Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,444,922	G→C	T103S (ACC→AGC)	rpsE ←	30S ribosomal subunit protein S5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,444,922	0	G	C	100.0%	137.5 / NA	45	T103S (ACC→AGC)	rpsE	30S ribosomal subunit protein S5
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (24/21);  total (24/21)

GTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGAT  >  NC_000913/3444700‑3445167                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      gtgaTTTTAATAGTCTTTGCCATGGTTTATTTCCACAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTACTCGGGTTGGTGGAACCATAGTCTTAAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCGACCTTCGGAAGCCGCCTACATGAATa                                                                                                                                                                                                                            >  2:17778/4‑250 (MQ=255) gTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATAc                                                                                                                                                                                                                           <  1:478574/251‑1 (MQ=255)  tAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGCTTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATAcg                                                                                                                                                                                                                          >  2:540537/1‑251 (MQ=255)          aTAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAAcc                                                                                                                                                                                                                  <  1:540537/251‑1 (MQ=255)           tAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCCTTTCTGGAGAATTCATATTTTCCCGCCCATAAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGACCCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCCGAACGGCGCGCATTGCACCACCGGCGACGATCCCGCTACCTTCGGAAGCCGGCTGCATGAATACGCAAGAAccc                                                                                                                                                                                                                 >  1:35981/1‑251 (MQ=255)              tCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGCATTCATATTTTCCAGGCCATCAATAGTTGCACGCACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGAGCGCATTGCACCACCGGCGATGATACCGCTACCTTAGGAAGCCGGCTGCATGAATACGCGAGAACCCgtg                                                                                                                                                                                                              >  1:476056/1‑251 (MQ=255)                   gCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGaa                                                                                                                                                                                                          <  1:130221/250‑1 (MQ=255)                   gCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGaa                                                                                                                                                                                                          <  1:421502/250‑1 (MQ=255)                   gCCATGGTTTATTTCACCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAac                                                                                                                                                                                                         >  2:320990/1‑251 (MQ=255)                          tttATTTCCCCAGAATTTCTTCAACGGATATACCAAGCTTGACAGCGACCATTTCTGGACAATTCATATTTTCCAAGCCATCACTAGTTGCACGCAACACGTTCATCGGATTGATAGAACCAAAGGCTTTAGCAAGAACGATATCAACCACAGCGACTTCCAGAACGCAGCGCATTGCACCACCGGCGATGATACCGCTACCTGCGGAAGCCAGCTACATGCAGACGCGAGAATCCGCGTGAAAACCttt                                                                                                                                                                                                   >  1:33083/1‑250 (MQ=255)                                tCCCCAGAAGTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAAATCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCTCCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTATCAgg                                                                                                                                                                                             <  1:82058/250‑1 (MQ=255)                                    cAGAATTTCTTAAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACACCAGCGACATCCAGAACGGCGCGCATTGCACCACCGGCGCTGATACCGCAACCTTCGGACGCCGGCTGCATGCATAAGCGAGAACCCGTGTGAACACCTTTAACAGGgtgtg                                                                                                                                                                                        >  2:506794/1‑250 (MQ=255)                                          ttcttcGACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACAGCTACCTTAGGAAGCCGGCTGCATGAATACGCGAGAACACGTGTGAACACCTTTAACAGGGTGTTGCag                                                                                                                                                                                    >  1:449666/1‑249 (MQ=255)                                                  cGGATTTACCACGCTTGGCAGCGTTCATTTCTGGACAATTCATATTTTCCAGGCCATCAATAGTTTCACCAACCACGTTGATCGCGTTGGTGGAACCATAGTCTTTAGCCAGATCGTTATTAGTCCCAGCGACGTCCAGAACGGAGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGGTGCATGAATACGCCAGAACCCGTGTGAACCCCTTTAACAGGGTGTTGCAGAGTGCCGTTa                                                                                                                                                                          <  1:11870/251‑1 (MQ=255)                                                   ggATTTACCACGCGTGGCAGCGACCATTTCTTGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCGACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTAt                                                                                                                                                                         <  2:487772/251‑1 (MQ=255)                                                     aTTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTc                                                                                                                                                                       >  2:511596/1‑251 (MQ=255)                                                                 tGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGtt                                                                                                                                                            <  1:390162/250‑1 (MQ=255)                                                                 tGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAAGAGTGGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGtt                                                                                                                                                            <  1:320990/250‑1 (MQ=255)                                                                     cgCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATc                                                                                                                                                        >  2:487555/2‑250 (MQ=255)                                                                        gACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCatat                                                                                                                                                    >  1:392256/1‑251 (MQ=255)                                                                                          cATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTTGTGGAACCATAGGCTTTAGCCAGATCGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTTCACCTCCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCTTGTTAACATCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATAATATGGCGTCGGGCTTGTTCCa                                                                                                                                  <  2:500927/251‑1 (MQ=255)                                                                                            tatTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATc                                                                                                                                >  1:242930/1‑251 (MQ=255)                                                                                             atTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGA‑CCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACAACTTTAACAGGGGGTTGCAGAGGGCCGTTATTCAGCGCGGCGTTAATCATATTGCGACGGGCTTTTTCCATCGc                                                                                                                              >  1:92578/1‑251 (MQ=255)                                                                                                         aTCAATAGTTGAACGAACCACGTTGATCGGGTTGGAGGCACCATAGGCTTTAGCCAGACCGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGCACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTCATCTTATTGCGACGGGCTTTTTCCATCGCTTTCTGTATCg                                                                                                                   >  2:214884/1‑251 (MQ=255)                                                                                                           aaaTAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCAGAGTCTTGAGCCAGAACGTTATTAACCCCACCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATTATACCGCTACCTGCGGATGCCGGTTGCATGAATACGCGAGAACCCGTGTGAACACATTGAACATGGTGTTGCAGAGTGCCGGCATTCAGCGCGCCGTTAATCGTATTGCGACGGGCTGTTTCCGTCGCTTTCTGGAACgct                                                                                                                 <  2:226454/250‑1 (MQ=255)                                                                                                                gTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTgg                                                                                                            >  1:431195/1‑251 (MQ=255)                                                                                                                gTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTgg                                                                                                            <  2:261566/251‑1 (MQ=255)                                                                                                                                   tCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCttt                                                                                             >  2:89377/1‑247 (MQ=255)                                                                                                                                   tCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCttt                                                                                             <  1:89377/247‑1 (MQ=255)                                                                                                                                      ggTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaa                                                                                      <  1:114913/251‑1 (MQ=255)                                                                                                                                                 ccATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACATGGTGTTGCAGAGTGCCGTTATTCAGCGCGTCGTTAAGCATATTGCTACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAAc                                                                           <  1:83370/251‑1 (MQ=255)                                                                                                                                                   aTAGGCTTTAGCCAGAACGTTAGGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAAcgc                                                                         <  2:392256/251‑1 (MQ=255)                                                                                                                                                    tAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAAcgcg                                                                        >  1:47851/1‑251 (MQ=255)                                                                                                                                                                        aTGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACt                                                     <  1:178840/250‑1 (MQ=255)                                                                                                                                                                               ccAGCGACTTCCAGACCGGCACGCATTGCACCCCGGGCGATGATACCGCTACCTTCGGAAGTCGGCTGCATGAATACGCGAGACGCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTGTTCCATCGCTTGCTGGATCGCTGCTGGAACTGCACGCGCTTTACCGTAACCAAAACCATCGCGACCGTTACCAGCGCCAACTACAGTCag                                             <  2:476056/251‑1 (MQ=255)                                                                                                                                                                                    gACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtg                                        <  1:511596/251‑1 (MQ=255)                                                                                                                                                                                          cAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAgg                                  >  1:120359/1‑251 (MQ=255)                                                                                                                                                                                                ggCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACCGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAACCCACCGCGCCCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGaaa                             >  2:19444/1‑250 (MQ=255)                                                                                                                                                                                                ggCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGCTCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGaaa                             >  1:479503/1‑250 (MQ=255)                                                                                                                                                                                                           caccacCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCt                 >  1:312699/1‑251 (MQ=255)                                                                                                                                                                                                                ccGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAAc            <  2:120359/251‑1 (MQ=255)                                                                                                                                                                                                                      atgatACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTCACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGtttt      >  2:65872/1‑251 (MQ=255)                                                                                                                                                                                                                        gatACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGGAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAg    <  2:185949/251‑1 (MQ=255)                                                                                                                                                                                                                          tACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCCTATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTCACCAAATCCAACGCGACCGTTACCATCGCCAACTACGGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGgttt      >  2:247861/1‑247 (MQ=255)                                                                                                                                                                                                                           aCCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTATGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGAt  >  1:487598/1‑250 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      GTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGAT  >  NC_000913/3444700‑3445167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑