Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,5020CT98.3% 214.4 / NA 60T128I (ACT→ATT) crpDNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (35/24);  minor base G (0/1);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGG  >  NC_000913/3486263‑3486742
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
ggcagtgttgtccAAAGACGAAGAGGGTAAAGAAATGATCCCCTCCTTCCTGAATCAGGGTGATTTTATTGGCAAACGGGCCCT‑TTTGGAAG‑AGGGCAAGGAA‑CGTAGCGCATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCGGCAAGTCATTTCAGAGAAAg                                                                                                                                                                                                                                       <  1:180086/239‑1 (MQ=255)
  aaGTGCTGATCAAAAAGGAAGGGGGAAAAGAAATGATCCCCTCCAATCTGAATCAGGGTTGTTTTATGGGCGAACTGGGCCT‑GTT‑GAAGGAGGGCCAGGAA‑CGTGGCGGATGGGTACGGGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTg                                                                                                                                                                                                                                     <  1:206393/250‑1 (MQ=255)
           acAAAGACGAAGAGGGTAAAAAAATGACCCTCTCCTACCGTAATCAGGGTGTTTTTATGGGCGACGTGGGCCT‑TTTTGAAG‑AGGGCC‑GGAAGCGTAGCGCAGGGGTGCGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGGCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTg                                                                                                                                                                                                                            <  2:204785/250‑1 (MQ=255)
                 aCGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGCATCAGGGTGATTTTATTGGCGCACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTCCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTc                                                                                                                                                                                                                      >  2:42956/1‑251 (MQ=255)
                   gAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCt                                                                                                                                                                                                                    >  1:319686/1‑251 (MQ=255)
                    aaGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTc                                                                                                                                                                                                                   >  1:81753/1‑251 (MQ=255)
                     agagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGTTTTTATTGGCGCACTGCGCCT‑GTTTGAAG‑CGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCg                                                                                                                                                                                                                  >  2:360748/1‑251 (MQ=255)
                        gggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACg                                                                                                                                                                                                               >  2:228740/1‑251 (MQ=255)
                                      tCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACATGGCGTTCCTCGACGTGACGGGCCGCAtt                                                                                                                                                                                                 >  2:221457/1‑251 (MQ=255)
                                           tcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcac                                                                                                                                                                                             >  1:45318/1‑250 (MQ=255)
                                               aTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAct                                                                                                                                                                                        >  2:302428/1‑251 (MQ=255)
                                                tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGActc                                                                                                                                                                                       >  1:350444/1‑251 (MQ=255)
                                                  tGAATCCGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAgtatctg                                                                                                                                                                                     >  2:157593/1‑245 (MQ=255)
                                                     atcggggggATTTTATTGGCGAAC‑GGGCCTTGTTTGAAG‑GGGGCCAGGAA‑CGTGGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctgctg                                                                                                                                                                                  <  1:228740/243‑1 (MQ=255)
                                                         gggTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATc                                                                                                                                                                              >  1:204481/1‑251 (MQ=255)
                                                                tttATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGCATCTGGCaa                                                                                                                                                                        >  1:291099/1‑250 (MQ=255)
                                                                   aTTGGCGAACAGGGCCT‑GTTTGAAG‑AGGGACAGGAA‑CGTAGAGCATGGGAACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAACATTTCGCCCATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTAGTCTGCAAGTCATTTCAGAGACAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGCATCTGGCACaac                                                                                                                                                                     >  2:230069/1‑250 (MQ=255)
                                                                         gAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg                                                                                                                                                                                                >  1:521778/1‑217 (MQ=255)
                                                                               ggctt‑GTTTGAAG‑GGGGCAGGGAA‑CGTAGCGAATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCAGTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCGGCTGAATCTGGCAAAACAACCAGACGCTAt                                                                                                                                                        <  2:100622/247‑1 (MQ=255)
                                                                               ggCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTAt                                                                                                                                                        >  1:275742/1‑251 (MQ=255)
                                                                                 ctt‑GTTTGAAG‑AGGGCCAGGAA‑CGTGGCGAATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg                                                                                                                                                                                                <  1:359946/207‑1 (MQ=255)
                                                                                 ccT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg                                                                                                                                                                                                >  2:359946/1‑209 (MQ=255)
                                                                                              agGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGCAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGAc                                                                                                                                           >  1:275822/1‑251 (MQ=255)
                                                                                              agGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGAc                                                                                                                                           >  1:68746/1‑251 (MQ=255)
                                                                                                 gCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGCCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCACAACAACAAGACGCTATGACTCACCCGGACGGt                                                                                                                                        >  1:370305/1‑251 (MQ=255)
                                                                                                   cAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGCCGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGatat                                                                                                                                      >  2:347229/1‑251 (MQ=255)
                                                                                                      gaactggaggggaggtgaacgcgcaaaaaCCGCCTGTGAAGAGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGGTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAATCAGACGCTATGACTCGCCCCGACGGTATGc                                                                                                                                    <  2:77227/226‑1 (MQ=255)
                                                                                                             gtggcaggggaacgttgaaaaaCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAGTCAGGTAAACCCGGACATTCTGATGCGTTTGTCGGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCGGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCa                                                                                                                              <  2:297082/232‑1 (MQ=255)
                                                                                                                   tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTAc                                                                                                                       >  1:176563/1‑251 (MQ=255)
                                                                                                                    gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTAcc                                                                                                                      >  2:31538/1‑251 (MQ=255)
                                                                                                                      gaacggggaaaaaCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCg                                                                                                                    <  2:322692/242‑1 (MQ=255)
                                                                                                                              gAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTg                                                                                                                                                                                                                                     >  2:293730/1‑130 (MQ=255)
                                                                                                                                 aaCCGCCTGTGAAGGGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAGGGCGCGTCGTCTGCAAGTCATTTCAGATAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAAttc                                                                                                         <  2:297547/251‑2 (MQ=255)
                                                                                                                                           gAAGTGGCTGACATTTCGTACAAAAAATTTCGCCAATTGATTCAGGCAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACCACCAGACGCTCTGACTCACCCGGACGGTATGCAAATCAAACTTACCCGTCAGGAAATTGGTCAGATTGt                                                                                               >  2:70729/1‑251 (MQ=255)
                                                                                                                                           gAAGTGGCTGAAAGTTCGTACAAAAAATTACGCCAATGGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTg                                                                                                <  1:472473/250‑1 (MQ=255)
                                                                                                                                                ggCTGAAATTGCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGGCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCGCCCGGACGGTATGCAAATCAAAATTACCCGGCAGGAAATTGGACAGATTGTCGGCt                                                                                          <  2:542901/251‑1 (MQ=255)
                                                                                                                                                     aaaTTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtct                                                                                     <  1:221457/251‑1 (MQ=255)
                                                                                                                                                              aCAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAAcc                                                                            <  1:302428/251‑1 (MQ=255)
                                                                                                                                                                         cGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTGTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCa                                                                  <  2:3527/250‑1 (MQ=255)
                                                                                                                                                                                 gATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGa                                                         <  2:291099/251‑1 (MQ=255)
                                                                                                                                                                                     cAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCt                                                     >  1:221623/1‑251 (MQ=255)
                                                                                                                                                                                     cAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGAGTTCCTCGAAGTGACGGGCCGCATTGCACAGACTCTGATGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCACATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCt                                                     >  1:75629/1‑251 (MQ=255)
                                                                                                                                                                                       ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAGTTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTgg                                                   >  1:205137/1‑251 (MQ=255)
                                                                                                                                                                                         tAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa                                                  >  1:141112/1‑250 (MQ=255)
                                                                                                                                                                                            aCCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa                                                  >  1:435142/1‑247 (MQ=255)
                                                                                                                                                                                              ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCa                                            <  1:3585/251‑1 (MQ=255)
                                                                                                                                                                                              ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGGGGGACGCATTCTGAATATGCTGGAAGATCa                                            <  1:447387/251‑1 (MQ=255)
                                                                                                                                                                                                ggACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACATGGCGTTCATCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTAACCGTCAGGAAATTGGACAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGCTGCTGGAAGATc                                             >  1:74972/1‑248 (MQ=255)
                                                                                                                                                                                                         gATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtct                                 <  1:135218/251‑1 (MQ=255)
                                                                                                                                                                                                             cGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                            >  1:473317/1‑188 (MQ=255)
                                                                                                                                                                                                             cGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                            <  2:473317/188‑1 (MQ=255)
                                                                                                                                                                                                               tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTTGAAGATCAGAACCTGATCTCCGcac                           >  2:168873/1‑251 (MQ=255)
                                                                                                                                                                                                               tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcac                           >  1:51137/1‑251 (MQ=255)
                                                                                                                                                                                                                  gTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCacac                         <  1:30791/250‑1 (MQ=255)
                                                                                                                                                                                                                      gCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACgg                       <  2:221623/248‑1 (MQ=255)
                                                                                                                                                                                                                            aTGGCGCGTCGTCGGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCACCGCACACGGTAAAACCAt              <  2:81753/251‑1 (MQ=255)
                                                                                                                                                                                                                                 gcgTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGGTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCTGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGAGTGTCGGCTGTTCTCGTGAAACCGTGTGACGCATTCTGAAGATGCTGGAAGATCAGAACCGGATCTCCGCACACGGTAAAACCAccgtcg         <  2:75605/251‑1 (MQ=255)
                                                                                                                                                                                                                                     cgtcTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGttt      <  2:10217/250‑1 (MQ=255)
                                                                                                                                                                                                                                        cggcaggTCATTTCAGAGAACGTGGGCAACCTGGCGTTCCTCGACTTTACGTTCCGCATTGCACAGACTCTGCTGAATCTGGCCAAACAACCAGACGCTATTACTCACCCGCACGGTCTGCAATTCAACATTACCCGTCAGGAATTTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTTGTACGCATTCTTAAGATTCTTTAAGTTCAGAACTTTATCTCCGCTCACGGTaattccttcgttgtttttgg  >  2:324495/7‑233 (MQ=255)
                                                                                                                                                                                                                                        cTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGCAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACgg  >  2:324481/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
GGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT‑GTTTGAAG‑AGGGCCAGGAA‑CGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGG  >  NC_000913/3486263‑3486742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑