Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,670	G→T	D65Y (GAT→TAT)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,670	0	G	T	100.0%	126.5 / NA	36	D65Y (GAT→TAT)	aceB	malate synthase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/15);  total (21/15)

GTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTT  >  NC_000913/4215440‑4215913                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gTGGGGAAGTTTTCGGATACATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATCTTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCt                                                                                                                                                                                                                                 >  2:79538/1‑251 (MQ=255)         gTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTcg                                                                                                                                                                                                                         >  1:134721/1‑251 (MQ=255)              cGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATg                                                                                                                                                                                                                    <  1:249397/251‑1 (MQ=255)                                        tGACTGAACAGGCAACAACCACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAACTTCTTACTGCCGAAGCGGTAGAATTTCTGAATGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTATGGCAGAGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCt                                                                                                                                                                                          >  2:215043/1‑251 (MQ=255)                                          aCTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCTGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGc                                                                                                                                                                                        <  1:525578/251‑1 (MQ=255)                                               aCAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACt                                                                                                                                                                                   >  1:7533/1‑251 (MQ=255)                                                       caacaacCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATTAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGAc                                                                                                                                                                           >  1:550116/1‑251 (MQ=255)                                                                       ggCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGAt                                                                                                                                                             <  1:364716/249‑1 (MQ=255)                                                                                  ggCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGt                                                                                                                                                 >  2:141201/1‑250 (MQ=255)                                                                                                    agaAGCAAATTCTTACTGCCGAAGCGTTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACATCGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCCGAATGGAAAATTCGCGGGATTCCTGCGGACTGAGAAGACCGCCGCGTAGAGATAACTGTCCCGGTAGAGCGCAAGATGTTGATc                                                                                                                              <  2:550116/251‑1 (MQ=255)                                                                                                                       cGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAtg                                                                                                           >  2:427961/1‑251 (MQ=255)                                                                                                                        gAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCACtgt                                                                                                          >  2:136105/1‑251 (MQ=255)                                                                                                                             ggTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAg                                                                                                     <  2:7533/251‑1 (MQ=255)                                                                                                                               tAGAGTTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTACATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCCCAACGCCAGTGTGAAAGTc                                                                                                   >  2:216375/1‑251 (MQ=255)                                                                                                                                    tttCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTAt                                                                                              >  1:194006/1‑251 (MQ=255)                                                                                                                                     ttCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGGCTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTAt                                                                                              <  1:90631/250‑1 (MQ=255)                                                                                                                                         gactgaGCTGGAGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTCTTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCACCGCCAATGTGCAAGTCTTTATGGCCg                                                                                         >  2:345045/1‑251 (MQ=255)                                                                                                                                                    tGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGa                                                                                  >  1:388379/1‑247 (MQ=255)                                                                                                                                                         cATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACt                                                                         <  2:277789/251‑1 (MQ=255)                                                                                                                                                               aCGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCAc                                                                    <  1:427961/250‑1 (MQ=255)                                                                                                                                                                cGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCAcc                                                                   >  1:428167/1‑250 (MQ=255)                                                                                                                                                                   cacgaCGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGAc                                                               >  1:196336/5‑251 (MQ=255)                                                                                                                                                                                   tCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCGCCAGACTGGAACAAAGTGATc                                                <  2:376085/250‑1 (MQ=255)                                                                                                                                                                                   tCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGAt                                                 <  2:272976/249‑1 (MQ=255)                                                                                                                                                                                      ggCAGCGCGCATTCATCAGCAGCAAGATATTGATCACGGAACGTTGCCTTATGTTATTTCGGAAACAGCTGCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATTTGAAAGTCTTTATGGCCGATTTCGAATATTCACTGGCTCCAGACTGGAACAAAGTGATCGACg                                            <  2:14867/251‑1 (MQ=255)                                                                                                                                                                                          gcgcgcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCa                                        <  1:99197/251‑1 (MQ=255)                                                                                                                                                                                           cgcgcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGATGGGCaa                                       <  2:388379/251‑1 (MQ=255)                                                                                                                                                                                                    agcagcagcAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTg                              >  2:172831/1‑251 (MQ=255)                                                                                                                                                                                                              aGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGACGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGAGAAAGTCTTTATGTCCGATTTCGAAGATTCACTGGCACCa                                                                  >  1:181721/1‑205 (MQ=255)                                                                                                                                                                                                              aGATATTGATAACGGAACGTTGCCTTATTTTAGTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCATCGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCa                                                                  <  2:181721/205‑1 (MQ=255)                                                                                                                                                                                                                   ttGATAGCGGAACGGTGCCTTATTTTATTGCGGAAACAGCTTCCATTAGCGATGCTGATTGGAAAATTCGCGGAATTCCTGCGGACTTAGAAGACAGCCGCGTAGAGATAACTGGCACGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCCAGGTGGAAGTCTTTATGGCCGAGTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAAc               >  2:34248/1‑251 (MQ=255)                                                                                                                                                                                                                   ttGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGTGATTCCTGCGGACTTAGAGGACCGCCGCGTAGAGATAACTGGCCCGTTAGCGCGCAAGAGGGTGATCAACGCGCTCAACGCCAATGGGAAAGCCTTTATGGCGGATTTGGAAGTTTCCCTGGCACCAGACGGGACAAAAGAGATCAACGGGCAAATTAACTTACGGGATGCGGTTaa                >  2:411758/1‑250 (MQ=255)                                                                                                                                                                                                                           ggaccGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCACGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACACCAATGTGACAGTCTTTATGGACGATTTCGAAGATTCACTGGCACCAGACTGGAACAAATTGATCGACGGGCCAATTAACCTGCGAGATGCGGTTAACGGCACCAt       >  2:523770/5‑251 (MQ=255)                                                                                                                                                                                                                           ggAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCAt       >  1:170564/1‑251 (MQ=255)                                                                                                                                                                                                                              aCGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAg    <  1:74496/251‑1 (MQ=255)                                                                                                                                                                                                                                 ttGCCTTATTTTATTTCGGACACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGCCCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCACCGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGtt  >  1:450060/1‑250 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTT  >  NC_000913/4215440‑4215913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑