Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,175	G→A	C19Y (TGC→TAC)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,175	0	G	A	100.0%	206.3 / NA	56	C19Y (TGC→TAC)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

AAAGCTATGCTAAAACAGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTG  >  NC_000913/3485931‑3486404                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       aaaGCTATGCTAAAACAGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATCACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGATCTTGTCTCATTACCACAt                                                                                                                                                                                                                                  >  1:77227/1‑251 (MQ=255)     cTATGCTAAAACAGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCAt                                                                                                                                                                                                                              >  1:227540/1‑251 (MQ=255)       aTGCTAAAACAGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTCCCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCGTATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATa                                                                                                                                                                                                                             <  2:293512/250‑1 (MQ=255)       aTGCAAAAACAGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAACCAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATa                                                                                                                                                                                                                             <  1:16228/250‑1 (MQ=255)        tGCTAAAACAGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATa                                                                                                                                                                                                                             >  1:38675/1‑249 (MQ=255)            aaaaCAGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTAcc                                                                                                                                                                                                                       <  1:352284/251‑1 (MQ=255)             aaaCAGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTAccc                                                                                                                                                                                                                      >  2:271298/1‑251 (MQ=255)                 aGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGAAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATcc                                                                                                                                                                                                                  >  1:75605/1‑251 (MQ=255)                          gCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACg                                                                                                                                                                                                         >  2:189167/1‑251 (MQ=255)                             aCAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGc                                                                                                                                                                                                        <  1:181459/249‑1 (MQ=255)                                gTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTAtt                                                                                                                                                                                                   <  2:134401/251‑1 (MQ=255)                                   attcATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCAc                                                                                                                                                                                                <  2:168187/248‑1 (MQ=255)                                            tGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGCAAGCTTATACCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGa                                                                                                                                                                                        >  1:542901/1‑250 (MQ=255)                                             gTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaa                                                                                                                                                                                       >  1:256306/1‑250 (MQ=255)                                              tACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                                                                                                                                                                      >  1:58649/1‑250 (MQ=255)                                                  gCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGc                                                                                                                                                                                  <  1:298878/250‑1 (MQ=255)                                                  gCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCg                                                                                                                                                                                 >  1:61166/1‑251 (MQ=255)                                                           cAAAGGACGTCACATTACCGTTCAGTACAGTTGATAGCCCCTTCCAAGGGAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATATGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                                                                                                                                                                        <  2:357325/251‑1 (MQ=255)                                                                             cgtgccgtaacgTTGATCGCCCCTTCCCAGGCAGCGGGACGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGc                                                                                                                                                      >  2:371133/12‑251 (MQ=255)                                                                                gCAGTCCAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGTCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACAGTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct                                                                                                                                                   <  1:72183/251‑1 (MQ=255)                                                                                    tACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTgg                                                                                                                                               >  1:3527/1‑251 (MQ=255)                                                                                        gTTGATAGCCCCTTCCAAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACCTCGTTAAAGGCTCTGTGGCAGt                                                                                                                                           >  2:180086/1‑251 (MQ=255)                                                                                              aGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGAt                                                                                                                                     >  1:297082/1‑251 (MQ=255)                                                                                                 cccTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCa                                                                                                                                   >  1:132803/1‑250 (MQ=255)                                                                                                    ttCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCACACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaa                                                                                                                                  >  2:206393/1‑248 (MQ=255)                                                                                                     tccaaGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGa                                                                                                                               <  2:226464/246‑1 (MQ=255)                                                                                                     tCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAg                                                                                                                                <  2:233718/249‑1 (MQ=255)                                                                                                      cccAGGTAGGGGGAAGAATATTTCGGCAATCCAGAGAAAGCGGGGGTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACg                                                                                                                             <  2:374881/251‑1 (MQ=255)                                                                                                           gTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATGCACCAGGGTGAAAAAGCGGAAACGCTGTACGACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAagag                                                                                                                        <  1:65858/251‑1 (MQ=255)                                                                                                              gCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGt                                                                                                                     <  2:61166/251‑1 (MQ=255)                                                                                                                       atatTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATg                                                                                                           >  1:408936/1‑251 (MQ=255)                                                                                                                             cGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACcaca                                                                                                                                                                                                                                   >  1:63160/1‑126 (MQ=255)                                                                                                                             cGGCAATCCAGAGACAGCGGCGTTATCTGGCTCGGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACcaca                                                                                                                                                                                                                                   <  2:63160/126‑1 (MQ=255)                                                                                                                              ggCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCctct                                                                                                    <  1:189167/251‑1 (MQ=255)                                                                                                                                 aaTCCAGAGACAGCGGAGTTATCTGGATCTGGAGAAAGCTTATACCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAACGCGGCAACGCTGTACTAAATCGTTAAAGGCTCTGTGGCAGTGCTGATCAACGACGAAGAGGGTAAA‑GAAATGATCCTCTCCt                                                                                                 >  2:517746/1‑251 (MQ=255)                                                                                                                                  aTCCAGAAACAGCGGCGGTATCTGGCTATGGAGAAAGCTTATAACAGCGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGATAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GACATGATCCTCTCCt                                                                                                 >  2:30791/1‑250 (MQ=255)                                                                                                                                      agagACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTg                                                                                            <  2:96623/251‑1 (MQ=255)                                                                                                                                                  gTTATCTGGCTCTGGAGAAAGCTTATCACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTa                                                                                                                                                                                                     >  2:261998/1‑135 (MQ=255)                                                                                                                                                  gTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTa                                                                                                                                                                                                     <  1:261998/135‑1 (MQ=255)                                                                                                                                                        gggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCGTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt                                                                          <  2:227540/250‑1 (MQ=255)                                                                                                                                                         ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCGCATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCGGTACGACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt                                                                          <  1:371133/250‑1 (MQ=255)                                                                                                                                                         ggCTATGGAGAAAGTTTATAACAGAGGATAACCGCGCATGGTGCTGGGCAAACCGCAAACAGACCCGACACTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTa                                                                            <  1:525812/248‑1 (MQ=255)                                                                                                                                                               ggAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACGCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGa                                                                     <  1:271298/249‑1 (MQ=255)                                                                                                                                                                                  ggATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAggg                                                <  2:376373/251‑1 (MQ=255)                                                                                                                                                                                         cgcgCAGGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTAGCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGaa                                         <  2:159413/251‑1 (MQ=255)                                                                                                                                                                                                ggTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAgcg                                  >  1:321700/1‑251 (MQ=255)                                                                                                                                                                                                   gCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTAGCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAt                               <  2:408936/251‑1 (MQ=255)                                                                                                                                                                                                     ttGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATgg                             >  1:264269/1‑251 (MQ=255)                                                                                                                                                                                                        gCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGt                           >  1:100622/1‑250 (MQ=255)                                                                                                                                                                                                         cAAACCGCACCCAGGCCCGCCTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTTCCCATCCAAGAGCACGCTTATTCTCCCGTGTGAAAAAGCGGAAACGCTGTCCTTCATCTTTAAAGGCTCTGTGGCTTTGCTGATCAAAGACGAAGAGTGTAAA‑GAGATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAAGGGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTAc                         <  1:220225/251‑1 (MQ=255)                                                                                                                                                                                                              cGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCCGGGTGCAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCg                    >  1:372073/1‑251 (MQ=255)                                                                                                                                                                                                                  aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGaaa                 >  1:426378/1‑250 (MQ=255)                                                                                                                                                                                                                    cAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAAcc              >  1:527064/1‑251 (MQ=255)                                                                                                                                                                                                                     aGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCg             >  1:297547/1‑251 (MQ=255)                                                                                                                                                                                                                            aCTCTCGAATGCTTATTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGACAAACCGGAAACGCTGTAATCCATCGTTAAAGGCTCTGTGGCAGGGCTGATCAAAGAGGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGGAGAGGGCCAGGAACGTAGCGCAGGGGTAAGTGCGAAAACTGACtgtgg      >  2:72194/1‑250 (MQ=255)                                                                                                                                                                                                                                aagAATGGTTATGGTCTCATTACCACATTCAAAAGCACCCATCAAAGAGCACGTTTATTCACCAGGGTGAAAAAGCGGAAACGCTGAACTACACCGTAAAAGGCTCTGGGGCAGGGGTGATAAAAGAAGAAG‑GGGTAAAAGAAATGATCCTCTCCTATCTGAAGAAGGGTGTTTTTATTGGCAAACTGGGCCTGTTGGAAGAGGGCCAGGAACGTAGCGAATGGGGACGTGCAAAAACCGCCTGTGAAGTg  <  2:113737/249‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       AAAGCTATGCTAAAACAGTCAGGATGCTACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAA‑GAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTG  >  NC_000913/3485931‑3486404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑