Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,362 G→A intergenic (+496/+260) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,3620GA96.1% 298.3 / NA 104intergenic (+496/+260)rtcR/glpGDNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (51/48);  minor base C (0/4);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTC  >  NC_000913/3560116‑3560603
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    
gggAACGGGGAGATAAAATCGGGCGTCGCTCCGCCAATGACCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCAGCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAAGTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGGGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGAt                                                                                                                                                                                                                                                  <  2:464798/250‑1 (MQ=255)
  gAACTGGGAGATAAAATCGAGCGTCGCTGCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCACCGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATgc                                                                                                                                                                                                                                                <  2:154777/251‑1 (MQ=255)
   aaCTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGCTGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCCCTTTGCGGACGATgcg                                                                                                                                                                                                                                               >  1:494359/1‑251 (MQ=255)
    aCTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGG‑TT‑TG‑CTCCGCCACTTTGCGGACGATgcgc                                                                                                                                                                                                                                              >  2:173155/1‑251 (MQ=255)
    aCTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATgcgc                                                                                                                                                                                                                                              >  1:432246/1‑251 (MQ=255)
      tGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGCATGATGCGAAAATCTTCTTTTACCCTCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGctc                                                                                                                                                                                                                                            >  1:272174/1‑251 (MQ=255)
       gggAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGctct                                                                                                                                                                                                                                           <  1:392079/251‑1 (MQ=255)
        ggAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACGTTGCGGACGATGCGCTCtt                                                                                                                                                                                                                                          <  1:484223/251‑1 (MQ=255)
            aTAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTttc                                                                                                                                                                                                                                      >  1:262035/1‑251 (MQ=255)
              aaaaTCGAGCGCCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGAGGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGGGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCTAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTttctt                                                                                                                                                                                                                                    <  2:356695/251‑1 (MQ=255)
                 aTCGGGCGTCGCTTCGCCAATGCCCCAGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTAACATCAACGTGTT‑AGCAAAGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCTGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGCGCCATTGGGGG‑TT‑TG‑CTCCGCCACTTTGCGTACGAGTCCCTCTTTTTCTTCgg                                                                                                                                                                                                                                 >  2:265753/1‑251 (MQ=255)
                   cGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTc                                                                                                                                                                                                                               >  1:22212/1‑251 (MQ=255)
                        gTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGCGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGCTGCGCTCTTTTTCTTCGGTCTgggg                                                                                                                                                                                                                          >  1:219833/1‑250 (MQ=255)
                            cTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGcc                                                                                                                                                                                                                      >  1:168870/1‑251 (MQ=255)
                              tCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGcc                                                                                                                                                                                                                      <  1:112012/249‑1 (MQ=255)
                               cgcaaaTGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCGGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCtt                                                                                                                                                                                                                    <  2:491575/246‑1 (MQ=255)
                               cGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCtt                                                                                                                                                                                                                    <  1:510264/250‑1 (MQ=255)
                                gCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCACCCGGCGAGAAGGAGGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTGGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGc                                                                                                                                                                                                                  <  2:24617/251‑1 (MQ=255)
                                   aaTGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATA‑GAGCAACAGCGTCGAGCCATTGGAGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGCGGCCTTGCGAt                                                                                                                                                                                                               >  2:154179/1‑251 (MQ=255)
                                                 cgcgGCTGCGAAATTCGCCACCGGCGAGAATGAGGCGAAAATCTTCTTTTACCAGCAACGTGTT‑AGCAACTTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGGGGTG‑CCGATATC‑GATAAACAGCGTGGAGCTATGGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCgt‑g                                                                                                                                                                                                <  2:471668/251‑1 (MQ=255)
                                                   cgGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑ATCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGAGGCCTTGCGATCGTGCCACGGCGT‑Gtt                                                                                                                                                                                              >  1:85594/1‑251 (MQ=255)
                                                    ggCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTTTT‑AGCAACGTTGAGATTGTGGGGGACAATGCGCAAATTGCTGGGATGGAGCAGTGCTTGCTCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTa                                                                                                                                                                                             <  2:543544/251‑1 (MQ=255)
                                                    ggCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑Gtt                                                                                                                                                                                              >  1:136386/1‑250 (MQ=255)
                                                    ggCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATAAACGTGTT‑AGCAACGTTGAGATTGTTGGAGAACATGCGAAAATTGCTGCGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGAGTAGACCCATTGGGGA‑TT‑TG‑CTCCGCAACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑Gtt                                                                                                                                                                                              >  1:550337/1‑250 (MQ=255)
                                                     gCTGCGTAATTCGCCACCGGCGAGAATAATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCCACGTTGAGATTGTTGGTGAAAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGC‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTTCGATCGTGCCACGGCGT‑GTTa                                                                                                                                                                                             >  2:366434/1‑250 (MQ=255)
                                                           tAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCgcac                                                                                                                                                                                      >  1:129223/1‑248 (MQ=255)
                                                           tAATTCGCCACCGGCGAGAAGGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTAGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGCGCCATTGGGGA‑TT‑GG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGCTGGCCTTGCGATCGTGCCACGGCGT‑Gttcacagacc                                                                                                                                                                                      >  2:69506/1‑243 (MQ=255)
                                                                cGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGa                                                                                                                                                                                  <  1:34206/250‑1 (MQ=255)
                                                                    cccGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCa                                                                                                                                                                             <  1:55499/250‑1 (MQ=255)
                                                                     ccGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGCCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGc                                                                                                                                                                              >  2:490658/1‑249 (MQ=255)
                                                                       ggCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAgcg                                                                                                                                                                          >  2:402077/1‑251 (MQ=255)
                                                                              atgatgCGAAAATCTTCTTTTCCCAGCAACGTGTT‑AGCAACGTTGAGATTGTTGTTGACAAGGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CGCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCAcc                                                                                                                                                                   <  2:493110/251‑1 (MQ=255)
                                                                              atgatgCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGTGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGACGGCAGcgccgcccc                                                                                                                                                                   >  2:128248/1‑248 (MQ=255)
                                                                              atgatgCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCAcc                                                                                                                                                                   >  1:471452/1‑251 (MQ=255)
                                                                              atgatgCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTGGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTCCCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATGGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCAcc                                                                                                                                                                   <  2:65211/251‑1 (MQ=255)
                                                                               cgaggCGAAAATCTGCTTTTCCCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTCCCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCAcc                                                                                                                                                                   <  2:24281/246‑1 (MQ=255)
                                                                                 atgCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCAcc                                                                                                                                                                   >  1:24281/1‑248 (MQ=255)
                                                                                 atgCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGcc                                                                                                                                                                >  1:8542/1‑251 (MQ=255)
                                                                                 atgCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGcc                                                                                                                                                                >  1:474154/1‑251 (MQ=255)
                                                                                     gAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCatg                                                                                                                                                             >  1:207400/1‑250 (MQ=255)
                                                                                     aaaaaTCTTCTTTTACCATCACCGGTTT‑AGCAGCGTTGAGATTGTTGGGAACAATGCGCAAATTGCTGGGATTGAGCGGTGCGTGCGCTCCCGT‑TCCCGGCGGGGGG‑CCGATATC‑GATAACCGGCGCCGAGCCTTGGGGGA‑TT‑TG‑CCCCGCCACTTGGCGGACGATGCGCTCTTTTCCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACGGGAAGGCAGCGCCGCACCGCCatga                                                                                                                                                            <  2:262035/250‑1 (MQ=255)
                                                                                          ccttcttTTACCATAAACGTGTT‑AGCAACGTTGAGAGTGTTGGTGACAATGCGCAAATTGTTGTGAGTGAGCAGTGCGGGCGCTACCGC‑TCCCGGCGTGGGG‑CCGATATC‑GATAAACAGCGTCGAGCCTTTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑cg                                                                                                                                                      <  1:203943/250‑2 (MQ=255)
                                                                                           cttcttTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑cgc                                                                                                                                                     >  1:429612/1‑249 (MQ=255)
                                                                                           cttcttTTACCACCAAC‑TGTTTAGAAACGTTGAGATTTTGGGTGACAACGTGAATATTGCTGTGATGGAGCAGGGGGTGC‑CCAGCGCCTTCCGCTGGGGGG‑ACGATATC‑GATAACCAGCTGCGATCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTTTTTTCCTTGGGTCTGGGGGGCCTTGCGATCGTTCCACGGCTT‑GTTAACCAAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑c                                                                                                                                                       <  2:383029/249‑1 (MQ=255)
                                                                                                  tACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCAACGCCATGATGG‑CGCAGGATc                                                                                                                                               >  1:30814/1‑250 (MQ=255)
                                                                                                  aaccaccAACGTGTA‑AGCAACGTGGATATTGTGGGTGACAAGGCGAAAATGGCTGTGATGGAGCAGGGCGTGCGCGACCGC‑TTCGGGCGTGGTG‑CCGATACC‑GATAACCAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCAAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCa                                                                                                                                              <  2:71916/250‑1 (MQ=255)
                                                                                                    ccATCAACGTGTG‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑AATAAACAGCGCCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAgg                                                                                                                                            <  1:168819/251‑1 (MQ=255)
                                                                                                             tgtT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTcc                                                                                                                                   >  1:176734/1‑251 (MQ=255)
                                                                                                                    cAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGaa                                                                                                                                                                                       <  1:504210/194‑1 (MQ=255)
                                                                                                                    cAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGaa                                                                                                                                                                                       >  2:504210/1‑194 (MQ=255)
                                                                                                                     aaCGTTGCGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGCTATC‑GATAAACAGCGTCGAGCCCTTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGCTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATc                                                                                                                                                                                                              >  1:127837/1‑171 (MQ=255)
                                                                                                                     aaCGTGGAGATTGTTGGTGACATTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCTCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATc                                                                                                                                                                                                              <  2:127837/171‑1 (MQ=255)
                                                                                                                      aCGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTCCCGT‑TTCCGGGGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                           <  1:324337/251‑1 (MQ=255)
                                                                                                                       cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGATACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                           >  1:302991/1‑250 (MQ=255)
                                                                                                                           gagaTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGAAGCGCCGCCACGCCATGTTGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTgt                                                                                                                      >  1:303015/1‑250 (MQ=255)
                                                                                                                            agtttgtggggaacaaggctcAAATTGCTGGGAGTGAGCGGGTCGTGCGCTCCCGT‑TCCCGGGGTGGGG‑CGGA‑ATCCGAAAAACGGCGGCGAGCCATTGGGGT‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                                     <  2:136386/231‑1 (MQ=255)
                                                                                                                               ttgttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                  >  1:448873/1‑251 (MQ=255)
                                                                                                                                tgttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAggcgg                                                                                                                 >  2:234244/1‑248 (MQ=255)
                                                                                                                                 gttgGTAACAATGCGCAAATGGCTGTGATTGAGCGGTTCGTTCGCTACCGC‑TCCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                 <  1:445713/250‑1 (MQ=255)
                                                                                                                                    ggTGACAATGCGAAAATTGGTGTGATGGAGCAGTGCGTGCGCTCCCCC‑TTCCGGCGTGGTG‑CGAATAGC‑GATAAACAGCGTCGAGCCATGGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                                                                             <  2:147911/251‑1 (MQ=255)
                                                                                                                                     gTGACAATGCGCAATGTGTTGTGATTGAGCATGGCTTGCGCTCCCGC‑TTCCGGTGGGTTC‑CCGATATC‑GATAAACAGCGTCTGGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACAATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                            <  1:129072/251‑1 (MQ=255)
                                                                                                                                         aaaTGCGCAATTTGCTGTGATTGGGCAGTGCTTGCGCTACCGT‑TCCCGGCGTGGTG‑CCTATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CCCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                        <  1:195134/250‑1 (MQ=255)
                                                                                                                                          aaTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                         >  1:3819/1‑249 (MQ=255)
                                                                                                                                                   atttgtgtgATGGATCAGGGCGTGCGCTCCCGC‑TTC‑GGGGTGGGGGCCGATAGC‑GATAAACAGCGTCGGGCCATGGGGGA‑TT‑TG‑CCCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                              <  2:173127/246‑1 (MQ=255)
                                                                                                                                                     tgctggaattgtggaGTGCGTGCGCTCCCGC‑TTCCGGTGTGGTG‑CCAATATC‑GATAAACATGTTGGAGCCATTGGTGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCTTTGCGATCGTGCCACTGCGT‑GTTAACCGACCTGGAAGGCAGCGCCGCTCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                                                                             <  1:362445/236‑1 (MQ=255)
                                                                                                                                                        tgtgATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGCTATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCTGCCACTTTGAGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                         >  1:205878/1‑251 (MQ=255)
                                                                                                                                                          ggatggatcagggcgtgccCTCCCGC‑TTCGGGGGTGGTG‑CCGATATC‑AATAAACGGCGTGGGGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTGTTCTTCGGTCTGGGTGGCCTTGCGACTGTGCCACGGGGG‑GTTAACCGAACTGGAAGGCGGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga                                                                                       <  1:463262/239‑1 (MQ=255)
                                                                                                                                                           gATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                        >  2:154072/1‑249 (MQ=255)
                                                                                                                                                                 gcagtgggtggccTACCGC‑TTCGGGGGTGGGG‑CCGATATC‑GAAAAACAGGGTCGAGCCTTGGGGGT‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                                <  1:128248/239‑1 (MQ=255)
                                                                                                                                                                             cttccGC‑TTCAGGCGTGGTG‑CCGATATC‑GATAAATAGCGTCGAGCAATTGGGGA‑TG‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCACCGCACCGCCACGATGGAG‑CAGGAGCAGGTTTGGCTCCGCCAGCACATTGAGGTCGCGGCGAATAGTCTGCGAGCTGACGGAGCAATGctcgacaaacttt                                                                    >  2:7853/4‑241 (MQ=255)
                                                                                                                                                                               cccGC‑TTCGGGCG‑GGTGGCCGATATC‑GATAAACGGCGTCGGGCCATTGGGTA‑TT‑GG‑CTCCGCCCCTTTGCGGACGATGCGCTCTTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                   <  2:396430/249‑1 (MQ=255)
                                                                                                                                                                                       cggggggggtg‑CAGATACC‑GATAAACAGCGGCGA‑CCAATGGGGG‑TT‑TGTCTCCCCCACTTTGCGGACGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGT‑GTTAACCGAACTGGAAGGCATCGCCGCTCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCCTCGGTACTg                                                           <  2:272174/244‑1 (MQ=255)
                                                                                                                                                                                        cccggcggggtgcagttacggATAAAAAGCGTCGGGCATTTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                             <  1:132688/230‑1 (MQ=255)
                                                                                                                                                                                        cGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCAATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTg                                                           >  2:322316/1‑250 (MQ=255)
                                                                                                                                                                                         ggCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTg                                                                                                                                       >  1:117622/1‑173 (MQ=255)
                                                                                                                                                                                         ggCGTGGTG‑CCGATATC‑GATAAACAGCGGCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACAAGCTCTTCGGTACTGAc                                                         >  2:406196/1‑251 (MQ=255)
                                                                                                                                                                                             tggtg‑CCGATTTC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑GG‑CCCCGCCCTTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                  <  1:137968/238‑1 (MQ=255)
                                                                                                                                                                                             tggtg‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTc                                                                  >  2:137968/1‑238 (MQ=255)
                                                                                                                                                                                             tggtg‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa                                                      >  1:291649/1‑250 (MQ=255)
                                                                                                                                                                                              ggggtgcgattatcaaaaaccggcttCGACCCATTGGGTA‑TT‑GG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa                                                      <  2:207400/226‑1 (MQ=255)
                                                                                                                                                                                                tg‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                  >  2:239142/1‑251 (MQ=255)
                                                                                                                                                                                                       tatC‑GATAAACAGCGTCGAGCCATTGGGGC‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCTCGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGACATAACCCTGCTGt                                            >  2:516867/1‑251 (MQ=255)
                                                                                                                                                                                                               aaccgccggcgcgcttttGGGGA‑TT‑TG‑CCCCGCCACTTTGCGGCCGATGCCCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTGACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa                                     <  1:406196/235‑1 (MQ=255)
                                                                                                                                                                                                               aaacagggtcttgCCATTGGGTA‑TT‑TG‑CTCCTCCCCTTGGCGTGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGACCGGCCCCCGGCTT‑TTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa                                     <  2:43999/239‑1 (MQ=255)
                                                                                                                                                                                                                 aCAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                                   >  2:153858/1‑251 (MQ=255)
                                                                                                                                                                                                                    gcgccGAGCCATTGGGGA‑TT‑TG‑CGCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                                <  1:322316/247‑1 (MQ=255)
                                                                                                                                                                                                                        gggggccttGGGGAGTT‑GG‑CTCCGCCGCTTGGCGGACGAGGGGCTCTTTTTTTTGGGTCGGGGTGGCCTTGCGACCGGGCCACGGCGT‑GTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata                             <  2:385997/244‑1 (MQ=255)
                                                                                                                                                                                                                        ccgctctgttgggA‑TTGTG‑CTCCGCCACTTGGCGGCCGCTGCGCTTTTTTTCGTCGTTCGGGGTGGCGTTGCGATCGTGCCACGGCGT‑GTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGATAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                                <  2:129572/238‑1 (MQ=255)
                                                                                                                                                                                                                          aGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                           >  1:250525/1‑250 (MQ=255)
                                                                                                                                                                                                                             ggcctgtgggtattggccccGCCCCTTGGCGGCCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGACCGTGCCCCGGCGT‑GTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                          <  2:447831/233‑1 (MQ=255)
                                                                                                                                                                                                                               cctttggggtttgcccccGCCCCTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGT‑GTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc                        <  2:3819/235‑1 (MQ=255)
                                                                                                                                                                                                                                tGGGGA‑TT‑TC‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                     <  1:490658/250‑1 (MQ=255)
                                                                                                                                                                                                                                  gggA‑TT‑TG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg                  >  1:231322/1‑251 (MQ=255)
                                                                                                                                                                                                                                   tggggattggcgcagccatgttGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGGGT‑GTTAACCGAACTGGAAGGCAGCGCGGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                     <  1:488123/230‑1 (MQ=255)
                                                                                                                                                                                                                                    ggggtttagtctcGCCACTTTGCGGCCGGTGCGCGCTTTTTCTTCGTTCGGGGTCCCCTTCCGATCGTGCC‑CGGGGTTCTAAACCGATCTGGACGGCAGCTCCTCACCGCCATGATGG‑CGCAGGATCTGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGTCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAGCCAGTTCGATAATACCGTtgt                   <  2:451410/239‑1 (MQ=255)
                                                                                                                                                                                                                                             cTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGc                                                                       >  2:367328/1‑190 (MQ=255)
                                                                                                                                                                                                                                             cTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGc                                                                       <  1:367328/190‑1 (MQ=255)
                                                                                                                                                                                                                                               ccGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt        >  1:111648/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     cTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCTTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt    <  1:150554/249‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttGCGGACGATGCGCTCTTTTTCTTCGTTCTGGGTGGCCTTGCGATCGGGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCATGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc  <  2:250525/249‑1 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    
CGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTT‑AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGGTG‑CCGATATC‑GATAAACAGCGTCGAGCCATTGGGGA‑TT‑TG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGT‑GTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTC  >  NC_000913/3560116‑3560603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑