Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,638	T→C	intergenic (‑10/‑190)	iclR ← / → metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,638	0	T	C	100.0%	169.7 / NA	47	intergenic (‑10/‑190)	iclR/metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/34);  total (13/34)

GTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGT  >  NC_000913/4223394‑4223880                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ggtCGCCAACCTGACGCACGAACCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTc                                                                                                                                                                                                                                               <  2:98207/249‑1 (MQ=255)    cGCCAACCTGACGCTCGAATCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGt                                                                                                                                                                                                                                            <  1:268297/251‑1 (MQ=255)        aaCCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCCGTTT‑GCGGGGATGGGGGCGGCCCTGACAGTCTCCTTTTTTTGttttt                                                                                                                                                                                                                                        >  2:393256/1‑247 (MQ=255)          ccTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTg                                                                                                                                                                                                                                      <  1:86157/251‑1 (MQ=255)             gACGCACGAAGCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGTCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTGTCTGCTGT‑GTTTCGCGGGAATGAGTGCGACCATTACAGTCTCCTTTTTCTGTATCGTGGaa                                                                                                                                                                                                                                   <  2:190971/251‑1 (MQ=255)               cGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTATCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAt                                                                                                                                                                                                                                 <  1:273977/251‑1 (MQ=255)                    gAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGACACGAGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGC‑GGTTTGCGGGAATGGGGGCGCCCATGACAGTCTCCTCTTTCTTTATTGGGGAAAtgaatt                                                                                                                                                                                                                            >  1:502696/1‑246 (MQ=255)                       aCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttca                                                                                                                                                                                                                         <  2:2129/251‑1 (MQ=255)                                 tGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTg                                                                                                                                                                                                                <  1:31783/250‑1 (MQ=255)                                  gCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTGCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGt                                                                                                                                                                                                               <  1:454501/250‑1 (MQ=255)                                         ggTTAGCAGGCGGTGTGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGGAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTaa                                                                                                                                                                                                        <  1:516678/250‑1 (MQ=255)                                          gTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTGCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCGAATg                                                                                                                                                                                                      <  2:91107/251‑1 (MQ=255)                                              gCAGGCGGTGGGTCGTGGAATTGGGTAACCCGTCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGTGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                                                                                                                                                                                  <  1:243872/251‑1 (MQ=255)                                               cAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGC‑GGTTCGCGGGAATGGGGGCGGCCATTGCAGTCTCCTTTTTTTGTATCGTGGGAATCATTTTCCTTTTTTTTGTTAGCTAATGCAAt                                                                                                                                                                                                  >  1:20964/1‑250 (MQ=255)                                                  gCGGTGGGTCGTGGGTTTGGGTAACCCGGCGTGTTGCGCGAGTTCCGTGAGTGCCACCCTGGCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACTTGTCCAGTCGCTGGTGCTGTGGCAATGGCGGGTTTTCTGCCGC‑TTTTCGCGGGAATGCGTGCGACCATGACAGTCTCCTATTTCTGTATCGTGGAAATCATTTTTATTTTTATTGTTAGCTAATGCAATAGt‑t                                                                                                                                                                                             <  1:489759/251‑1 (MQ=255)                                                    ggTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTaa                                                                                                                                                                                                        <  1:459609/239‑1 (MQ=255)                                                           cGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑Tactgaact                                                                                                                                                                                     <  1:274878/250‑1 (MQ=255)                                                           cGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCGCCTTTTTCTGTATCGTTGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑Tactgaactg                                                                                                                                                                                    <  1:231079/251‑1 (MQ=255)                                                               gAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGTATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCAGTTTCATTTGTATTGTTAGCTAAGTCAATAGT‑TACTGAACTGATcc                                                                                                                                                                                <  2:107241/251‑1 (MQ=255)                                                                     ggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAg                                                                                                                                                                          <  1:308017/251‑1 (MQ=255)                                                                            cGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGt                                                                                                                                                                   <  1:336994/251‑1 (MQ=255)                                                                                     gcgcCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAAt                                                                                                                                                          <  1:105582/251‑1 (MQ=255)                                                                                        ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAAtctc                                                                                                                                                       <  1:256746/251‑1 (MQ=255)                                                                                            ttCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGC‑GTTTTGCGGGAATGGGGGCGACCCTGACAGACTCCTTTTTTTGTATCGTGGGAATCATTTTTATTTTTTTTTTTTGCTTATGCAATAGT‑TACTTAACTGTTTCGATGGGTTAATGTTTAACAAATCTCAttt                                                                                                                                                   >  1:12449/1‑249 (MQ=255)                                                                                                gTGAGTGCCGCACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACGGAACCTGTCCAGTCTCTGGTGCGGTGGCAACGGCGGGTTTGCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATGGTTAGCTAATGGAATGGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCg                                                                                                                                               <  1:41060/251‑1 (MQ=255)                                                                                                gTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGC‑GTTTTGCGGGAATGGGGGGGGCCCTGACAGTCTCCTTTTTTTGTTTCGTGGGAAACATTTTTATTTTTTTTTTTTTCTAATTCACTAAT‑TACTGAAATGATCCGCTGAGGTAATTTTGAGAAAATCTCATGTTgtg                                                                                                                                               >  2:341781/1‑249 (MQ=255)                                                                                                              gCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCtt                                                                                                                                   <  1:341781/249‑1 (MQ=255)                                                                                                                   tGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACca                                                                                                                             <  1:409082/250‑1 (MQ=255)                                                                                                                                cctcTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAAGCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATg                                                                                                               <  2:18865/248‑1 (MQ=255)                                                                                                                                    tCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGC‑GTTTCGCGGGAAAGGGGGCGACCCTGAACGTCTCCTTTTTTTGTATCGTGGACATCATTTTCATTTTTATTTTTTGCTAATTCCATAGT‑TACTTAACTGATCCCATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTTCCACAGAAGCGttttttcc                                                                                                             >  1:64993/1‑244 (MQ=255)                                                                                                                                          aaTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCATCGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAGTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGt                                                                                                     <  2:6493/251‑1 (MQ=255)                                                                                                                                               cAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGGGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTttgt                                                                                                 <  1:249449/250‑1 (MQ=255)                                                                                                                                                                aCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCt                                                                               <  2:98581/251‑1 (MQ=255)                                                                                                                                                                                              cAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACATTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGTTTGAGCGTGTCGGGAGCAAGTGTGagc                                                 <  1:407721/251‑1 (MQ=255)                                                                                                                                                                                                 cggcggGTTTTCTGCCGC‑GTTTCGCGGGGATGGGTTCGAACATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTTTTAGCTATTTCCATAGTATA‑TGAACTGGTCCCATGAGTTAATGTTTAACCCATCTCCTGGTGCGTGGGGGTCGCTTTTACCACAGATGCGTTTATTCCAGTTTGGTTTGTTGGATTTTTATTAAATTTTGGTTGGGCGTGTCGGGGGCAAGTGTGagcagc                                              >  2:91602/1‑251 (MQ=255)                                                                                                                                                                                                 cggcggGATTTCTGCCGC‑GTTTCGAGGGACTGGGTGCGACCATGGCAGTATCCTTTTTCTGTATCGTGGAAATCATTTTACTTTTTATTTTTTGCTAATGCAATCGT‑TACTGACCTTATCCGATGAGTTTATGTTGAACAAATCTCATGTTGCGTGGGGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTTAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGggcagc                                              >  2:534819/1‑251 (MQ=255)                                                                                                                                                                                                      ggTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGt                                         <  1:393256/251‑1 (MQ=255)                                                                                                                                                                                                                 gc‑gTTTCGCGTGAATGGGGGCGACCATGACAGTCTCCTTTTGCGGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAGGCAATAGT‑TACTGAACTGATCCGATGAGGTAATGTTGAACAAATCTCATGTTGCGTTGTGGTCGCTGTTACCACAGATGTGTTTATGCGAGGATGGTTGGGTGAATCGTTATTAAAGCTGGGTTGAGCGTGTCGGGAGCAAGGGGGAGCAGCAAAGTGGATCTACtgc                              <  2:280005/251‑1 (MQ=255)                                                                                                                                                                                                                 gc‑gTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgc                              >  1:6469/1‑251 (MQ=255)                                                                                                                                                                                                                    gTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgt                            <  2:138888/251‑1 (MQ=255)                                                                                                                                                                                                                              aaTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTa                   >  1:273061/1‑250 (MQ=255)                                                                                                                                                                                                                                   gTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTTAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGaa             <  2:33913/251‑1 (MQ=255)                                                                                                                                                                                                                                       gACCACGACAGTCTCCTTTTTCTGCATCGTGGACATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACACATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCCAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGt          >  2:270032/1‑250 (MQ=255)                                                                                                                                                                                                                                        aCCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTAt        <  1:551708/251‑1 (MQ=255)                                                                                                                                                                                                                                              aCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgc                              <  2:244553/223‑1 (MQ=255)                                                                                                                                                                                                                                              aCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgc                              >  1:244553/1‑223 (MQ=255)                                                                                                                                                                                                                                              aCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAAGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTggg  >  2:39165/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGC‑GTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGT‑TACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGT  >  NC_000913/4223394‑4223880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑