Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	416,276	G→A	A226V (GCA→GTA)	sbcD ←	ATP‑dependent structure‑specific DNA nuclease ‑ SbcD subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	416,276	0	G	A	100.0%	197.2 / NA	56	A226V (GCA→GTA)	sbcD	ATP‑dependent structure‑specific DNA nuclease ‑ SbcD subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/35);  total (21/35)

AGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCGGTAATCGCTGCCAGTAAATGCTGCTGTTTTTCAATACCGT  >  NC_000913/416033‑416519                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    agccagACAGGTGGCTCCTGCGATACATCGCGCCACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTg                                                                                                                                                                                                                                              <  2:443656/251‑1 (MQ=255)  gccagAAAGGTGGCTCCTGCGATACATCGCGCCACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGATTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTAACGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTg                                                                                                                                                                                                                                              >  1:215821/1‑250 (MQ=255)   ccagACAGGTGGCGCCTGCGATACATCGCGCCACTGTACCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACTCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCTCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGaa                                                                                                                                                                                                                                            <  1:35144/251‑1 (MQ=255)     agACAGGTGGCTCCTGCGATACATCGCGCCACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAat                                                                                                                                                                                                                                           >  1:292180/1‑250 (MQ=255)            tGGCTCCTGCGATACATCGCGCCACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTAAGTTAAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCCGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCg                                                                                                                                                                                                                                    >  2:331396/1‑250 (MQ=255)              gCTCCTGCGATACATCGCGCCACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGAACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAgc                                                                                                                                                                                                                                 <  1:377712/251‑1 (MQ=255)                           aTCGCGCCACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGCGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCgg                                                                                                                                                                                                                    >  2:383143/1‑251 (MQ=255)                             cgcgCCACGTTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCt                                                                                                                                                                                                                  <  2:292180/251‑1 (MQ=255)                              gcgcCACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtg                                                                                                                                                                                                                 <  2:87060/251‑1 (MQ=255)                                  cACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtggtgg                                                                                                                                                                                                             >  1:355944/1‑251 (MQ=255)                                         ccAGCTGGGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtt                                                                                                                                                                                                       <  1:225027/250‑1 (MQ=255)                                          cAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtttt                                                                                                                                                                                                     >  1:44544/1‑251 (MQ=255)                                                    gTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATGTGCCGTTTGAAAATGGCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCAGGCCGCCAATAAGCTGTACGCGGGTAATATGCCCGAGCGCGATGTAGTCGGCTGGGTGAAAGTTTTGGGCCGGaaa                                                                                                                                                                                           <  2:541973/251‑1 (MQ=255)                                                             gCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAg                                                                                                                                                                                  <  2:140593/251‑1 (MQ=255)                                                                 gATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCCGATGGACATATTTACTCTTACCGCATTCATCAACACTCAGTGGAGTGGGGGAGCCGCACTAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCAGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTg                                                                                                                                                                              >  2:212841/1‑251 (MQ=255)                                                                    cGCCTTTCAGCACTGCTATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGGTCCATGTCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCa                                                                                                                                                                           <  1:380460/251‑1 (MQ=255)                                                                              cACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGt                                                                                                                                                                 <  2:113739/251‑1 (MQ=255)                                                                                cTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCACTAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTcc                                                                                                                                                               >  2:208810/1‑250 (MQ=255)                                                                                     aTGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTcacgcac                                                                                                                                                          <  1:456276/251‑1 (MQ=255)                                                                                                    ggTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACt                                                                                                                                           <  2:446023/251‑1 (MQ=255)                                                                                                                        gCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATAGTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAAGAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAGATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAAt                                                                                                                       <  2:76607/251‑1 (MQ=255)                                                                                                                         ctctctAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATg                                                                                                                      >  1:276681/1‑251 (MQ=255)                                                                                                                               aaTTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTccc                                                                                                                  <  1:321283/249‑1 (MQ=255)                                                                                                                                  ttGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTGTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTgg                                                                                                              <  2:103221/250‑1 (MQ=255)                                                                                                                                    gCCGTTTGAACATGTCACCCGATTGCCATATTTACTCTTCCCTCTTTCATCCAAACGCCGTGTAATGGTGTAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCTCGATGTAGTCTTCTGGTTGTCATTTTTTTTCCCTACACGCGTCCAGCGTGCCAATATAAATCTCTCGCACGGCGTCTCTTTTACTGGCCCCCACTGTCCTTATATGTCCCGTGGCg                                                                                                            <  2:361576/250‑1 (MQ=255)                                                                                                                                    gCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAAGCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCg                                                                                                            <  2:257432/250‑1 (MQ=255)                                                                                                                                      cGTTTGAAAATGTCACCAGATGGCCATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCAATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCCGCGTGCCAATCTAACTGTCACGCACGGCGTCACTTTTACTGGCCCACACGGTCGTTACATGTCCCGTGGCgttg                                                                                                         >  2:70473/1‑248 (MQ=255)                                                                                                                                       gTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACGCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCgatga                                                                                                        <  2:44544/251‑1 (MQ=255)                                                                                                                                           gAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATgg                                                                                                     <  1:429825/250‑1 (MQ=255)                                                                                                                                           gAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTGTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATggg                                                                                                    <  1:7201/251‑1 (MQ=255)                                                                                                                                               aTGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGATAGTTTTGTGCCGGAAACGCGTCCACCGTGCCCATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGTTGATGGGc                                                                                                   >  1:549465/1‑248 (MQ=255)                                                                                                                                                 gTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGAAGAgg                                                                                              >  2:435461/1‑251 (MQ=255)                                                                                                                                                  tCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGCATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGCTGCTGGGCAGAGGc                                                                                             >  2:126423/1‑251 (MQ=255)                                                                                                                                                    aCCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTg                                                                                           <  1:140193/251‑1 (MQ=255)                                                                                                                                                             aCATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCCGAGGCTGATCGCcgcg                                                                                  >  2:347831/1‑251 (MQ=255)                                                                                                                                                                tatTTCCTCTTACCGCATTCATAAAAACTCCGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCgcgc                                                                                 >  2:243231/1‑249 (MQ=255)                                                                                                                                                                 atTTACTCTTACCTCATTCATCAAAACTCAGTGGAAGGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGGGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGGCGGTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAg                                                                               <  2:551861/250‑1 (MQ=255)                                                                                                                                                                     aCTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGAACGCCGCGCAGTTTGc                                                                          >  2:323987/1‑251 (MQ=255)                                                                                                                                                                          tACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGc                                                                      <  1:435461/250‑1 (MQ=255)                                                                                                                                                                          tACCGCATTCAGCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCTGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCTCGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGc                                                                      <  2:447826/250‑1 (MQ=255)                                                                                                                                                                               cATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGc                                                                                                             >  2:186900/1‑206 (MQ=255)                                                                                                                                                                               cATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGc                                                                                                             <  1:186900/206‑1 (MQ=255)                                                                                                                                                                                  tcatcaAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCAt                                                              >  1:313893/1‑250 (MQ=255)                                                                                                                                                                                        aaaCTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGtgttg                                                       <  1:8738/251‑1 (MQ=255)                                                                                                                                                                                                gggAATGGGGGAGCCGCAAGAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGtaat                                               <  1:383143/250‑1 (MQ=255)                                                                                                                                                                                                     ggggggAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCg                                          <  1:152773/250‑1 (MQ=255)                                                                                                                                                                                                         ggAGCCGCAATAGCGAACATGTTACATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGAGGACAGTTTTTTGCCGGAAAAGCCTCCAGAGTGCCACTATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAAAGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATCGTGTTGTTGGTACTAATCGGTa                                       >  1:70662/1‑250 (MQ=255)                                                                                                                                                                                                          gAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATcgccg                                                                                    <  2:13869/204‑1 (MQ=255)                                                                                                                                                                                                          gAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATcgccg                                                                                    >  1:13869/1‑204 (MQ=255)                                                                                                                                                                                                           aGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCGGTAATc                                    <  1:107462/251‑1 (MQ=255)                                                                                                                                                                                                                    aGCGAACATGTTCCATGCCGCCAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCGGTAATCGCTGCCAGt                           <  2:302903/251‑1 (MQ=255)                                                                                                                                                                                                                                     ccgccAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACTCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCGGTAATCGCTGCCAGTAAATGCTGCTGTTTTTc          <  1:315002/251‑1 (MQ=255)                                                                                                                                                                                                                                      cgccAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCGGTAATCGCTGCCAGTAAATGCTGCTGTTTTTCa         <  1:70473/251‑1 (MQ=255)                                                                                                                                                                                                                                         cAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCgatgat                                                                                                       >  1:126880/1‑154 (MQ=255)                                                                                                                                                                                                                                         cAATAATCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGGCGTTAAATGTCCCGTGGCgatgat                                                                                                       <  2:126880/154‑1 (MQ=255)                                                                                                                                                                                                                                             aatCTGTACGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACGGGCCCCCACGTTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCGGTAATCGCTGCCAGTAAATGCTGCTGTTTTTCAATACCGt  <  1:257876/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    AGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACTGTTCCAGCTGTGCGGTAATCGACGCCAGATCGCCTTTCAGCACTGCCATGGGTTGCGTTACCGGTACGTTCAGGTTTTCCACGCTCTCTAATTTGCCGTTTGAAAATGTCACCAGATGGACATATTTACTCTTACCGCATTCATCAAAACTCAGTGGAATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGTCACGCACGGCGTCACTTTTACTGGCCCCCACGGTCGTTAAATGTCCCGTGGCGATGATGGGCAGAGGCTGATCGCCGCGCAGTTTGCAGGCATCGGCATAGTGTTGTTGGTAATAATCGGTAATCGCTGCCAGTAAATGCTGCTGTTTTTCAATACCGT  >  NC_000913/416033‑416519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑