Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,296	C→T	S233L (TCG→TTG)	yjbM →	DUF2713 domain‑containing protein YjbM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,296	0	C	T	100.0%	26.3 / NA	9	S233L (TCG→TTG)	yjbM	DUF2713 domain‑containing protein YjbM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

GGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTG  >  NC_000913/4261064‑4261527                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        ggAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCGCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATTGGAGAAATAATATGTGaa                                                                                                                                                                                                                       <  1:48038/251‑1 (MQ=255)                                                                                                                                                   gtaGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTATTTTATGATTTGTTCGGGGTTAAATTGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCAAATATTTATTAGGTTCTTTTTTCTATATCAATACTATTAGCAGGGAGATCTATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATc                                                                     >  2:160883/3‑251 (MQ=255)                                                                                                                                                     aGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATTGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCAt                                                                   <  1:332749/251‑1 (MQ=255)                                                                                                                                                             gACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATTGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCtt                                                            <  1:76943/250‑1 (MQ=255)                                                                                                                                                                                                         tAAATTAATTTATGATATGTTCGGGGTTAAATTGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTCTCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTg                  >  2:378512/1‑248 (MQ=255)                                                                                                                                                                                                                  ttATGATTTGTTCGGGGTTAAATTGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCCCTTCGTGAGAGCTTCATGc      >  2:264374/1‑251 (MQ=255)                                                                                                                                                                                                                      gATTTGTTCGGGGTTAAATTGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTg  >  1:116077/1‑251 (MQ=255)                                                                                                                                                                                                                        tttGTTCGGGGTTAAATTGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGatatat                                                                                                                           >  1:465115/1‑128 (MQ=255)                                                                                                                                                                                                                        tttGTTCGGGGTTAAATTGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGatatat                                                                                                                           <  2:465115/128‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        GGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTG  >  NC_000913/4261064‑4261527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑