      gccgcTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGtt                                             <  1:480995/251‑1 (MQ=255)

                                                                                                                                                                                                       ccgcTTCGAACGTGGTGGGAAATGCGGCGATCACGCTGCAAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGAGGGATTCGCGATTCGGCTGCGACCAGTGTGCGGCGAGCGCCAGTTGCTCTTCGTCGTACTGCTGCATCTGCGCATCAGGGAGGGAGTAGTTGCCCTGATAATATTCGCGATACTTCTCCCGGTTg                                            <  2:49992/251‑1 (MQ=255)

                                                                                                                                                                                                           ttCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGATGCTCTTCTTCTTACTGCTGCATCTGCGCATCAGTGCGGGAGTATTTGACCTGATAATCTTCGCGATACTTCTCGCGGTTGGCg                                         >  2:71338/1‑250 (MQ=255)

                                                                                                                                                                                                                aCGTGGTGGGAAATTCGGCGATCCCGCTGCCAAGCTTGGAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGTTGCATCTGCGCATCATTGAGGGAGTATTTGCCCTGATAATATGCGCGATACTTCTCGCGTTTGGCGAACTgg                                   <  2:377000/251‑1 (MQ=255)

                                                                                                                                                                                                                  gttgtgtgaaatgcgtcgtgcACGCTGCCATGCTGGTAAGATGCAACATCGGGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCAGCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGgcg                                 <  2:11580/231‑1 (MQ=255)

                                                                                                                                                                                                                   tggtggGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCATGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGCATTAGCCCTGATAATATTCACGATACTTCTCGCGGTTGCCGAACTGgcgc                                >  2:257854/1‑251 (MQ=255)

                                                                                                                                                                                                                        ggAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTAGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGcc                           >  1:547920/1‑251 (MQ=255)

                                                                                                                                                                                                                         gAAATTCGGCGATCACGCTGCCAAGCTGGAAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGccc                          >  2:294283/1‑251 (MQ=255)

                                                                                                                                                                                                                          aaaTTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCg                         >  2:459270/1‑251 (MQ=255)

                                                                                                                                                                                                                           aaTTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCgg                        >  1:480724/1‑251 (MQ=255)

                                                                                                                                                                                                                              tCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCgg                     >  1:251471/1‑250 (MQ=255)

                                                                                                                                                                                                                               cGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCg                      >  1:242301/1‑249 (MQ=255)

                                                                                                                                                                                                                                  cGATCACGCTGCCAAGCTGGTAAGAGTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACGGGCGCTGGCCCGGCGAGTgg                 <  2:251471/251‑1 (MQ=255)

                                                                                                                                                                                                                                    aTCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTc               >  1:470671/1‑251 (MQ=255)

                                                                                                                                                                                                                                    aTCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTc               >  1:332713/1‑251 (MQ=255)

                                                                                                                                                                                                                                     tCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAgg                                                                                      >  2:156109/1‑179 (MQ=255)

                                                                                                                                                                                                                                     tCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCGTCGTACTGCTGCATCTGCGCATCAGTGAgg                                                                                      <  1:156109/179‑1 (MQ=255)

                                                                                                                                                                                                                                      cACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTcc              >  1:404008/1‑250 (MQ=255)

                                                                                                                                                                                                                                       aCGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATAGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGCTGGATTCGCGATTCGGCCGCGACCAGCGTGCGGAGAGCGCCAGTTGCTCTTCTTAGTACTGCTGCCTCTGCGCATCAGTGAGGGAGTATTTGCACTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCAt            >  1:240027/1‑251 (MQ=255)

                                                                                                                                                                                                                                        cGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCCTCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATc           >  1:349997/1‑251 (MQ=255)

                                                                                                                                                                                                                                         gCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCa          <  1:6886/251‑1 (MQ=255)

                                                                                                                                                                                                                                            gCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATGCTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGc        <  1:189039/250‑1 (MQ=255)

                                                                                                                                                                                                                                            gCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGc        >  2:189039/1‑250 (MQ=255)

                                                                                                                                                                                                                                              cAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGCGac     >  2:208041/1‑251 (MQ=255)

                                                                                                                                                                                                                                                aGCTGGTAAGATTCAGCAACGTGGGAGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTGTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATGCGGCTGCGACCAGCGTGCGGCGAGCGGCAGTTGCTCTTCTTCGTACTGCGGCATCTGCGCATCAGTGAGGTAGTATTTGCCCTGATAATAATCTCGAGGCTGCTCGCGGTTTGCGGACTGGCGCTGGCCCGGCGAGTGGTCCATCGGCGacac   <  2:79789/251‑1 (MQ=255)

                                                                                                                                                                                                                                                   tGGTAAGATTCAGCAAAGCGGGTGAGGGACGCGTCATCGTGGCTGCCAAGAGAAATTTTTAGCGCGCGGCACAGGGAGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGAGGCGAGCGCCAGTTGCGCTTCTTCGTACTGCTGCATCTGAGCATCAGTGCGGGAGAATTTGCCCTGATAATATTCGAGATACTTCTCGCGGTTGGCGCACTGGCCCTGGCCAGGCGAGTGGTCCATCAGCGCCAcc  >  2:517136/1‑249 (MQ=255)

GTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGCGACACC  >  NC_000913/4316251‑4316742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`