Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,507,249	C→T	D30N (GAC→AAC)	yhfT ←	uncharacterized protein YhfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,249	0	C	T	100.0%	155.7 / NA	43	D30N (GAC→AAC)	yhfT	uncharacterized protein YhfT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/22);  total (21/22)

GGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTG  >  NC_000913/3507012‑3507492                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    ggATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAGGCGATACTCCCCGCCTCGCGCCGGTTCATATAGCCTTCAATCAGTTGCGGCAGGTCCGGGCGGATGCCGTTATGAAAAACAgcc                                                                                                                                                                                                                                        >  2:266012/1‑251 (MQ=255)   aTCAACACGCCCCAGATAGCGCCAAGACCAAACGCAATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAgccgc                                                                                                                                                                                                                                      <  1:260360/251‑1 (MQ=255)     cAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCACAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCACTCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACACccgccg                                                                                                                                                                                                                                    >  2:180864/1‑251 (MQ=255)             cccAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATgc                                                                                                                                                                                                                            <  1:87344/251‑1 (MQ=255)             cccAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATgc                                                                                                                                                                                                                            <  1:71929/251‑1 (MQ=255)                       gcCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCCCCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGAGCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCg                                                                                                                                                                                                                  <  1:211040/251‑1 (MQ=255)                                               gTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAgccg                                                                                                                                                                                                                                       <  1:525483/206‑1 (MQ=255)                                                  aaTCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTGTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGGTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGccacc                                                                                                                                                                                        <  1:301199/249‑1 (MQ=255)                                                   aTCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCATTGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATTCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCcaccac                                                                                                                                                                                      <  1:104620/251‑1 (MQ=255)                                                   aTCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGccacca                                                                                                                                                                                       <  2:206089/250‑1 (MQ=255)                                                   aTCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCcaccac                                                                                                                                                                                      >  1:99503/1‑251 (MQ=255)                                                      cccAGGACGCCGAGGATATCGGGAGGAAGAAAGAGTAACCATGCGTTGAGCAGGCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGAt                                                                                                                                                                                   <  1:266012/250‑1 (MQ=255)                                                                     atatCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAATCCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCCCCACGATAATCTGAATATACAg                                                                                                                                                                    <  1:216408/251‑1 (MQ=255)                                                                            tAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCACATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCCCGCCACCACGATAATCTGAATATACAGATCCAt                                                                                                                                                              >  2:87426/1‑250 (MQ=255)                                                                                     agagTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAAGGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCAGATATCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCTTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCt                                                                                                                                                    <  1:550255/251‑1 (MQ=255)                                                                                       agTAACCATGCGTGGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGCCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGGCGTGCCttt                                                                                                                                                  >  1:350817/1‑251 (MQ=255)                                                                                          aaCCATGCTTTGAGAAGCCCGGTTTTCAGGTTAAAAGAGATCCCCCCCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGAACGCCACCACGATTATCTGAATATACAGAACCTTAGTCGTGCCTTTTAt                                                                                                                                               <  2:119269/251‑1 (MQ=255)                                                                                                     gAGCAGCCCGGTTTTCAGGGTAAAAGAGAACCCCACCGAGCCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCTGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTAGTTCATATGGt                                                                                                                                     <  1:470790/250‑1 (MQ=255)                                                                                                         agaccagtcataaggtgaaaaGAGATCCCCACCGATTCCACAAAACCATTGCACAGACCAAAGTCGATTCCCCCCGCCTCGCGAAGGTTCATAGAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATTAAAAACAGCCGCCGAGCGATGCTCCAGAAGCGAGGTCATACCCGTCAGGCACACCTCCACGATAATCTGAATATACTGTACCATAGTCGTGCCTGGTATTTCAGATGGTTAATc                                                                                                                                <  2:494781/234‑1 (MQ=255)                                                                                                          cccaggTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATTCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCa                                                                                                                               <  1:317326/247‑1 (MQ=255)                                                                                                                   tCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGa                                                                                                                       >  2:442888/1‑250 (MQ=255)                                                                                                                     aGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATc                                                                                                                    >  1:282061/1‑251 (MQ=255)                                                                                                                              gagaTCCCCCCCGAGGCCACAAACCAAAGGCTCAGACCAAAAGCGATTCTCCCCGCCTCGCGCCGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCg                                                                                                           <  2:301598/251‑1 (MQ=255)                                                                                                                                  tCCCCACTGAGGCCACACAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGCTCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGCTGCGCCAGCAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATTCCGTTCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGt                                                                                                       >  2:415701/1‑251 (MQ=255)                                                                                                                                  tCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGt                                                                                                       >  1:259121/1‑251 (MQ=255)                                                                                                                                              ccACAAAACCCATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGAAGGTTCATATAGCCTTCACTCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCCGAAGCGCTGTCATACCCGTCAGGCAAGCCACCACGCTAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACggca                                                                                            >  1:548213/1‑248 (MQ=255)                                                                                                                                                   aaaCCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGCAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGcc                                                                                       >  2:443977/1‑250 (MQ=255)                                                                                                                                                         aTGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGcc                                                                                >  1:398029/1‑251 (MQ=255)                                                                                                                                                                 aCCAAAAGCGATACTCCCCGCATCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTcc                                                                        >  2:316740/1‑251 (MQ=255)                                                                                                                                                                    aaaaGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTc                                                                     <  2:154304/251‑1 (MQ=255)                                                                                                                                                                                        cgcgACGGTTCATATAGCCTTCAAGCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCAGAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGat                                                   <  1:441797/249‑1 (MQ=255)                                                                                                                                                                                         gcgACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATg                                                >  1:274333/1‑251 (MQ=255)                                                                                                                                                                                         gcgACGGTTCATATAGCCTTCAAGCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATg                                                <  1:316740/251‑1 (MQ=255)                                                                                                                                                                                           gACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGCTGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCGGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGATCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGGTATGCt                                              >  1:26649/1‑251 (MQ=255)                                                                                                                                                                                           gACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAAAGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCt                                              >  2:427535/1‑251 (MQ=255)                                                                                                                                                                                           gACGGTTCATATAGCCTTCAATCAGTGGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCt                                              <  2:19131/251‑1 (MQ=255)                                                                                                                                                                                                               aTCAGTTGCGGCAGGATCTGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCTGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTGCCTGCAGCGATCATTTTGGCGATATGCTCGCCTTTCGCTTTAATGCCt                          <  2:548213/251‑1 (MQ=255)                                                                                                                                                                                                                     tGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTCATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTg                    >  2:339355/1‑251 (MQ=255)                                                                                                                                                                                                                     tGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTg                    >  2:291805/1‑251 (MQ=255)                                                                                                                                                                                                                        ggCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGc                  >  1:317455/1‑250 (MQ=255)                                                                                                                                                                                                                           aGGATAGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATAACCGTCAGGCACGCCTCCACGATAATCTGAATATACAGATACATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCACAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTACTGCCTGGTTTGGCAATg              >  2:237291/1‑251 (MQ=255)                                                                                                                                                                                                                            ggATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCCTCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATgg             >  1:549233/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTg  <  1:442888/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    GGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTG  >  NC_000913/3507012‑3507492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑