Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,707,9470CA92.9% 85.4 / NA 28intergenic (‑242/+669)dppA/proKdipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (10/16);  total (12/16)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATAA  >  NC_000913/3707702‑3708189
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  
gCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCacaaacaa                                                                                                                                                                                                                                                >  1:487944/1‑250 (MQ=255)
               aaTTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTc                                                                                                                                                                                                                                <  1:17261/251‑1 (MQ=255)
                      atgtttgtagaaatatttccctGCAGTTGGTAGTCGCGGTCTGTCCTTTTTTGCTTTTTGCCCGGTCGG‑TCGAACGTTAGGAGGTGCGGCACCCTAATTAATTC‑CACTAAGTGTAACAGTGCGGATCCTCCATAAAATGACCCTCGTGACCTACAATCTGTCAACAGACTGTGAAAACGCTCA‑TACAGCCAACCGGGATTTACACCAACGGTGTAATTCAAGACACAAAGATTACAAAAACTTCAAAc                                                                                                                                                                                                                            >  2:475133/22‑248 (MQ=255)
                        gtttgttGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTa                                                                                                                                                                                                                       <  1:311808/251‑1 (MQ=255)
                            gttgttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGCGGTGGGGATGCCGTACTAATTCACA‑TCAGTGTAACAGTGCGGATCCTCCATAACATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAACCG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACACAGATTAAAAAAACTTCAAACAGCTAttcg                                                                                                                                                                                                                   >  1:226889/1‑249 (MQ=255)
                            gttgttTTAACCCTTTGCAGTGGGTTGTCGCTGCCGGACCTGTGTTGTTGTTTGCCCGTGCGGGTC‑AACGTTATGAGGTTGGGATGCCGTACTAATTAACA‑TCAGTATAACAGTGCGGAGCCTCCATAAAATGCCCCGCGTGACCTACAAGCTGACAACAGAATGTGAAAACG‑TCAATACATCGAGCCGGGATTTACACCAGCGGTGAGAATCCACAAACAGAGATTAAAAAATCTTCAAACAGCTAtt                                                                                                                                                                                                                     <  2:226889/249‑1 (MQ=255)
                                      cccTTTGCAGTGGGTTGTCGCTGCCTGGCCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAAGCCACAAACAAAGATTAAAAAAACTTCAAACAGCGATTTGCAGCAGAGaa                                                                                                                                                                                                         <  1:179899/251‑1 (MQ=255)
                                      cccTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGCGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACCGAATGTGAAAACG‑TCAATACAGCAAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAgaga                                                                                                                                                                                                          >  1:298000/1‑250 (MQ=255)
                                               gTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCt                                                                                                                                                                                                >  1:116304/1‑251 (MQ=255)
                                                         gCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAt                                                                                                                                                                                      >  1:72134/1‑251 (MQ=255)
                                                            gCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATg                                                                                                                                                                                     <  1:16140/249‑1 (MQ=255)
                                                            gCCTGACCGTTTTTGTTTGTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACATCTATTTGCAGCAGATAAATTTGTGCTCCGCCAACATGa                                                                                                                                                                                    <  2:88823/250‑1 (MQ=255)
                                                                gACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAg                                                                                                                                                                                <  1:201419/250‑1 (MQ=255)
                                                                                  cccGGTCGGGTC‑ACCGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGAGTAACAGTGCGGATCCTCAATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGACAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACCAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATAttt                                                                                                                                                             >  1:72994/1‑251 (MQ=255)
                                                                                               aaCGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTgg                                                                                                                                                 <  1:133265/251‑1 (MQ=255)
                                                                                                       gAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAGACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAg                                                                                                                                         <  1:72768/251‑1 (MQ=255)
                                                                                                        aGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACATCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCATCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGt                                                                                                                                        <  1:127451/251‑1 (MQ=255)
                                                                                                                  gCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAGTTGGTGATTAAGTTTTATGCAt                                                                                                                               <  2:487944/250‑1 (MQ=255)
                                                                                                                             tAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCACTACAGCCAACCGGGATTTACACCCACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCt                                                                                                                                                                     >  2:279483/1‑201 (MQ=255)
                                                                                                                             tAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCGATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCt                                                                                                                                                                     <  1:279483/201‑1 (MQ=255)
                                                                                                                                                          cATAAAATGCCCCTCGTGACCTACAATCTGTCAACCGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCg                                                                                       >  2:67842/1‑251 (MQ=255)
                                                                                                                                                                            aCCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCt                                                                     <  2:72134/251‑1 (MQ=255)
                                                                                                                                                                                             aGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGaa                                                     >  2:119809/1‑250 (MQ=255)
                                                                                                                                                                                                        aCG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAAGCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGGGGATATCTGAGCGATTAATGCCATGAGCGCTAAAACGCACAGCGGAACATGCCAGGgcc                                         <  2:193197/251‑1 (MQ=255)
                                                                                                                                                                                                         cG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGgcca                                        <  2:376217/251‑1 (MQ=255)
                                                                                                                                                                                                                                  acacCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCaaaaaa                    >  2:7689/1‑247 (MQ=255)
                                                                                                                                                                                                                                        aCGGTGAGAATCCACAAACACAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGc          >  2:87534/1‑251 (MQ=255)
                                                                                                                                                                                                                                          ggggagaATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCa         >  2:422269/4‑250 (MQ=255)
                                                                                                                                                                                                                                                aaTCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATaa  <  2:175153/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  
GCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTC‑AACGTTATGAGGTGGGGATGCCGTACTAATTAACA‑TCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG‑TCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATAA  >  NC_000913/3707702‑3708189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑